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Dnajb11 (NM_026400) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR227146
Dnajb11 (Myc-DDK-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily B, member 11 (Dnajb11), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dnajb11
Synonyms 1810031F23Rik; ABBP-2; AL024055; Dj9; ERdj3; ERj3p
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR227146 representing NM_026400
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCCGCAGAACCTGAGCACCTTCTGCCTGTTGCTGCTGTACCTCATCGGGACTGTGATCGCCGGGC
GAGATTTCTATAAGATCTTGGGGGTGCCTCGAAGTGCCTCCATAAAGGACATTAAAAAGGCCTACAGGAA
ACTGGCCCTGCAGCTCCACCCTGACCGGAACCCTGATGACCCCCAAGCCCAGGAGAAATTCCAGGATCTA
GGTGCTGCTTATGAGGTTCTGTCAGATAGTGAAAAACGAAAACAGTACGATACTTACGGTGAAGAAGGCT
TGAAAGATGGCCATCAGAGCTCTCATGGGGACATTTTTTCACACTTCTTTGGAGACTTTGGCTTCATGTT
TGGAGGAACCCCTCGTCAGCAGGACAGGAATATTCCAAGAGGAAGTGATATCATCGTAGATCTAGAAGTC
ACTCTGGAAGAAGTGTACGCAGGAAATTTTGTGGAAGTAGTTAGAAACAAGCCTGTGGCCAGGCAGGCTC
CTGGCAAACGGAAATGCAACTGTCGGCAAGAGATGAGAACCACACAGCTGGGACCGGGACGCTTCCAAAT
GACCCAGGAAGTGGTTTGTGACGAGTGCCCTAATGTCAAATTAGTGAATGAAGAACGAACACTAGAAGTG
GAAATAGAGCCTGGGGTGCGAGATGGCATGGAGTACCCCTTTATTGGAGAAGGTGAGCCGCATGTGGATG
GGGAACCCGGAGACTTACGGTTCCGAATCAAAGTTGTCAAGCACCGGATATTTGAGAGGAGAGGGGATGA
CCTGTACACAAATGTGACCGTCTCACTGGTTGAGGCTCTGGTTGGCTTTGAGATGGACATAACTCACCTG
GATGGTCACAAGGTCCATATTTCCCGGGACAAGATCACCAGGCCAGGAGCCAAGCTGTGGAAGAAAGGGG
AAGGGCTGCCCAACTTTGATAACAATAACATCAAGGGCTCTTTGATAATCACTTTTGATGTGGACTTTCC
AAAAGAACAGCTGACAGAGGAAGCAAAAGAAGGTATCAAGCAGCTTCTCAAACAAGGCCCCGTGCAGAAG
GTGTACAACGGGCTGCAGGGCTAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR227146 representing NM_026400
Red=Cloning site Green=Tags(s)

MAPQNLSTFCLLLLYLIGTVIAGRDFYKILGVPRSASIKDIKKAYRKLALQLHPDRNPDDPQAQEKFQDL
GAAYEVLSDSEKRKQYDTYGEEGLKDGHQSSHGDIFSHFFGDFGFMFGGTPRQQDRNIPRGSDIIVDLEV
TLEEVYAGNFVEVVRNKPVARQAPGKRKCNCRQEMRTTQLGPGRFQMTQEVVCDECPNVKLVNEERTLEV
EIEPGVRDGMEYPFIGEGEPHVDGEPGDLRFRIKVVKHRIFERRGDDLYTNVTVSLVEALVGFEMDITHL
DGHKVHISRDKITRPGAKLWKKGEGLPNFDNNNIKGSLIITFDVDFPKEQLTEEAKEGIKQLLKQGPVQK
VYNGLQGY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026400
ORF Size 1074 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026400.5, NP_080676.3
RefSeq Size 2534 bp
RefSeq ORF 1077 bp
Locus ID 67838
UniProt ID Q99KV1
Cytogenetics 16 B1
MW 41 kDa
Summary As a co-chaperone for HSPA5 it is required for proper folding, trafficking or degradation of proteins. Binds directly to both unfolded proteins that are substrates for ERAD and nascent unfolded peptide chains, but dissociates from the HSPA5-unfolded protein complex before folding is completed. May help recruiting HSPA5 and other chaperones to the substrate. Stimulates HSPA5 ATPase activity. It is necessary for maturation and correct trafficking of PKD1.UniProtKB/Swiss-Prot Function
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"NM_026400" in other vectors (5)
SKU Description Size Price
MC201125 Dnajb11 (untagged) - Mouse DnaJ (Hsp40) homolog, subfamily B, member 11 (Dnajb11), transcript variant 1, (10ug) 10 ug
$457.00
MG205465 Dnajb11 (tGFP-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily B, member 11 (Dnajb11) 10 ug
$657.00
MG227146 Dnajb11 (tGFP-tagged) - Mouse DnaJ (Hsp40) homolog subfamily B member 11 (Dnajb11) transcript variant 1, (10ug) 10 ug
$657.00
MR227146L3 Lenti ORF clone of Dnajb11 (Myc-DDK-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily B, member 11 (Dnajb11), transcript variant 1 10 ug
$757.00
MR227146L4 Lenti ORF clone of Dnajb11 (mGFP-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily B, member 11 (Dnajb11), transcript variant 1 10 ug
$757.00

Plasmid Map for MR227146

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR227146
Circular map for MR227146

file-alt Citations

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