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P2rx7 (NM_011027) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR227216
P2rx7 (Myc-DDK-tagged) - Mouse purinergic receptor P2X, ligand-gated ion channel, 7 (P2rx7), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $554.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol P2rx7
Synonyms AI467586; P2X(7); P2X7R
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR227216 representing NM_011027
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGGCTTGCTGCAGCTGGAACGATGTCTTGCAGTATGAGACAAACAAAGTCACCCGGATCCAGAGCA
CGAATTATGGCACCGTCAAGTGGGTCTTGCACATGATCGTCTTTTCCTACATTAGCTTTGCTTTGGTGAG
CGATAAGCTGTACCAGCGGAAAGAGCCTGTTATCAGCTCCGTGCACACCAAGGTCAAAGGCATAGCAGAG
GTGACGGAGAATGTCACAGAGGGTGGGGTGACGAAGTTAGGACACAGCATCTTTGACACTGCAGACTACA
CCTTCCCTTTGCAGGGGAACTCATTCTTTGTCATGACAAACTATGTCAAGTCAGAAGGCCAAGTGCAGAC
GCTGTGTCCTGAGTATCCCAGGCGCGGTGCACAGTGCTCTTCTGACCGGCGTTGTAAAAAGGGGTGGATG
GACCCACAGAGCAAAGGAATCCAGACTGGCAGGTGTGTTCCATATGACAAGACAAGGAAGACCTGTGAAG
TCTCTGCCTGGTGTCCTACTGAGGAGGAGAAAGAAGCTCCCCGACCTGCACTCTTGAGGAGCGCCGAAAA
CTTCACCGTACTCATCAAGAATAATATCCACTTCCCCGGCCACAACTATACCACGAGAAACATCTTGCCA
ACTATGAACGGCTCTTGTACCTTTCACAAGACTTGGGACCCTCAGTGTTCCATCTTCCGACTAGGGGACA
TCTTCCAGGAAGCAGGAGAGAACTTTACAGAGGTGGCAGTTCAGGGAGGAATCATGGGTATCGAGATCTA
CTGGGATTGCAACCTAGACAGCTGGTCCCATCACTGCCGACCCAGGTACAGCTTCCGCCGCCTGGATGAC
AAGAACACGGATGAGTCCTTCGTCCCCGGCTACAACTTCAGATATGCCAAGTACTATAAGGAGAACAATG
TGGAAAAGCGGACGTTGATCAAAGCCTTCGGCATCCGTTTTGACATCCTGGTTTTCGGCACTGGAGGAAA
ATTTGACATCATCCAGCTGGTTGTATACATTGGATCCACCCTGTCCTACTTTGGCTTGGCCACTGTGTGC
ATTGACTTGCTCATCAACACATACTCCAGTGCTTTCTGCAGGTCGGGGGTTTACCCCTACTGTAAGTGCT
GTGAGCCCTGCACAGTGAACGAGTATTACTACAGAAAGAAGTGTGAGTCCATCATGGAACCCAAGCCGAC
GTTGAAGTATGTGTCCTTTGTCGACGAGCCGCACATTCGCATGGTGGACCAGCAGCTGCTTGGGAAAAGT
CTGCAAGTTGTCAAAGGCCAAGAAGTTCCAAGACCCCAGATGGACTTCTCCGACCTGTCTAGGCTGTCCC
TATCTCTCCACGACTCACCCCTGACTCCTGGACAATCTGAGGAAATTCAGCTGCTCCATGAAGAGGTGGC
CCCTAAGTCCGGGGACAGCCCGAGTTGGTGCCAGTGTGGAAATTGCCTCCCGTCTCGCCTACCGGAGCAA
CGCAGGGCCCTGGAGGAGCTGTGCTGCCGGAGGAAGCCGGGGCGGTGCATCACCACCTCCAAGCTCTTCC
ATAAGCTCGTGCTGTCCCGAGACACCCTGCAGCTCCTCCTGCTTTACCAGGATCCCTTGCTGGTGCTGGG
GGAAGAGGCCACCAACAGCAGGCTGCGACACCGTGCTTACAGGTGCTATGCCACCTGGCGCTTCGGCTCC
CAGGACATGGCCGACTTTGCCATTCTGCCCAGCTGTTGCCGCTGGAGGATCCGGAAGGAGTTCCCCAAGA
CCGAGGGGCAGTATAGTGGCTTCAAGTATCCCTAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR227216 representing NM_011027
Red=Cloning site Green=Tags(s)

MPACCSWNDVLQYETNKVTRIQSTNYGTVKWVLHMIVFSYISFALVSDKLYQRKEPVISSVHTKVKGIAE
VTENVTEGGVTKLGHSIFDTADYTFPLQGNSFFVMTNYVKSEGQVQTLCPEYPRRGAQCSSDRRCKKGWM
DPQSKGIQTGRCVPYDKTRKTCEVSAWCPTEEEKEAPRPALLRSAENFTVLIKNNIHFPGHNYTTRNILP
TMNGSCTFHKTWDPQCSIFRLGDIFQEAGENFTEVAVQGGIMGIEIYWDCNLDSWSHHCRPRYSFRRLDD
KNTDESFVPGYNFRYAKYYKENNVEKRTLIKAFGIRFDILVFGTGGKFDIIQLVVYIGSTLSYFGLATVC
IDLLINTYSSAFCRSGVYPYCKCCEPCTVNEYYYRKKCESIMEPKPTLKYVSFVDEPHIRMVDQQLLGKS
LQVVKGQEVPRPQMDFSDLSRLSLSLHDSPLTPGQSEEIQLLHEEVAPKSGDSPSWCQCGNCLPSRLPEQ
RRALEELCCRRKPGRCITTSKLFHKLVLSRDTLQLLLLYQDPLLVLGEEATNSRLRHRAYRCYATWRFGS
QDMADFAILPSCCRWRIRKEFPKTEGQYSGFKYPY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_011027
ORF Size 1785 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_011027.4
RefSeq Size 4931 bp
RefSeq ORF 1788 bp
Locus ID 18439
UniProt ID Q9Z1M0
Cytogenetics 5 F
MW 68.8 kDa
Summary Receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells. In the absence of its natural ligand, ATP, functions as a scavenger receptor in the recognition and engulfment of apoptotic cells.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_011027" in other vectors (4)
SKU Description Size Price
MC219512 P2rx7 (untagged) - Mouse purinergic receptor P2X, ligand-gated ion channel, 7 (P2rx7), transcript variant 1, (10ug) 10 ug
$610.00
MG227216 P2rx7 (tGFP-tagged) - Mouse purinergic receptor P2X ligand-gated ion channel 7 (P2rx7) transcript variant 1, (10ug) 10 ug
$489.00 $754.00
MR227216L3 Lenti ORF clone of P2rx7 (Myc-DDK-tagged) - Mouse purinergic receptor P2X, ligand-gated ion channel, 7 (P2rx7), transcript variant 1 10 ug
$854.00
MR227216L4 Lenti ORF clone of P2rx7 (mGFP-tagged) - Mouse purinergic receptor P2X, ligand-gated ion channel, 7 (P2rx7), transcript variant 1 10 ug
$854.00

Plasmid Map for MR227216

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR227216
Circular map for MR227216

file-alt Citations

Extracellular histone proteins activate P2XR7 channel current
Al-Aqtash, Ross, Collier
J Gen Physiol (2023) 155 (7) DOI: 10.1085/jgp.202213317
Cited by 9
AI Summary: OriGene products contributed mouse and human P2XR7 cDNA (catalog numbers MR227216 and RC222143) used for heterologous expression to investigate P2XR7 channel activation by extracellular histone proteins in this study.
Snippet: cDNA for mouse and human P2XR7 was obtained from Origene (MR227216 and RC222143;Origene Technologies). Primers for mutagenesis and sequencing were
FigureFigureFigure
Showing 1-1 of 1 papers.
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