Itga6 (NM_008397) Mouse Tagged ORF Clone
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Itga6 |
| Synonyms | 5033401O05Rik; AI115430; Cd49f; VLA-6 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
![]() Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_008397 |
| ORF Size | 3219 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_008397.4, NP_032423.2 |
| RefSeq Size | 4205 bp |
| RefSeq ORF | 3222 bp |
| Locus ID | 16403 |
| Cytogenetics | 2 42.79 cM |
| MW | 120.1 kDa |
| Summary | This gene encodes a protein that is a member of the integrin superfamily. Integrins are transmembrane receptors involved cell adhesion and signaling, and they are subdivided based on the heterodimer formation of alpha and beta chains. This protein has been shown to heterodimerize with beta 4 to bind laminin and to form the main component of hemidesmosomes, which mediate attachment of epithelia to basement membranes. In mouse, deficiency of this gene is associated with absence of hemidesmosomes, severe skin blistering, and early post-natal death. In humans mutations of this gene are associated with epidermolysis bullosa. Alternative splicing results in multiple transcript variants that encode different protein isoforms. provided by RefSeq, May 2013 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_008397" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC223447 | Itga6 (untagged) - Mouse integrin alpha 6 (Itga6), (10ug) | 10 ug |
$1,028.00
|
|
| MG227346 | Itga6 (tGFP-tagged) - Mouse integrin alpha 6 (Itga6), (10ug) | 10 ug |
$789.00
$1,134.00
|
|
| MR227346L3 | Lenti ORF clone of Itga6 (Myc-DDK-tagged) - Mouse integrin alpha 6 (Itga6) | 10 ug |
$1,234.00
|
|
| MR227346L4 | Lenti ORF clone of Itga6 (mGFP-tagged) - Mouse integrin alpha 6 (Itga6) | 10 ug |
$1,234.00
|
|
Citations
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