Cdh3 (NM_007665) Mouse Tagged ORF Clone
$589.00
$752.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Cdh3 |
| Synonyms | AI385538; Ca; Cadp; Cdhp; P-cad; P-cadherin; Pc; Pcad |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_007665 |
| ORF Size | 2463 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_007665.3, NP_031691.1 |
| RefSeq Size | 4034 bp |
| RefSeq ORF | 2466 bp |
| Locus ID | 12560 |
| Cytogenetics | 8 53.16 cM |
| MW | 91 kDa |
| Summary | This gene encodes a calcium-dependent cell-cell adhesion protein containing five cadherin domains. The encoded protein plays a role in epithelial outgrowth, such as that which occurs during the development of hair follicles and limb buds. Loss of function of the related gene in humans results in ectodermal dysplasia, ectrodactyly, and macular dystrophy and congential hypotrichosis with juvenile macular dystrophy. This gene is located in the vicinity of similar cadherin genes on chromosome 8. The proprotein is further cleaved into a functional chain. Alternative splicing results in multiple transcript variants. provided by RefSeq, Mar 2013 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_007665" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC221847 | Cdh3 (untagged) - Mouse cadherin 3 (Cdh3), transcript variant 2, (10ug) | 10 ug |
$828.00
|
|
| MG227345 | Cdh3 (tGFP-tagged) - Mouse cadherin 3 (Cdh3) transcript variant 2, (10ug) | 10 ug |
$952.00
|
|
| MR227345L3 | Lenti ORF clone of Cdh3 (Myc-DDK-tagged) - Mouse cadherin 3 (Cdh3), transcript variant 2 | 10 ug |
$1,052.00
|
|
| MR227345L4 | Lenti ORF clone of Cdh3 (mGFP-tagged) - Mouse cadherin 3 (Cdh3), transcript variant 2 | 10 ug |
$1,052.00
|
|
Citations
Stem cell-derived synthetic embryos self-assemble by exploiting cadherin codes and cortical tension
Bao, Cornwall-Scoones, Sanchez-Vasquez et al
Nat Cell Biol (2022) 24 (9), 1341-1349 DOI: 10.1038/s41556-022-00984-y
Bao, Cornwall-Scoones, Sanchez-Vasquez et al
Nat Cell Biol (2022) 24 (9), 1341-1349 DOI: 10.1038/s41556-022-00984-y
Cited by 92
AI Summary: OriGene products, specifically the Myc-DDK tagged Cdh3 (MR227345) and mouse-tagged ORF clone Cdh6 (MG222740), were used as sources for cadherin gene constructs to study the role of cadherin codes in stem cell sorting and synthetic embryo assembly in this research.
Snippet: er Scientific). PiggyBac-based expression plasmids for Cdh1, Cdh3 or Cdh6 were generated by PCR amplification of the respective genes in pENTR-Cdh1 (49776;Addgene), Cdh3 (Myc-DDK tagged) (MR227345;OriGene Technologies) or Cdh6 (mouse-tagged ORF clone) (MG222740;OriGene Technologies) with the oligos listed in Supplementary Table 1. Small interfering RNA Cells were transfected with 25 nM smal
Showing 1-1 of 1 papers.
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