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Dnajb11 (NM_026400) Mouse Untagged Clone

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SKU
MC201125
Dnajb11 (untagged) - Mouse DnaJ (Hsp40) homolog, subfamily B, member 11 (Dnajb11), transcript variant 1, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP tGFP DDK
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Dnajb11
Synonyms 1810031F23Rik; ABBP-2; AL024055; Dj9; ERdj3; ERj3p
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC018282 sequence for NM_026400
CGCTACCTGTCTCCGCGGCCGGGCAAGAGAGAGACCCCGGCGTGAGGCGGCCTCCCTGGGCCGGAAGGGC TGGTCAGCCGACGCAGCGGCACCGGAGGCTGAGAGGGTAGGACCAGGGGACGCCATGGCCCCGCAGAACC TGAGCACCTTCTGCCTGTTGCTGCTGTACCTCATCGGGACTGTGATCGCCGGGCGAGATTTCTATAAGAT CTTGGGGGTGCCTCGAAGTGCCTCCATAAAGGACATTAAAAAGGCCTACAGGAAACTAGCCCTGCAGCTC CACCCTGACCGGAACCCTGATGACCCCCAAGCCCAGGAGAAATTCCAGGATCTAGGTGCTGCTTATGAGG TTCTGTCAGATAGTGAAAAACGAAAACAGTACGATGCTTACGGTGAAGAAGGCTTGAAAGATGGCCATCA GAGCTCTCATGGGGACATTTTTTCACACTTCTTTGGAGACTTTGGCTTCATGTTTGGAGGAACCCCTCGT CAGCAGGACAGGAATATTCCAAGAGGAAGTGATATCATCGTAGATCTAGAAGTCACTCTGGAAGAAGTGT ACGCAGGAAATTTTGTGGAAGTAGTTAGAAACAAGCCTGTGGCCAGGCAGGCTCCTGGCAAACGGAAATG CAACTGTCGGCAAGAGATGAGAACCACACAGCTGGGACCGGGACGCTTCCAAATGACCCAGGAAGTGGTT TGTGACGAGTGCCCTAATGTCAAATTAGTGAATGAAGAACGAACACTAGAAGTGGAAATAGAGCCTGGGG TGCGAGATGGCATGGAGTACCCCTTTATTGGAGAAGGTGAGCCGCATGTGGATGGGGAACCCGGAGACTT ACGGTTCCGAATCAAAGTTGTCAAGCACCGGATATTTGAGAGGAGAGGGGATGACCTGTACACAAATGTG ACCGTCTCACTGGTTGAGGCTCTGGTTGGCTTTGAGATGGACATAACTCACCTGGATGGTCACAAGGTCC ATATTTCCCGGGACAAGATCACCAGGCCAGGAGCCAAGCTGTGGAAGAAAGGGGAAGGGCTGCCCAACTT TGATAACAATAACATCAAGGGCTCTTTGATAATCACTTTTGATGTGGACTTTCCAAAAGAACAGCTGACA GAGGAAGCAAAAGAAGGTATCAAGCAGCTTCTCAAACAAGGCCCCGTGCAGAAGGTGTACAACGGGCTGC AGGGCTATTAGGAGCGAAGAGAACTGGACTTTGAAAGAGGTGGATCAGCGATATTTATTATCTGCAGTGG GTTTTTGTGTGTGTGTTTCATTATTTTCAATATGCAAGTTTGGCTTAGTCCTTTTTTCCCTGATGACCGT CCTGACAAGAACTAGAGGTTTGAGGCTCTGTCTATTGCATTCAGACAACAATGGCCAGCAGAAGGTTACC TAATACCTCCCCCTCTGGGGATTTAACGTCTGGTGCTGCCCGAGGTTCAAGGAGTAACGTCTCAGAGGAT GCCCGAGCAAAGGAAACCAGATGAGGGTTAGAGACAATTGTTAGCTATTTCAAAATGCCAACTGGAGAAG TCTGTTTTTAAATACATCTTGTTGTTATTTTTTTTTTCTTGACTCCTGTTTCTTTTTCCTGTGAGCCGAT GGGAAGTCTTCCCATCAGGCAGGATGGCCACACAGCATGCAGAGGAACGATGAGAGCCCGGCTCCCAGGC CCCACTAAACACATGGTGTCTATAGCAACGAGCTCGAAGAAACCGAGTAGACTAGAGACTGAGCTACATG AATTAAAGTTACACAAAGACCTCACTGTCTTTGAGAGAACATATTTTCCCGTTTTCTTTGGTAATAATAA AATCTGATATGGGTATTTTACTGTTAAAAAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_026400
Insert Size 1077 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC018282, AAH18282
RefSeq Size 1866 bp
RefSeq ORF 1077 bp
Locus ID 67838
UniProt ID Q99KV1
Cytogenetics 16 B1
Summary As a co-chaperone for HSPA5 it is required for proper folding, trafficking or degradation of proteins. Binds directly to both unfolded proteins that are substrates for ERAD and nascent unfolded peptide chains, but dissociates from the HSPA5-unfolded protein complex before folding is completed. May help recruiting HSPA5 and other chaperones to the substrate. Stimulates HSPA5 ATPase activity. It is necessary for maturation and correct trafficking of PKD1.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same protein.
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Documents
Resources
"NM_026400" in other vectors (5)
SKU Description Size Price
MG205465 Dnajb11 (tGFP-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily B, member 11 (Dnajb11) 10 ug
$657.00
MG227146 Dnajb11 (tGFP-tagged) - Mouse DnaJ (Hsp40) homolog subfamily B member 11 (Dnajb11) transcript variant 1, (10ug) 10 ug
$657.00
MR227146 Dnajb11 (Myc-DDK-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily B, member 11 (Dnajb11), transcript variant 1 10 ug
$289.00 $457.00
MR227146L3 Lenti ORF clone of Dnajb11 (Myc-DDK-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily B, member 11 (Dnajb11), transcript variant 1 10 ug
$757.00
MR227146L4 Lenti ORF clone of Dnajb11 (mGFP-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily B, member 11 (Dnajb11), transcript variant 1 10 ug
$757.00

file-alt Citations

Erdj3 has an essential role for Z variant Alpha-1-Antitrypsin Degradation
Khodayari, Marek, Lu et al
J. Cell. Biochem. (2017)
AI Summary: In this study, the OriGene pCMV6-DNAJB11 plasmid was used to overexpress ERdj3 in AAT knockout Huh7.5 cells, enabling the authors to assess the role of ERdj3 in Z variant alpha-1-antitrypsin degradation.
Snippet: To investigate the effect of ERdj3 overexpression, AAT KO Huh7.5 cells were co-transfected with GFP tagged pCMV6-DNAJB11 (OriGene, Rockville, MD) and pTR2-ZAAT or pTR2-MAAT;48 hours post transfection, they were evaluated by native gel Western blotting ... Cite SaveSaving
Showing 1-1 of 1 papers.
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