Cbl (NM_007619) Mouse Tagged ORF Clone

SKU MR227679

Cbl (Myc-DDK-tagged) - Mouse Casitas B-lineage lymphoma (Cbl)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Cbl
• Synonyms: 4732447J05Rik; c-Cbl; Cbl-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227679 representing NM_007619; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCCGGCAACGTGAAGAAGAGCTCGGGCGCCGGCGGCGGCGGCTCTGGGGGCTCGGGAGCGGGCGGCC; TGATCGGGCTCATGAAGGACGCCTTCCAGCCGCACCACCACCACCACCACCTCAGCCCGCACCCTCCCTG; CACGGTGGACAAGAAGATGGTGGAGAAGTGCTGGAAGCTCATGGACAAGGTGGTGCGGTTGTGTCAAAAC; CCAAAGCTGGCGCTCAAGAACAGCCCGCCTTATATCTTAGACCTGCTGCCTGACACCTACCAGCACCTCC; GCACTGTCTTGTCAAGATATGAGGGGAAGATGGAGACGCTTGGAGAAAATGAGTATTTCAGGGTGTTCAT; GGAAAATTTGATGAAGAAAACTAAGCAGACTATCAGCCTCTTCAAGGAGGGAAAAGAAAGGATGTATGAG; GAGAATTCCCAGCCTAGGCGAAACCTGACCAAATTATCCCTGATCTTCAGCCACATGCTGGCAGAACTGA; AAGGCATCTTTCCGAGCGGACTCTTCCAAGGAGACACTTTCCGGATTACTAAAGCTGATGCTGCCGAATT; TTGGAGAAAAGCTTTTGGTGAAAAGACGATAGTCCCGTGGAAGAGCTTTCGACAGGCCCTGCATGAAGTG; CATCCCATCAGTTCTGGGCTGGAGGCCATGGCTCTGAAGTCCACTATTGATCTGACCTGCAATGATTATA; TTTCTGTCTTTGAATTTGATATTTTTACACGGCTGTTTCAGCCCTGGTCCTCTTTGCTCAGAAATTGGAA; CAGCCTTGCTGTAACTCACCCTGGTTACATGGCTTTCCTGACATACGATGAAGTGAAAGCGCGCCTGCAG; AAGTTCATCCACAAACCTGGCAGTTACATCTTTCGGCTGAGCTGTACTCGTTTGGGTCAGTGGGCTATTG; GGTATGTTACTGCCGATGGGAACATTCTGCAGACAATCCCACACAATAAACCGCTCTTCCAAGCACTGAT; TGATGGCTTCAGGGAAGGCTTCTATTTGTTTCCTGATGGACGAAATCAAAATCCTGACCTGACAGGTTTA; TGTGAACCAACTCCTCAAGATCATATCAAAGTAACCCAGGAACAATATGAATTATACTGTGAAATGGGCT; CCACATTTCAACTGTGTAAGATATGTGCTGAGAATGATAAGGATGTGAAGATTGAGCCCTGTGGACACCT; CATGTGCACATCCTGCCTCACGTCGTGGCAGGAATCAGAAGGTCAGGGCTGTCCTTTTTGCCGATGTGAA; ATCAAAGGTACTGAGCCCATCGTGGTGGATCCGTTTGACCCCAGAGGCAGTGGCAGCCTATTAAGGCAAG; GAGCAGAAGGTGCTCCTTCCCCAAATTACGACGATGATGATGATGAACGAGCTGATGATTCTCTCTTCAT; GATGAAGGAGTTGGCAGGTGCCAAGGTGGAAAGGCCTTCCTCTCCATTCTCCATGGCCCCACAAGCTTCC; CTTCCTCCAGTGCCACCAAGACTTGACCTTCTACAGCAGCGAGCACCTGTTCCTGCCAGCACTTCAGTTC; TGGGGACTGCTTCCAAGGCTGCTTCTGGCTCCCTTCATAAAGACAAACCATTGCCAATACCTCCCACACT; TCGAGATCTTCCACCACCACCCCCTCCAGACCGGCCTTACTCTGTTGGAGCAGAAACAAGGCCTCAGAGA; CGCCCTCTGCCTTGTACACCAGGCGATTGTCCATCTAGAGACAAACTGCCCCCTGTCCCTTCTAGCCGCC; CAGGGGACTCGTGGTTGTCTCGGCCAATCCCTAAAGTACCAGTAGCTACTCCAAACCCTGGTGATCCTTG; GAATGGGAGAGAATTGACCAATCGGCACTCGCTTCCATTCTCATTGCCCTCACAAATGGAACCCAGAGCA; GATGTCCCTAGGCTTGGAAGCACATTTAGTCTGGATACCTCTATGACTATGAATAGCAGCCCAGTAGCAG; GTCCAGAGAGTGAGCACCCAAAGATCAAGCCTTCCTCGTCTGCCAACGCCATTTACTCTCTGGCTGCCAG; GCCTCTTCCTATGCCAAAACTGCCACCTGGGGAGCAAGGGGAAAGTGAAGAGGACACAGAATATATGACT; CCCACATCTAGGCCTGTAGGGGTTCAGAAGCCAGAGCCCAAACGGCCGTTAGAGGCAACCCAGAGTTCAC; GAGCATGTGACTGTGACCAGCAGATCGACAGCTGTACCTATGAAGCGATGTATAACATCCAGTCCCAAGC; ACTGTCTGTAGCAGAAAACAGCGCCTCTGGGGAAGGGAATCTGGCCACAGCTCACACGAGTACTGGCCCT; GAGGAATCCGAAAACGAGGATGATGGCTATGATGTGCCTAAGCCACCCGTGCCAGCTGTACTGGCCCGCC; GGACCCTGTCTGACATCTCCAATGCCAGCTCCTCCTTTGGCTGGTTGTCTTTGGATGGTGACCCTACAAA; CTTCAATGAGGGTTCCCAAGTTCCTGAGCGGCCCCCCAAACCATTCCCTCGGAGAATCAACTCAGAACGA; AAAGCCAGTAGCTATCAACAAGGCGGAGGTGCCACTGCTAACCCTGTGGCCACAGCACCCTCACCGCAGC; TCTCAAGTGAGATTGAACGCCTCATGAGTCAGGGCTATTCCTACCAGGACATTCAGAAAGCTTTGGTCAT; TGCCCACAACAACATTGAGATGGCTAAAAACATCCTCCGGGAATTTGTTTCTATTTCTTCTCCTGCTCAC; GTAGCCACC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227679 representing NM_007619; Red=Cloning site Green=Tags(s); ; MAGNVKKSSGAGGGGSGGSGAGGLIGLMKDAFQPHHHHHHLSPHPPCTVDKKMVEKCWKLMDKVVRLCQN; PKLALKNSPPYILDLLPDTYQHLRTVLSRYEGKMETLGENEYFRVFMENLMKKTKQTISLFKEGKERMYE; ENSQPRRNLTKLSLIFSHMLAELKGIFPSGLFQGDTFRITKADAAEFWRKAFGEKTIVPWKSFRQALHEV; HPISSGLEAMALKSTIDLTCNDYISVFEFDIFTRLFQPWSSLLRNWNSLAVTHPGYMAFLTYDEVKARLQ; KFIHKPGSYIFRLSCTRLGQWAIGYVTADGNILQTIPHNKPLFQALIDGFREGFYLFPDGRNQNPDLTGL; CEPTPQDHIKVTQEQYELYCEMGSTFQLCKICAENDKDVKIEPCGHLMCTSCLTSWQESEGQGCPFCRCE; IKGTEPIVVDPFDPRGSGSLLRQGAEGAPSPNYDDDDDERADDSLFMMKELAGAKVERPSSPFSMAPQAS; LPPVPPRLDLLQQRAPVPASTSVLGTASKAASGSLHKDKPLPIPPTLRDLPPPPPPDRPYSVGAETRPQR; RPLPCTPGDCPSRDKLPPVPSSRPGDSWLSRPIPKVPVATPNPGDPWNGRELTNRHSLPFSLPSQMEPRA; DVPRLGSTFSLDTSMTMNSSPVAGPESEHPKIKPSSSANAIYSLAARPLPMPKLPPGEQGESEEDTEYMT; PTSRPVGVQKPEPKRPLEATQSSRACDCDQQIDSCTYEAMYNIQSQALSVAENSASGEGNLATAHTSTGP; EESENEDDGYDVPKPPVPAVLARRTLSDISNASSSFGWLSLDGDPTNFNEGSQVPERPPKPFPRRINSER; KASSYQQGGGATANPVATAPSPQLSSEIERLMSQGYSYQDIQKALVIAHNNIEMAKNILREFVSISSPAH; VAT; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007619
• ORF Size: 2739 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_007619.2, NP_031645.2
• RefSeq Size: 5083 bp
• RefSeq ORF: 2742 bp
• Locus ID: 12402
• UniProt ID: P22682
• Cytogenetics: 9 24.72 cM
• MW: 101 kDa
• Summary: Adapter protein that functions as a negative regulator of many signaling pathways that are triggered by activation of cell surface receptors. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including KIT, FLT1, FGFR1, FGFR2, PDGFRA, PDGFRB, EGFR, CSF1R, EPHA8 and KDR and terminates signaling. Recognizes membrane-bound HCK, SRC and other kinases of the SRC family and mediates their ubiquitination and degradation. Participates in signal transduction in hematopoietic cells. Plays an important role in the regulation of osteoblast differentiation and apoptosis. Essential for osteoclastic bone resorption. The 'Tyr-737' phosphorylated form induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3 (PubMed:10393178, PubMed:12649282, PubMed:19265199, PubMed:20100865, PubMed:9653117). In association with CBLB, required for proper feedback inhibition of ciliary platelet-derived growth factor receptor-alpha (PDGFRA) signaling pathway via ubiquitination and internalization of PDGFRA (PubMed:29237719).[UniProtKB/Swiss-Prot Function]