Sox17 (NM_001289464) Mouse Untagged Clone
SKU
MC227603
Sox17 (untagged) - Mouse SRY (sex determining region Y)-box 17 (Sox17), transcript variant 2
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
|---|---|
| Target Symbol | Sox17 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites | SgfI-MluI | |
| ACCN | NM_001289464 |
| Insert Size | 1260 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001289464.1, NP_001276393.1 |
| RefSeq Size | 3288 bp |
| RefSeq ORF | 1260 bp |
| Locus ID | 20671 |
| UniProt ID | Q61473 |
| Cytogenetics | 1 1.65 cM |
| Summary | This gene encodes a member of the Sox (Sry-related high mobility group box) family of transcription factors involved in the regulation of embryonic development. The encoded protein plays a role in the determination of cell fate and in maintaining cell identity. This gene regulates tumor angiogenesis and tumor progression. Mutations in the human gene are associated with vesicoureteral reflux, characterized by the backward flow of urine from the bladder into the ureters or the kidney. Alternative splicing results in multiple transcript variants. provided by RefSeq, Jan 2014 Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein (isoform a, also known as Sox17; PMID 8636240). |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001289464" in other vectors (1)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MR229814 | Sox17 (myc-DDK-tagged) - Mouse SRY (sex determining region Y)-box 17 (Sox17), transcript variant 2 | 10 ug |
$503.00
|
|
Citations
Femtosecond laser assisted photo-transfection and differentiation of mouse embryonic stem cells
Thobakgale, Manoto, Ombinda Lemboumba et al
Optical Interactions with Tissue and Cells XXIX (2018)
Thobakgale, Manoto, Ombinda Lemboumba et al
Optical Interactions with Tissue and Cells XXIX (2018)
Snippet: A region of interest was marked using circular stickers of 20 mm diameter. Following this, approximately 50 cells within the roi were porated with the laser pulses in the presence of 50 µl of 15 µg/ml Sox 17 (Origene, MC227603) solution
Efficient femtosecond driven SOX 17 delivery into mouse embryonic stem cells: Differentiation study
Thobakgale, Manoto, Lemboumba et al
SPIE Proceedings (2017) 10068
Thobakgale, Manoto, Lemboumba et al
SPIE Proceedings (2017) 10068
Snippet: coated dishes. Before poration, a region of interest (roi) was marked on the plates and 50 to 100 cells within the roi were irradiated in the presence of 2ug/ml Sox 17 (Origene, MC227603) made with FBS-free KDMEM. The transfection ... All 2 versions Cite SaveSaving
Showing 1-2 of 2 papers.
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