Tlr2 (NM_011905) Mouse Tagged ORF Clone

SKU MR227674

Tlr2 (Myc-DDK-tagged) - Mouse toll-like receptor 2 (Tlr2)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Tlr2
• Synonyms: Ly105
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227674 representing NM_011905; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGCTACGAGCTCTTTGGCTCTTCTGGATCTTGGTGGCCATAACAGTCCTCTTCAGCAAACGCTGTTCTG; CTCAGGAGTCTCTGTCATGTGATGCTTCTGGGGTGTGTGATGGCCGCTCCAGGTCTTTCACCTCTATTCC; CTCCGGACTCACAGCAGCCATGAAAAGCCTTGACCTGTCTTTCAACAAGATCACCTACATTGGCCATGGT; GACCTCCGAGCGTGTGCGAACCTCCAGGTTCTGATGTTGAAGTCCAGCAGAATCAATACAATAGAGGGAG; ACGCCTTTTATTCTCTGGGCAGTCTTGAACATTTGGATTTGTCTGATAATCACCTATCTAGTTTATCTTC; CTCCTGGTTCGGGCCCCTTTCCTCTTTGAAATACTTAAACTTAATGGGAAATCCTTACCAGACACTGGGG; GTAACATCGCTTTTTCCCAATCTCACAAATTTACAAACCCTCAGGATAGGAAATGTAGAGACTTTCAGTG; AGATAAGGAGAATAGATTTTGCTGGGCTGACTTCTCTCAATGAACTTGAAATTAAGGCATTAAGTCTCCG; GAATTATCAGTCCCAAAGTCTAAAGTCGATCCGCGACATCCATCACCTGACTCTTCACTTAAGCGAGTCT; GCTTTCCTGCTGGAGATTTTTGCAGATATTCTGAGTTCTGTGAGATATTTAGAACTAAGAGATACTAACT; TGGCCAGGTTCCAGTTTTCACCACTGCCCGTAGATGAAGTCAGCTCACCGATGAAGAAGCTGGCATTCCG; AGGCTCGGTTCTCACTGATGAAAGCTTTAACGAGCTCCTGAAGCTGTTGCGTTACATCTTGGAACTGTCG; GAGGTAGAGTTCGACGACTGTACCCTCAATGGGCTCGGCGATTTCAACCCCTCGGAGTCAGACGTAGTGA; GCGAGCTGGGTAAAGTAGAAACAGTCACTATCCGGAGGTTGCATATCCCCCAGTTCTATTTGTTTTATGA; CCTGAGTACTGTCTATTCCCTCCTGGAGAAGGTGAAGCGAATCACAGTAGAGAACAGCAAGGTCTTCCTG; GTTCCCTGCTCGTTCTCCCAGCATTTAAAATCATTAGAATTCTTAGACCTCAGCGAAAATCTGATGGTTG; AAGAATATTTGAAGAACTCAGCCTGTAAGGGAGCCTGGCCTTCTCTACAAACCTTAGTTTTGAGCCAGAA; TCATTTGAGATCAATGCAAAAAACAGGAGAGATTTTGCTGACTCTGAAAAACCTGACCTCTCTTGACATC; AGCAGGAACACTTTTCATCCGATGCCCGACAGCTGTCAGTGGCCAGAAAAGATGCGCTTCCTGAATTTGT; CCAGTACAGGGATCCGGGTGGTAAAAACGTGCATTCCTCAGACGCTGGAGGTGTTGGATGTTAGTAACAA; CAATCTTGACTCATTTTCTTTGTTCTTGCCTCGGCTGCAAGAGCTCTATATTTCCAGAAATAAGCTGAAA; ACACTCCCAGATGCTTCGTTGTTCCCTGTGTTGCTGGTCATGAAAATCAGAGAGAATGCAGTAAGTACTT; TCTCTAAAGACCAACTTGGTTCTTTTCCCAAACTGGAGACTCTGGAAGCAGGCGACAACCACTTTGTTTG; CTCCTGCGAACTCCTATCCTTTACTATGGAGACGCCAGCTCTGGCTCAAATCCTGGTTGACTGGCCAGAC; AGCTACCTGTGTGACTCTCCGCCTCGCCTGCACGGCCACAGGCTTCAGGATGCCCGGCCCTCCGTCTTGG; AATGTCACCAGGCTGCACTGGTGTCTGGAGTCTGCTGTGCCCTTCTCCTGTTGATCTTGCTCGTAGGTGC; CCTGTGCCACCATTTCCACGGACTGTGGTACCTGAGAATGATGTGGGCGTGGCTCCAGGCCAAGAGGAAG; CCCAAGAAAGCTCCCTGCAGGGACGTTTGCTATGATGCCTTTGTTTCCTACAGTGAGCAGGATTCCCATT; GGGTGGAGAACCTCATGGTCCAGCAGCTGGAGAACTCTGACCCGCCCTTTAAGCTGTGTCTCCACAAGCG; GGACTTCGTTCCGGGCAAATGGATCATTGACAACATCATCGATTCCATCGAAAAGAGCCACAAAACTGTG; TTCGTGCTTTCTGAGAACTTCGTACGGAGCGAGTGGTGCAAGTACGAACTGGACTTCTCCCACTTCAGGC; TCTTTGACGAGAACAACGACGCGGCCATCCTTGTTTTGCTGGAGCCCATTGAGAGGAAAGCCATTCCCCA; GCGCTTCTGCAAACTGCGCAAGATAATGAACACCAAGACCTACCTGGAGTGGCCCTTGGATGAAGGCCAG; CAGGAAGTGTTTTGGGTAAATCTGAGAACTGCAATAAAGTCC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227674 representing NM_011905; Red=Cloning site Green=Tags(s); ; MLRALWLFWILVAITVLFSKRCSAQESLSCDASGVCDGRSRSFTSIPSGLTAAMKSLDLSFNKITYIGHG; DLRACANLQVLMLKSSRINTIEGDAFYSLGSLEHLDLSDNHLSSLSSSWFGPLSSLKYLNLMGNPYQTLG; VTSLFPNLTNLQTLRIGNVETFSEIRRIDFAGLTSLNELEIKALSLRNYQSQSLKSIRDIHHLTLHLSES; AFLLEIFADILSSVRYLELRDTNLARFQFSPLPVDEVSSPMKKLAFRGSVLTDESFNELLKLLRYILELS; EVEFDDCTLNGLGDFNPSESDVVSELGKVETVTIRRLHIPQFYLFYDLSTVYSLLEKVKRITVENSKVFL; VPCSFSQHLKSLEFLDLSENLMVEEYLKNSACKGAWPSLQTLVLSQNHLRSMQKTGEILLTLKNLTSLDI; SRNTFHPMPDSCQWPEKMRFLNLSSTGIRVVKTCIPQTLEVLDVSNNNLDSFSLFLPRLQELYISRNKLK; TLPDASLFPVLLVMKIRENAVSTFSKDQLGSFPKLETLEAGDNHFVCSCELLSFTMETPALAQILVDWPD; SYLCDSPPRLHGHRLQDARPSVLECHQAALVSGVCCALLLLILLVGALCHHFHGLWYLRMMWAWLQAKRK; PKKAPCRDVCYDAFVSYSEQDSHWVENLMVQQLENSDPPFKLCLHKRDFVPGKWIIDNIIDSIEKSHKTV; FVLSENFVRSEWCKYELDFSHFRLFDENNDAAILVLLEPIERKAIPQRFCKLRKIMNTKTYLEWPLDEGQ; QEVFWVNLRTAIKS; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011905
• ORF Size: 2352 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_011905.3, NP_036035.3
• RefSeq Size: 2874 bp
• RefSeq ORF: 2355 bp
• Locus ID: 24088
• UniProt ID: Q9QUN7
• Cytogenetics: 3 E3
• MW: 89.9 kDa
• Summary: Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (By similarity) (PubMed:15690042). May also promote apoptosis in response to lipoproteins (By similarity). Forms activation clusters composed of several receptors depending on the ligand, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Forms the cluster TLR2:TLR6:CD14:CD36 in response to diacylated lipopeptides and TLR2:TLR1:CD14 in response to triacylated lipopeptides (By similarity). Recognizes M.tuberculosis major T-antigen EsxA (ESAT-6) which inhibits downstream MYD88-dependent signaling (PubMed:17486091). Acts as the major receptor for M.tuberculosis lipoproteins LprA, LprG, LpqH and PhoS1 (pstS1), in conjunction with TLR1 and for some but not all lipoproteins CD14 and/or CD36. The lipoproteins act as agonists to modulate antigen presenting cell functions in response to the pathogen (PubMed:19362712). Recombinant MPT83 from M.tuberculosis stimulates secretion of cytokines (TNF-alpha, IL-6 and IL-12p40) by mouse macrophage cell lines in a TLR2-dependent fashion, which leads to increased host innate immunity responses against the bacterium (PubMed:22174456). Lung macrophages which express low levels of TLR2 respond poorly to stimulation by M.tuberculosis LpqH (PubMed:19362712). Required for normal uptake of M.tuberculosis, a process that is inhibited by M.tuberculosis LppM (PubMed:27220037). Interacts with TICAM2 (By similarity).[UniProtKB/Swiss-Prot Function]