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Egr2 (NM_010118) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR227610
Egr2 (Myc-DDK-tagged) - Mouse early growth response 2 (Egr2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $686.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Egr2
Synonyms Egr-2; Krox-20; Krox20; NGF1-B; Zfp-6; Zfp-25
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR227610 representing NM_010118
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGACCGCCAAGGCCGTAGACAAAATCCCAGTAACTCTCAGTGGTTTTATGCACCAGCTGCCTGACA
GCCTCTACCCGGTGGAAGACCTCGCCGCCTCGTCGGTGACCATCTTCCCCAATGGTGAACTGGGAGGCCC
CTTTGACCAGATGAACGGAGTGGCGGGAGATGGCATGATCAACATTGACATGACTGGAGAGAAGAGACCC
CTGGATCTCCCGTATCCGAGTAGCTTCGCTCCCATCTCTGCACCTAGAAACCAGACCTTCACTTACATGG
GCAAATTCTCCATTGACCCACAGTACCCTGGTGCCAGCTGCTATCCAGAAGGTATCATCAATATTGTGAG
TGCGGGCATCTTGCAAGGGGTCACCCCTCCAGCTTCAACCACAGCCTCCTCCAGCGTCACCTCCGCCTCC
CCCAACCCACTGGCCACGGGACCCCTGGGTGTGTGTACCATGTCCCAGACTCAGCCTGAACTGGACCACC
TCTACTCTCCGCCACCACCTCCTCCTCCTTATTCGGGCTGTACAGGAGATCTCTACCAGGATCCTTCAGC
ATTCTTATCGCCGCCATCCACCACTTCCACCTCCTCTCTGGCCTACCAGCCACCTCCTTCCTACCCATCC
CCCAAGCCAGCTATGGACCCAGGTCTCATTCCTATGATCCCAGACTATCCTGGATTTTTTCCATCTCCGT
GCCAGAGAGATCCACACGGTGCTGCTGGCCCAGATCGAAAGCCGTTTCCCTGTCCTCTGGACTCCCTGCG
AGTGCCCCCTCCACTCACGCCACTCTCTACCATCCGTAATTTTACTCTGGGGGGTCCCGGTGCTGGAGTC
ACGGGACCAGGAGCAAGTGGAGGTGGTGAGGGACCTCGGCTGCCTGGCAGTGGGTCTGCAGCAGTGACTG
CCACCCCTTATAATCCGCACCACCTGCCATTGCGGCCCATCCTGCGACCTCGAAAGTACCCTAACAGGCC
CAGCAAAACGCCAGTGCACGAAAGGCCCTATCCCTGCCCAGCAGAAGGTTGTGATAGGAGGTTCTCACGC
TCTGATGAGCTGACCAGGCACATCCGAATCCACACGGGCCACAAGCCCTTCCAGTGTCGGATCTGCATGC
GAAACTTCAGCCGAAGTGACCACCTTACTACTCACATCCGAACCCACACCGGGGAGAAGCCCTTTGCCTG
TGACTATTGTGGCCGCAAGTTTGCCAGGAGTGACGAAAGGAAGCGCCACACCAAGATCCACCTTCGGCAG
AAGGAACGGAAGAGCAGTGCTCCCTCTGCACCTCCATCTGCCCAGTCTTCAGCCTCTGGTCCTGGGGGCT
CGCAGGCCGGGGGCAGCCTGTGCGGTAACAGCGCCATTGGAGGACCACTGGCCTCCTGCACCTCTCGAAC
CAGGACACCG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR227610 representing NM_010118
Red=Cloning site Green=Tags(s)

MMTAKAVDKIPVTLSGFMHQLPDSLYPVEDLAASSVTIFPNGELGGPFDQMNGVAGDGMINIDMTGEKRP
LDLPYPSSFAPISAPRNQTFTYMGKFSIDPQYPGASCYPEGIINIVSAGILQGVTPPASTTASSSVTSAS
PNPLATGPLGVCTMSQTQPELDHLYSPPPPPPPYSGCTGDLYQDPSAFLSPPSTTSTSSLAYQPPPSYPS
PKPAMDPGLIPMIPDYPGFFPSPCQRDPHGAAGPDRKPFPCPLDSLRVPPPLTPLSTIRNFTLGGPGAGV
TGPGASGGGEGPRLPGSGSAAVTATPYNPHHLPLRPILRPRKYPNRPSKTPVHERPYPCPAEGCDRRFSR
SDELTRHIRIHTGHKPFQCRICMRNFSRSDHLTTHIRTHTGEKPFACDYCGRKFARSDERKRHTKIHLRQ
KERKSSAPSAPPSAQSSASGPGGSQAGGSLCGNSAIGGPLASCTSRTRTP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_010118
ORF Size 1410 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010118.3, NP_034248.2
RefSeq Size 2972 bp
RefSeq ORF 1413 bp
Locus ID 13654
UniProt ID P08152
Cytogenetics 10 34.96 cM
MW 50.3 kDa
Summary Sequence-specific DNA-binding transcription factor. Binds to two specific DNA sites located in the promoter region of HOXA4. Binds to the promoter region of ERBB2. May play a role in the regulation of hindbrain segmentation, might act in combination with the Hox network to specify odd and even rhombomeres, and might participate in the control of the expression of some of the homeobox containing genes.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_010118" in other vectors (6)
SKU Description Size Price
MC216445 Egr2 (untagged) - Mouse early growth response 2 (Egr2), (10ug) 10 ug
$503.00
MG227610 Egr2 (tGFP-tagged) - Mouse early growth response 2 (Egr2), (10ug) 10 ug
$886.00
MR227610L1 Lenti ORF clone of Egr2 (Myc-DDK-tagged) - Mouse early growth response 2 (Egr2) 10 ug
$986.00
MR227610L2 Lenti ORF clone of Egr2 (mGFP-tagged) - Mouse early growth response 2 (Egr2) 10 ug
$986.00
MR227610L3 Lenti ORF clone of Egr2 (Myc-DDK-tagged) - Mouse early growth response 2 (Egr2) 10 ug
$986.00
MR227610L4 Lenti ORF clone of Egr2 (mGFP-tagged) - Mouse early growth response 2 (Egr2) 10 ug
$986.00

Plasmid Map for MR227610

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR227610
Circular map for MR227610

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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