Reck (NM_016678) Mouse Tagged ORF Clone

SKU MR227392

Reck (Myc-DDK-tagged) - Mouse reversion-inducing-cysteine-rich protein with kazal motifs (Reck)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Reck
• Synonyms: mRECK; St15
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227392 representing NM_016678; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCGAGCGTCCGGGCCTCCCCGCGCAGCGCGCTGCTACTTCTGCTGGCCGCGGCGGGGGTCGCGGAGG; TGACGGGGGGCCTGGCCCCGGGCAGCGCGGGTGCTGTATGCTGTAATCATTCAAAAGATAACCAAATGTG; CCGTGATGTATGTGAGCAGATTTTCTCCTCAAAAAGTGAATCCCGACTGAAACATCTGTTGCAGAGAGCC; CCAGATTATTGCCCTGAAACAATGGTTGAAATTTGGAGTTGCATGAATTCGTCTTTGCCAGGGGTATTTA; AGAAGTCCGATGGTTGGGTTGGCTTAGGCTGCTGTGAACTGGCTATTGGCTTGGAGTGCCGACAGGCATG; CAAGCAGGCATCTTCGAAAAATGATATTTCCAAAGTTTGCAGGAAAGAATATGAGAATGCTCTTTTCAGT; TGCATTAGCAGAAATGAAATGGGCTCGGTGTGCTGCAGTTACGCAGGCCATCACACCAACTGCCGAGAAT; TCTGTCAAGCCATTTTTCGAACAGACTCTTCTCCTGGGCCATCTCAGATCAAAGCAGTGGAAAATTATTG; TGCCTCTATCAGTCCACAGTTGATACACTGTGTGAATAATTACACCCAGTCTTATCCAATGAGAAACCCA; ACTGATAGTTTATATTGCTGTGACAGAGCTGAAGACCATGCTTGCCAAAACGCCTGCAAGAGAATTCTAA; TGTCTAAGAAAACAGAAATGGAGATTGTTGATGGTCTCATTGAGGGTTGTAAGACCCAGCCCTTGCCTCA; AGATCCTCTTTGGCAGTGTTTTCTCGAAAGCTCACAGTCGGTTCACCCTGGAGTCACTGTGCACCCTCCT; CCCTCAACTGGCCTCGACGGGGCCAAATTGCACTGTTGCTCTAAAGCAAACACTTCAACCTGTAGGGAAC; TGTGCACCAAACTTTATAGCATGAGCTGGGGAAATACACAGAGTTGGCAAGAGTTTGACCGCATCTGTGA; GTATAATCCGGTGGAAGTGTCCATGCTGACGTGTCTGGCTGATGTCCGGGAGCCTTGCCAGTTGGGCTGT; ACAAACCTGACTTACTGTACAAACTTTAACAACAGGCCAACAGAACTGTTCAGGAGCTGCACGGCTCAGT; CTGACCAGGGGGCCATGAGTGACATGAAGCTGTGGGAGAAGGGGAGCATCAAGATGCCCTTCATCAGCAT; CCCTGTCCTAGACATCAAGACCTGCCAGCCAGAGATGTGGAAGGCCGTGGCCTGCTCCCTGCAGATCAAG; CCGTGCCATAGCAAATCCCGAGGCAGTATTATCTGCAAATCAGATTGTGTAGAGATTCTCAAGAAATGTG; GAGACCAGAATAAATTCCCTGAAGAACACACAGCTGAAAGTATCTGTGAGTTTCTGTCCCCTGCAGATGA; CCTGGAGAGCTGTATCCCTCTGGACACGTACCTCAGGCCAAGTGCTTTAGGAAACATCATAGAAGAAGTG; ACTCACCCCTGTAACCCAAACCCCTGCCCAGCCAACGAGCTCTGTGAAGTGAACCGGAAGGGGTGTCCGT; CTGCAGATCCCTGCCTTCCATACTCCTGTGTTCAAGGCTGCAAATTGGGAGAAGCTTCTGACTTCATCGT; CCGCCAGGGGACACTGATCCAGGTGCCGTCCTCTGCAGGGGAAGTTGGTTGCTACAAGATCTGTTCCTGT; GGACAAAGTGGCCTCTTGGAAAACTGTATGGAAATGCACTGTATCGACCTCCAGAAGTCTTGTATTGTCG; GAGGAAAAAGAAAAAGCCACGGAACATCCTTTACTATTGACTGCAATGTCTGTTCTTGTTTTGCGGGTAA; TTTAGTGTGTTCCACCCGCCTGTGCCTCAGTGAGCACAGTTCAGACGACGACCGCCGCACCTTCACAGGT; CTGCCCTGTAACTGCGCAGATCAGTTTGTCCCAGTGTGTGCACAGAATGGACGCACTTACCCCAGTGCCT; GCATCGCTCGCTGTGTGGGTCTCCAGGACCATCAGTTTGAGTTTGGACCTTGTATCTCCAAGAATCCCTG; TAATCCTAACCTCTGCCCCAAAAGCCAAAGATGTGTGCCAAAACCACAAGTCTGCCTGACGACTTTTGAT; AAATTTGGATGTAGCCAATATGAGTGTGTGCCCAGACAGCTCACCTGTGACCAGGCCCGAGATCCTGTGT; GTGACACAGATCACATGGAGCACAGCAACCTCTGCACTCTGTACCAAAGAGGGAAAAGCCTGTCTTACAG; AGGCCCTTGCCAGCCTTTCTGCAGAGCCAAGGAGCCTGTCTGTGGGCACAATGGTGAGACATACAGCAGT; GTGTGTGCTGCCTACTCAGATCGTGTGGCCGTGGATTACTACGGGCCCTGCCAGGCTGTCGGCGTCCTCT; CGGAATACAGTGCTGTGGCTGAGTGTGCTGCTGTCAAGTGTCCTTCCCTCTCAGCCATAGGCTGCAAACC; CATCATCCCACCAGGGGCTTGCTGTCCACTATGTGCTGGGATGTTACGGGTATTATTTGACAAAGAAAAA; CTGGACACTATTGCTAAGGTGACAAGCAAGAAGCCCATAACCGTTGTGGAGATTCTTCAGAAGGTCCGCA; TGCACGTGTCTGTGCCGCAGTGTGACGTGTTTGGATACTTGAGCATCGAGTCGGAAATTGTGATCCTCAT; TATTCCTGTGGACCATTACCCAAAAGCTTTGCAGATTGAGGCCTGCAATAAGGAGGCAGAGAAAATCGAG; TCCCTCATCAACTCCGACAGCCCCACGCTGGCGTCCCACGTCCCACTCTCGGCCCTCATCATTTCCCAAG; TGCAGGTCTCCAGCAGTCTCCCGTCATCTGCTGTTGTGGGCAGGCCTCTCTTCCACTCCCTGCTCCTCCT; CCTCAGCCTGGGCCTCACCGTGCACTTGCTCTGGACTAGACCC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227392 representing NM_016678; Red=Cloning site Green=Tags(s); ; MASVRASPRSALLLLLAAAGVAEVTGGLAPGSAGAVCCNHSKDNQMCRDVCEQIFSSKSESRLKHLLQRA; PDYCPETMVEIWSCMNSSLPGVFKKSDGWVGLGCCELAIGLECRQACKQASSKNDISKVCRKEYENALFS; CISRNEMGSVCCSYAGHHTNCREFCQAIFRTDSSPGPSQIKAVENYCASISPQLIHCVNNYTQSYPMRNP; TDSLYCCDRAEDHACQNACKRILMSKKTEMEIVDGLIEGCKTQPLPQDPLWQCFLESSQSVHPGVTVHPP; PSTGLDGAKLHCCSKANTSTCRELCTKLYSMSWGNTQSWQEFDRICEYNPVEVSMLTCLADVREPCQLGC; TNLTYCTNFNNRPTELFRSCTAQSDQGAMSDMKLWEKGSIKMPFISIPVLDIKTCQPEMWKAVACSLQIK; PCHSKSRGSIICKSDCVEILKKCGDQNKFPEEHTAESICEFLSPADDLESCIPLDTYLRPSALGNIIEEV; THPCNPNPCPANELCEVNRKGCPSADPCLPYSCVQGCKLGEASDFIVRQGTLIQVPSSAGEVGCYKICSC; GQSGLLENCMEMHCIDLQKSCIVGGKRKSHGTSFTIDCNVCSCFAGNLVCSTRLCLSEHSSDDDRRTFTG; LPCNCADQFVPVCAQNGRTYPSACIARCVGLQDHQFEFGPCISKNPCNPNLCPKSQRCVPKPQVCLTTFD; KFGCSQYECVPRQLTCDQARDPVCDTDHMEHSNLCTLYQRGKSLSYRGPCQPFCRAKEPVCGHNGETYSS; VCAAYSDRVAVDYYGPCQAVGVLSEYSAVAECAAVKCPSLSAIGCKPIIPPGACCPLCAGMLRVLFDKEK; LDTIAKVTSKKPITVVEILQKVRMHVSVPQCDVFGYLSIESEIVILIIPVDHYPKALQIEACNKEAEKIE; SLINSDSPTLASHVPLSALIISQVQVSSSLPSSAVVGRPLFHSLLLLLSLGLTVHLLWTRP; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016678
• ORF Size: 2913 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_016678.2, NP_057887.2
• RefSeq Size: 4450 bp
• RefSeq ORF: 2916 bp
• Locus ID: 53614
• UniProt ID: Q9Z0J1
• Cytogenetics: 4 B1
• MW: 106.5 kDa
• Summary: Functions together with ADGRA2 to enable brain endothelial cells to selectively respond to Wnt7 signals (WNT7A or WNT7B) (PubMed:28803732). Plays a key role in Wnt7-specific responses: required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation (PubMed:26658478, PubMed:28803732). Acts as a Wnt7-specific coactivator of canonical Wnt signaling by decoding Wnt ligands: acts by interacting specifically with the disordered linker region of Wnt7, thereby conferring ligand selectivity for Wnt7 (By similarity). ADGRA2 is then required to deliver RECK-bound Wnt7 to frizzled by assembling a higher-order RECK-ADGRA2-Fzd-LRP5-LRP6 complex (By similarity). Also acts as a serine protease inhibitor: negatively regulates matrix metalloproteinase-9 (MMP9) by suppressing MMP9 secretion and by direct inhibition of its enzymatic activity (PubMed:11747814). Also inhibits metalloproteinase activity of MMP2 and MMP14 (MT1-MMP) (PubMed:11747814).[UniProtKB/Swiss-Prot Function]