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Rpl29 (NM_009082) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR227076
Rpl29 (Myc-DDK-tagged) - Mouse ribosomal protein L29 (Rpl29)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $289.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rpl29
Synonyms Rpl43
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR227076 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCAAGTCCAAGAACCACACCACACACAACCAGTCCCGCAAATGGCACAGAAATGGCATCAAGAAAC
CCCGGTCGCAAAGATACGAATCTCTTAAGGGGGTTGACCCCAAGTTCCTGAGGAACATGCGCTTTGCCAA
GAAGCACAACAAGAAAGGCCTGAAGAAGATGCAGGCCAACAATGCAAAGGCAGTGAGTGCGCGCGCAGAG
GCCATCAAGGCCCTGGTGAAGCCTCAGGCCATCAAGCCCAAGATGCCAAAAGGCCCCAAACTCAAGCGGC
TGGCTTTCATCGCTCACCCCAAGCTTGGGAAGCGGATTCGAAGCTACATGGCCAAGGGTCAGAGGCTCTG
CCAACCGAAGCCTAAGGTCCAAACCAAGGCAGGGGCCAAAGCTCCAGCTAAGGCCCAGGCTTCAGCTCCA
GCTCAGGCTCCCAAAGGTGCTCAGGCCCCCAAAGGTGCCCAGGCCCCTGTGAAGGCCCCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR227076 protein sequence
Red=Cloning site Green=Tags(s)

MAKSKNHTTHNQSRKWHRNGIKKPRSQRYESLKGVDPKFLRNMRFAKKHNKKGLKKMQANNAKAVSARAE
AIKALVKPQAIKPKMPKGPKLKRLAFIAHPKLGKRIRSYMAKGQRLCQPKPKVQTKAGAKAPAKAQASAP
AQAPKGAQAPKGAQAPVKAP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009082
ORF Size 480 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009082.3, NP_033108.1
RefSeq Size 710 bp
RefSeq ORF 483 bp
Locus ID 19944
UniProt ID P47915
Cytogenetics 9 F1
MW 17.6 kDa
Summary Component of the large ribosomal subunit.UniProtKB/Swiss-Prot Function
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Documents
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"NM_009082" in other vectors (4)
SKU Description Size Price
MC209325 Rpl29 (untagged) - Mouse ribosomal protein L29 (Rpl29), (10ug) 10 ug
$165.00
MG227076 Rpl29 (tGFP-tagged) - Mouse ribosomal protein L29 (Rpl29), (10ug) 10 ug
$350.00
MR227076L3 Lenti ORF clone of Rpl29 (Myc-DDK-tagged) - Mouse ribosomal protein L29 (Rpl29) 10 ug
$450.00
MR227076L4 Lenti ORF clone of Rpl29 (mGFP-tagged) - Mouse ribosomal protein L29 (Rpl29) 10 ug
$450.00

Plasmid Map for MR227076

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR227076
Circular map for MR227076

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