OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Micalcl (NM_027587) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MR226949
Micalcl (Myc-DDK-tagged) - Mouse MICAL C-terminal like (Micalcl)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $623.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (5)

TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "Micalcl or NM_027587"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Micalcl
Synonyms 4921517J23Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR226949 representing NM_027587
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACCAAAGAGCACCATCGCCTCCAAAGGAGCCGCCTCCTCCTCCATCTTTGTCTTCTTCATCTTCCC
TACCATCTTCATTTTCTTCAGCCAGTGTACCTGGGCACACTGCAGACGATTCCTCCTCTCCCCAGGTGCC
AGCTTACAACCTCCACTCCCCACAGATCTCCAGAGGCGATGTCAGCCCCACTCCAATCTACCTCAGACGT
GCCAGGGCTCAAGGGATTGTGAAGGAAATTCCCCTCTACCTGCCGCATAGCCCCATGCTAGAGAGCACAG
AAGACTGCCTGGTCGAGCCCGGTAGAGAAAGCCTCAGAAGTCCTGAGGAGATCTCCTCTTCAGAGGGCTG
CCAGGAAGCCCGGGCTCTTGGAAACACCAGAAGCATACAGCACCCCATTTTAGGGAAGGACCAGTATTTG
CCCAATCAAAACCTAGCTCTTGGTGCTGCAGGGAACCCTGGAGACCCGAGGGAGGAATCCAGGATGGGAC
AACCAGGTGGACCAGAGCTGTCCAAAGAAAGGAAGTTGGGCTTGAAGAAATTAGTCCTCACTGAGGAGCA
GAAGAACAAGTTGCTAGACTGGAGTGACTGCACCCAGGAGCACAAGACTGGGGAGCAGCTCTCTCAGGAA
AGTGCTGAGAACATCAGAGGTGGTAGCCTGAAGCCAACCTGCTCTTCTACCCTCTCTCAGGCGGTGAAAG
AGAAGCTGCTATCCCAGAAAAAGGCCCTGGGGGGGATGCGTACCCCAGCTGTCAAAGCACCTCAGGAGCG
AGAGGTGCCCCCACCCAAGTCCCCCCTGAAGCTCATAGCAAATGCCATCCTTAGGTCTCTGCTCCACAAC
TCCGAAGCTGGAAAGAAAACCAGCCCTAAGCCAGAGTCCAAAACTTTGCCTAGGGGCCAGCCACATGCTC
GTTCGTTCAGCCTCCGGAAACTGGGTTCCAGTAAAGATGGGGACCAGCAGTCTCCAGGGAGACACATGGC
CAAGAAGGCTTCAGCATTCTTCTCTCTGGCTTCTCCAACATCTAAGGTTGCCCAAGCCTCAGACCTTAGC
CTTCCCAACTCCATCCTCCGCAGCCGGAGTTTACCGAGTCGTCCATCCAAGATGTTCTTTTCAACCACAC
CTCACAGCAAGGTTGAAGATGTCCCCACACTCCTGGAGAAAGTGAGTTTGCAGGATGCCACTCACAGTCC
AAAGACAGGAGCCTCACATATTTCTTCCCTTGGCCTCAAAGATAAATCCTTTGAGAGTTTTCTCCAGGAA
TGCAAACAAAGAAAGGACATTGGGGACTTCTTTAATAGCCCCAAGGAGGAGGGGCCACCAGGGAACAGAG
TCCCATCCTTGGAGAAGTTGGTACAGCCTGTTGGCAGCACCTCCATGGGACAGGTGGCACATCCTTCTTC
CACAGGGCAAGATGCACACCCTGTAGCTCCAGTGACAGAAGCCACCTCTTCTCCCACCTCGTCCTCTGCA
GAGGAAGAAGCCGACTCCCAGCTTTCCTTAAGGATAAAGGAGAAGATACTCAGAAGAAGGAGGAAGCTGG
AAAAGCAGTCAGCTAAGCAAGAAGAATTGAAAAGACTCCATAAGGCTCAGGCCATCCAGAGACAACTAGA
GGAGGTGGAGGAGCGGCAGAGGACATTGGCGATCCAGGGCGTGAAGCTGGAGAAGGTGCTGAGAGGAGAA
GCAGATTCAGGGACACAGGATGAAGCCCAGCTTTTGCAGGAATGGTTTAAGCTGGTTCTGGAGAAGAATA
AATTAATGCGATATGAGTCGGAGCTACTGATCATGGCCCAAGAACTAGAACTAGAAGATCATCAGAGCAG
ACTGGAGCAGAAACTACGACAGAAAATGCTCAAGGATGAGGGCCAGAAAGACGAGAATGACCTAAAGGAG
GAGCAAGAAATATTCGAGGAGATGATGCAGGTGATTGAGCAAAGAAACAAGCTCGTGGATTCCCTAGAGG
AACAGCGGGTCAAAGAAAGAACCCAAGACCAACACTTTGAAAACTTTGTTCTCTCCAGAGGCTGTCAGCT
CAGCAGGACT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR226949 representing NM_027587
Red=Cloning site Green=Tags(s)

MNQRAPSPPKEPPPPPSLSSSSSLPSSFSSASVPGHTADDSSSPQVPAYNLHSPQISRGDVSPTPIYLRR
ARAQGIVKEIPLYLPHSPMLESTEDCLVEPGRESLRSPEEISSSEGCQEARALGNTRSIQHPILGKDQYL
PNQNLALGAAGNPGDPREESRMGQPGGPELSKERKLGLKKLVLTEEQKNKLLDWSDCTQEHKTGEQLSQE
SAENIRGGSLKPTCSSTLSQAVKEKLLSQKKALGGMRTPAVKAPQEREVPPPKSPLKLIANAILRSLLHN
SEAGKKTSPKPESKTLPRGQPHARSFSLRKLGSSKDGDQQSPGRHMAKKASAFFSLASPTSKVAQASDLS
LPNSILRSRSLPSRPSKMFFSTTPHSKVEDVPTLLEKVSLQDATHSPKTGASHISSLGLKDKSFESFLQE
CKQRKDIGDFFNSPKEEGPPGNRVPSLEKLVQPVGSTSMGQVAHPSSTGQDAHPVAPVTEATSSPTSSSA
EEEADSQLSLRIKEKILRRRRKLEKQSAKQEELKRLHKAQAIQRQLEEVEERQRTLAIQGVKLEKVLRGE
ADSGTQDEAQLLQEWFKLVLEKNKLMRYESELLIMAQELELEDHQSRLEQKLRQKMLKDEGQKDENDLKE
EQEIFEEMMQVIEQRNKLVDSLEEQRVKERTQDQHFENFVLSRGCQLSRT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027587
ORF Size 2040 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027587.2, NP_081863.2
RefSeq Size 2397 bp
RefSeq ORF 2043 bp
Locus ID 100504195
UniProt ID Q9D5U9
Cytogenetics 7 F1
MW 75.3 kDa
Summary May cooperate with MAPK1/ERK2 via an intracellular signal transduction pathway in the morphogenetic development of round spermatids to spermatozoa. May act as Rab effector protein and play a role in vesicle trafficking.UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_027587" in other vectors (3)
SKU Description Size Price
MG226949 Micalcl (tGFP-tagged) - Mouse MICAL C-terminal like (Micalcl), (10ug) 10 ug
$823.00
MR226949L3 Lenti ORF clone of Micalcl (Myc-DDK-tagged) - Mouse MICAL C-terminal like (Micalcl) 10 ug
$923.00
MR226949L4 Lenti ORF clone of Micalcl (mGFP-tagged) - Mouse MICAL C-terminal like (Micalcl) 10 ug
$923.00

Plasmid Map for MR226949

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR226949
Circular map for MR226949

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products