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Htr1a (NM_008308) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR227083
Htr1a (Myc-DDK-tagged) - Mouse 5-hydroxytryptamine (serotonin) receptor 1A (Htr1a)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $686.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Htr1a
Synonyms Gpcr18
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR227083 representing NM_008308
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATATGTTCAGTCTTGGCCAGGGCAACAACACCACAACGTCCCTGGAGCCCTTCGGGACAGGCGGCA
ACGATACTGGCCTCTCCAACGTGACCTTCAGCTACCAAGTGATCACCTCTCTTTTGCTGGGGACGCTCAT
TTTCTGCGCGGTGCTCGGCAATGCCTGCGTGGTTGCTGCCATCGCCCTGGAGCGCTCCCTTCAGAATGTT
GCCAACTATCTCATCGGCTCCTTGGCGGTCACCGATCTCATGGTGTCAGTGCTGGTGCTGCCCATGGCTG
CTCTGTACCAGGTGCTCAACAAGTGGACTCTGGGTCAGGTCACCTGTGACCTGTTTATCGCCCTGGATGT
GCTGTGCTGCACTTCGTCCATCCTGCACCTGTGCGCCATCGCGCTAGACAGGTACTGGGCAATCACCGAC
CCTATAGACTACGTGAACAAGAGGACGCCCCGGCGCGCCGCTGCGCTGATCTCGCTCACTTGGCTCATTG
GCTTTCTCATCTCCATCCCGCCTATGCTGGGCTGGCGCACCCCGGAAGACCGCTCGAACCCCAACGAGTG
CACCATCAGCAAGGACCACGGGTACACCATCTACTCCACTTTCGGCGCTTTCTATATTCCGCTGCTGCTC
ATGCTGGTCCTCTATGGGCGCATCTTCAGAGCCGCGCGCTTCCGAATCCGCAAGACGGTCAAGAAGGTGG
AAAAGAAGGGAGCGGGCACCAGCTTCGGAACATCGTCGGCCCCGCCCCCCAAGAAGAGCCTGAATGGTCA
GCCAGGTAGTGGGGACTGCAGGCGCAGTGCTGAGAACAGGGCGGTGGGGACTCCGTGCGCTAATGGGGCG
GTGAGACAGGGTGAGGACGACGCCACCCTGGAGGTGATCGAGGTGCATCGAGTGGGCAACTCCAAAGGGC
ACCTTCCTCTGCCCAGCGAATCAGGAGCTACCTCCTATGTCCCCGCCTGCTTGGAGAGAAAAAATGAGCG
CACTGCTGAGGCAAAGCGCAAGATGGCCTTGGCCCGTGAGAGGAAGACAGTGAAGACACTGGGCATCATC
ATGGGCACCTTCATCCTCTGCTGGCTGCCCTTTTTCATTGTGGCCCTGGTCCTACCTTTCTGTGAGAGCA
GTTGCCACATGCCTGAGTTGTTGGGTGCCATAATTAACTGGCTAGGCTACTCCAACTCCCTGCTCAACCC
AGTTATTTATGCTTATTTCAACAAAGACTTTCAAAACGCTTTTAAGAAGATCATCAAGTGCAAGTTCTGC
CGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR227083 representing NM_008308
Red=Cloning site Green=Tags(s)

MDMFSLGQGNNTTTSLEPFGTGGNDTGLSNVTFSYQVITSLLLGTLIFCAVLGNACVVAAIALERSLQNV
ANYLIGSLAVTDLMVSVLVLPMAALYQVLNKWTLGQVTCDLFIALDVLCCTSSILHLCAIALDRYWAITD
PIDYVNKRTPRRAAALISLTWLIGFLISIPPMLGWRTPEDRSNPNECTISKDHGYTIYSTFGAFYIPLLL
MLVLYGRIFRAARFRIRKTVKKVEKKGAGTSFGTSSAPPPKKSLNGQPGSGDCRRSAENRAVGTPCANGA
VRQGEDDATLEVIEVHRVGNSKGHLPLPSESGATSYVPACLERKNERTAEAKRKMALARERKTVKTLGII
MGTFILCWLPFFIVALVLPFCESSCHMPELLGAIINWLGYSNSLLNPVIYAYFNKDFQNAFKKIIKCKFC
R

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008308
ORF Size 1263 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008308.4, NP_032334.2
RefSeq Size 4441 bp
RefSeq ORF 1266 bp
Locus ID 15550
UniProt ID Q64264
Cytogenetics 13 56.92 cM
MW 46.6 kDa
Summary G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling inhibits adenylate cyclase activity and activates a phosphatidylinositol-calcium second messenger system that regulates the release of Ca(2+) ions from intracellular stores. Plays a role in the regulation of 5-hydroxytryptamine release and in the regulation of dopamine and 5-hydroxytryptamine metabolism. Plays a role in the regulation of dopamine and 5-hydroxytryptamine levels in the brain, and thereby affects neural activity, mood and behavior. Plays a role in the response to anxiogenic stimuli.UniProtKB/Swiss-Prot Function
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Documents
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"NM_008308" in other vectors (4)
SKU Description Size Price
MC208723 Htr1a (untagged) - Mouse 5-hydroxytryptamine (serotonin) receptor 1A (Htr1a), (10ug) 10 ug
$686.00
MG227083 Htr1a (tGFP-tagged) - Mouse 5-hydroxytryptamine (serotonin) receptor 1A (Htr1a), (10ug) 10 ug
$886.00
MR227083L3 Lenti ORF clone of Htr1a (Myc-DDK-tagged) - Mouse 5-hydroxytryptamine (serotonin) receptor 1A (Htr1a) 10 ug
$986.00
MR227083L4 Lenti ORF clone of Htr1a (mGFP-tagged) - Mouse 5-hydroxytryptamine (serotonin) receptor 1A (Htr1a) 10 ug
$986.00

Plasmid Map for MR227083

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR227083
Circular map for MR227083

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