Sox17 (NM_001289464) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR229814
Sox17 (myc-DDK-tagged) - Mouse SRY (sex determining region Y)-box 17 (Sox17), transcript variant 2
  $503.00
2 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Sox17
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR229814 representing NM_001289464
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCAGCCCGGATGCGGGATACGCCAGTGACGACCAGAGCCAGCCCCGGAGCGCGCAGCCCGCGGTGA
TGGCAGGGTTGGGCCCCTGTCCCTGGGCCGAGTCCCTGAGCCCCCTCGGGGATGTAAAGGTGAAAGGCGA
GGTGGTGGCGAGTAGCGGGGCGCCAGCCGGGACGTCGGGCCGAGCCAAAGCGGAGTCTCGCATCCGGCGG
CCGATGAACGCCTTTATGGTGTGGGCCAAAGACGAACGCAAGCGGTTGGCACAGCAGAACCCAGATCTGC
ACAACGCAGAGCTAAGCAAGATGCTAGGCAAGTCTTGGAAGGCGTTGACCTTGGCAGAGAAGCGGCCCTT
CGTGGAAGAGGCCGAGCGGCTGCGCGTGCAGCATATGCAGGACCACCCCAACTACAAGTACCGGCCGCGG
CGGCGCAAGCAGGTGAAGCGCATGAAGCGGGTGGAGGGAGGCTTCCTGCACGCTCTCGTCGAGCCCCAGG
CCGGCGCGCTTGGTCCCGAGGGCGGCCGCGTGGCCATGGATGGCCTGGGTCTGCCTTTCCCGGAGCCGGG
CTATCCGGCCGGTCCTCCGCTGATGTCTCCGCACATGGGCCCCCACTATCGGGACTGCCAGGGACTGGGC
GCTCCCGCGCTCGACGGCTACCCTCTGCCCACTCCGGACACATCCCCGCTGGATGGCGTGGAGCAGGACC
CGGCTTTCTTTGCAGCCCCGCTGCCAGGGGACTGCCCGGCGGCCGGCACCTACACTTACGCTCCAGTCTC
GGACTATGCAGTGTCCGTAGAGCCGCCCGCTGGCCCCATGCGAGTGGGGCCGGACCCCTCGGGCCCTGCG
ATGCCGGGGATCCTGGCGCCCCCCAGCGCTCTGCACCTGTACTACGGCGCGATGGGCTCGCCCGCCGCAA
GTGCGGGGCGCGGTTTCCACGCGCAACCCCAGCAGCCGCTGCAACCGCAGGCACCGCCGCCGCCACCGCA
GCAGCAGCACCCAGCGCACGGCCCCGGGCAACCTTCGCCCCCTCCCGAGGCTCTGCCCTGCCGGGATGGC
ACGGAATCCAACCAGCCCACTGAGCTCCTAGGGGAGGTGGACCGCACGGAATTCGAACAGTATCTGCCCT
TTGTGTATAAGCCCGAGATGGGTCTTCCCTACCAGGGACACGACTGCGGAGTGAACCTCTCAGACAGCCA
CGGAGCCATTTCCTCCGTGGTGTCCGACGCTAGCTCAGCGGTCTACTATTGCAACTACCCCGACATT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR229814 representing NM_001289464
Red=Cloning site Green=Tags(s)

MSSPDAGYASDDQSQPRSAQPAVMAGLGPCPWAESLSPLGDVKVKGEVVASSGAPAGTSGRAKAESRIRR
PMNAFMVWAKDERKRLAQQNPDLHNAELSKMLGKSWKALTLAEKRPFVEEAERLRVQHMQDHPNYKYRPR
RRKQVKRMKRVEGGFLHALVEPQAGALGPEGGRVAMDGLGLPFPEPGYPAGPPLMSPHMGPHYRDCQGLG
APALDGYPLPTPDTSPLDGVEQDPAFFAAPLPGDCPAAGTYTYAPVSDYAVSVEPPAGPMRVGPDPSGPA
MPGILAPPSALHLYYGAMGSPAASAGRGFHAQPQQPLQPQAPPPPPQQQHPAHGPGQPSPPPEALPCRDG
TESNQPTELLGEVDRTEFEQYLPFVYKPEMGLPYQGHDCGVNLSDSHGAISSVVSDASSAVYYCNYPDI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001289464
ORF Size 1257 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001289464.1, NP_001276393.1
RefSeq Size 3288 bp
RefSeq ORF 1260 bp
Locus ID 20671
UniProt ID Q61473
Cytogenetics 1 1.65 cM
MW 45.1 kDa
Summary This gene encodes a member of the Sox (Sry-related high mobility group box) family of transcription factors involved in the regulation of embryonic development. The encoded protein plays a role in the determination of cell fate and in maintaining cell identity. This gene regulates tumor angiogenesis and tumor progression. Mutations in the human gene are associated with vesicoureteral reflux, characterized by the backward flow of urine from the bladder into the ureters or the kidney. Alternative splicing results in multiple transcript variants. provided by RefSeq, Jan 2014
Reviews
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_001289464" in other vectors (1)
SKU Description Size Price
MC227603 Sox17 (untagged) - Mouse SRY (sex determining region Y)-box 17 (Sox17), transcript variant 2 10 ug
$457.00

Plasmid Map for MR229814

Download sequence for

Cloning scheme for MR229814

Circular map for MR229814

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products