Ptgs2 (NM_011198) Mouse Tagged ORF Clone
$843.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Ptgs2 |
| Synonyms | COX; Cox-; Cox-2; COX2; gripghs; PES-2; Pgh; PGHS; PGHS-2; Pghs2; PHS; PHS-2; PHS II; Tis; TIS10 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_011198 |
| ORF Size | 1812 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_011198.4 |
| RefSeq Size | 4150 bp |
| RefSeq ORF | 1815 bp |
| Locus ID | 19225 |
| UniProt ID | Q05769 |
| Cytogenetics | 1 63.84 cM |
| MW | 69.5 kDa |
| Summary | This gene encodes an enzyme that is a member of the prostaglandin G/H synthase family. The encoded protein converts arachidonic acid to prostaglandin endoperoxide H2 which is a key enzymatic step in prostaglandin biosynthesis. This gene is the inducible prostaglandin G/H synthase family member that is upregulated during inflammation. Aberrant regulation of this gene is associated with cancer progression in several tissues and an increased risk of cardiovascular events. This gene is the target of many non-steroidal anti-inflammatory drugs. provided by RefSeq, Sep 2015 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_011198" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC219616 | Ptgs2 (untagged) - Mouse prostaglandin-endoperoxide synthase 2 (Ptgs2), (10ug) | 10 ug |
$844.00
|
|
| MG227684 | Ptgs2 (tGFP-tagged) - Mouse prostaglandin-endoperoxide synthase 2 (Ptgs2), (10ug) | 10 ug |
$1,043.00
|
|
| MR227684L3 | Lenti ORF clone of Ptgs2 (Myc-DDK-tagged) - Mouse prostaglandin-endoperoxide synthase 2 (Ptgs2) | 10 ug |
$1,143.00
|
|
| MR227684L4 | Lenti ORF clone of Ptgs2 (mGFP-tagged) - Mouse prostaglandin-endoperoxide synthase 2 (Ptgs2) | 10 ug |
$1,143.00
|
|
Citations
Nicotinamide Mononucleotide Alleviates LPS-Induced Inflammation and Oxidative Stress via Decreasing COX-2 Expression in Macrophages
Liu, Zong, Zhang et al
Front Mol Biosci (2021) 8, 702107 DOI: 10.3389/fmolb.2021.702107
Liu, Zong, Zhang et al
Front Mol Biosci (2021) 8, 702107 DOI: 10.3389/fmolb.2021.702107
Cited by 51
AI Summary: OriGene’s Ptgs2 (NM_011198) Mouse Tagged ORF Clone (Clone ID MR227684) was used to generate COX-2-overexpression cell lines, enabling the authors to investigate the role of COX-2 in NMN-mediated suppression of inflammation and oxidative stress in macrophages.
Snippet: COX-2-Overexpression and COX-2-Knockdown Cell Lines To construct stable COX-2-Overexpression cell lines, mouse Cox2 cDNA was obtained from Ptgs2 (NM_011198) Mouse Tagged ORF Clone (Clone ID MR227684, OriGene). The cDNA was cloned into the plasmid pLVX-IRES-ZsGreen1 to create the pLVX-COX-2-IRES-ZsGreen1 vector. Empty pLVX-IRES-ZsGreen1 was used as negative control. Recombinant construct with three
Transferrin is responsible for mediating the effects of iron ions on the regulation of anterior pharynx-defective-1α/β and Presenilin 1 expression via PGE2 and PGD2 at the early stage of Alzheimer's Disease
Lu, Ma, Luo et al
Aging (Albany NY) (2018) 10 (11), 3117-3135 DOI: 10.18632/aging.101615
Lu, Ma, Luo et al
Aging (Albany NY) (2018) 10 (11), 3117-3135 DOI: 10.18632/aging.101615
AI Summary: OriGene products, specifically COX-2 and L-PGDS cDNA plasmids, were used in this study to investigate the molecular mechanisms by which iron ions and transferrin regulate key proteins involved in Alzheimer's disease.
Snippet: COX-2 or L-PGDS cDNA plasmids were obtained from Origene Technologies (Rockville, MD, USA), and subcloned to the pCMV6-XL vector. Antibodies against β-actin, PS1 and Aβ (Stock No. 15126) were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA)
In vitro functional characterization of prostaglandin-endoperoxide synthase 2 during chondrocyte hypertrophic differentiation
Li, Wang, Zhu et al
Oncotarget (2016) 7 (24), 36280-36292 DOI: 10.18632/oncotarget.8889
Li, Wang, Zhu et al
Oncotarget (2016) 7 (24), 36280-36292 DOI: 10.18632/oncotarget.8889
Cited by 3
Snippet: We have generated a Cox-2 expressing ATDC5 stable cell line using Cox-2 expression plasmid (Origene, MR227684) with pCMV6-entry control and G418 selection
Showing 1-3 of 3 papers.
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