Ago2 (NM_153178) Mouse Tagged ORF Clone

SKU MR227026

Ago2 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2C, 2 (Eif2c2)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Ago2
• Synonyms: 1110029L17Rik; 2310051F07Rik; AI225898; AL022874; AW546247; Eif2c2; ENSMUSG00000072493; Gerp95
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227026 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTACTCGGGAGCCGGCCCCGTTCTTGCTTCTCCTGCTCCGACAACATCACCCATCCCAGGATATGCCT; TCAAACCTCCACCTCGGCCGGACTTCGGCACCACCGGGAGAACAATCAAACTACAGGCCAATTTCTTTGA; AATGGACATCCCCAAAATTGACATCTATCACTATGAATTGGACATCAAACCTGAGAAATGCCCTCGGAGA; GTGAACAGGGAAATTGTGGAGCACATGGTCCAGCACTTTAAAACTCAGATCTTCGGGGACCGGAAGCCAG; TGTTTGATGGAAGGAAGAATCTGTACACAGCAATGCCCCTTCCGATCGGCAGGGACAAGGTGGAGCTGGA; GGTCACGCTCCCGGGAGAAGGCAAAGATCGCATCTTTAAGGTATCCATCAAGTGGGTGTCGTGCGTGAGC; CTGCAGGCGTTACACGATGCACTTTCGGGGCGGCTGCCCAGCGTCCCCTTCGAGACGATCCAGGCCCTGG; ACGTTGTCATGAGGCACTTACCATCCATGAGGTACACCCCTGTTGGCCGTTCCTTCTTCACTGCATCTGA; AGGCTGTTCCAACCCTCTGGGTGGGGGCAGAGAAGTGTGGTTTGGCTTCCATCAGTCCGTCCGACCTTCT; CTTTGGAAAATGATGCTGAATATTGATGTATCGGCAACAGCGTTTTACAAGGCACAGCCAGTGATCGAGT; TTGTTTGTGAAGTTTTGGATTTTAAAAGTATTGAAGAACAACAAAAACCTCTGACAGATTCCCAAAGGGT; AAAGTTTACCAAAGAAATCAAAGGTCTAAAGGTGGAGATAACGCACTGTGGTCAGATGAAGAGGAAGTAC; CGTGTCTGCAATGTGACCCGGCGGCCTGCCAGTCACCAAACGTTCCCACTGCAGCAGGAGAGTGGGCAGA; CAGTGGAGTGTACAGTGGCCCAGTACTTCAAGGACAGGCACAAGCTGGTTCTGCGCTACCCCCACCTCCC; GTGTTTACAAGTCGGACAGGAGCAGAAACACACCTACCTTCCTTTGGAGGTCTGTAACATAGTTGCTGGA; CAGAGATGTATAAAAAAATTAACAGACAATCAGACCTCAACCATGATCAGAGCAACTGCCAGGTCAGCAC; CTGATCGCCAAGAGGAGATCAGCAAACTGATGCGAAGTGCAAGTTTCAATACAGACCCATATGTTCGTGA; ATTTGGAATCATGGTGAAAGATGAGATGACAGATGTGACTGGGCGGGTTCTGCAGCCGCCCTCCATCCTC; TACGGGGGCAGGAACAAAGCAATTGCCACCCCTGTCCAGGGTGTGTGGGACATGCGAAACAAGCAGTTCC; ACACGGGCATCGAGATCAAGGTGTGGGCCATCGCCTGCTTCGCTCCCCAGCGCCAGTGTACAGAAGTCCA; TCTCAAGTCCTTCACAGAGCAACTGAGGAAGATCTCGAGAGATGCTGGGATGCCGATCCAGGGTCAGCCC; TGCTTCTGTAAATACGCACAGGGTGCAGACAGTGTGGAGCCCATGTTCCGACACCTGAAGAACACATACG; CTGGCCTCCAGCTGGTGGTGGTCATCCTGCCTGGCAAAACTCCTGTGTATGCGGAAGTCAAGCGTGTGGG; AGACACAGTGCTGGGGATGGCCACACAGTGCGTCCAGATGAAGAACGTGCAGAGGACCACGCCACAGACC; CTATCCAATCTCTGCTTAAAGATCAATGTCAAACTGGGAGGCGTCAACAACATCCTGCTGCCACAGGGCA; GGCCTCCAGTGTTCCAGCAACCTGTCATCTTCCTGGGAGCCGATGTCACCCACCCACCAGCTGGGGATGG; GAAGAAACCTTCTATTGCTGCCGTCGTGGGCAGCATGGACGCACACCCCAACCGCTACTGTGCCACCGTG; CGCGTGCAGCAGCACCGACAGGAGATCATCCAGGACCTGGCTGCCATGGTACGAGAGTTGCTCATTCAGT; TCTACAAGTCCACCCGCTTCAAGCCCACCCGCATCATCTTCTACCGCGATGGCGTCTCCGAGGGCCAGTT; CCAGCAGGTTCTCCACCATGAGCTCCTGGCCATCAGAGAGGCCTGCATCAAGCTGGAGAAGGACTATCAG; CCAGGAATCACGTTCATCGTGGTGCAGAAGCGGCACCACACACGCCTCTTCTGCACAGACAAAAATGAGC; GGGTTGGGAAGAGTGGGAACATTCCCGCAGGCACAACCGTGGACACGAAGATCACCCACCCCACTGAGTT; TGACTTCTACCTGTGCAGTCACGCCGGCATCCAGGGGACAAGCCGTCCTTCCCACTACCACGTGCTTTGG; GATGACAATCGTTTTTCTTCTGATGAGCTGCAGATTCTGACCTACCAGCTGTGTCACACCTACGTGCGCT; GCACACGCTCTGTGTCAATACCCGCGCCAGCTTACTATGCTCACCTGGTGGCCTTCCGAGCCAGGTACCA; CCTGGTGGATAAAGAACATGACAGCGCTGAAGGAAGCCATACCTCTGGGCAGAGCAACGGGCGAGATCAC; CAAGCGCTGGCCAAGGCGGTCCAGGTCCACCAGGACACACTGCGCACCATGTACTTTGCT; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227026 protein sequence; Red=Cloning site Green=Tags(s); ; MYSGAGPVLASPAPTTSPIPGYAFKPPPRPDFGTTGRTIKLQANFFEMDIPKIDIYHYELDIKPEKCPRR; VNREIVEHMVQHFKTQIFGDRKPVFDGRKNLYTAMPLPIGRDKVELEVTLPGEGKDRIFKVSIKWVSCVS; LQALHDALSGRLPSVPFETIQALDVVMRHLPSMRYTPVGRSFFTASEGCSNPLGGGREVWFGFHQSVRPS; LWKMMLNIDVSATAFYKAQPVIEFVCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQMKRKY; RVCNVTRRPASHQTFPLQQESGQTVECTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAG; QRCIKKLTDNQTSTMIRATARSAPDRQEEISKLMRSASFNTDPYVREFGIMVKDEMTDVTGRVLQPPSIL; YGGRNKAIATPVQGVWDMRNKQFHTGIEIKVWAIACFAPQRQCTEVHLKSFTEQLRKISRDAGMPIQGQP; CFCKYAQGADSVEPMFRHLKNTYAGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQT; LSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATV; RVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQ; PGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLW; DDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTSGQSNGRDH; QALAKAVQVHQDTLRTMYFA; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153178
• ORF Size: 2580 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_153178.4, NP_694818.3
• RefSeq Size: 8031 bp
• RefSeq ORF: 2583 bp
• Locus ID: 239528
• UniProt ID: Q8CJG0
• Cytogenetics: 15 D3
• MW: 97.3 kDa
• Summary: Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3' UTR). Can also up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3' UTR of the TNF (TNF-alpha) mRNA and up-regulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions. Regulates lymphoid and erythroid development and function, and this is independent of endonuclease activity.[UniProtKB/Swiss-Prot Function]