Ctgf (NM_010217) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR226802
Ctgf (Myc-DDK-tagged) - Mouse connective tissue growth factor (Ctgf)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ctgf
Synonyms Ccn2; fisp-12; Fisp12; Hcs24
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR226802 representing NM_010217
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTCGCCTCCGTCGCAGGTCCCATCAGCCTCGCCTTGGTGCTCCTCGCCCTCTGCACCCGGCCTGCTA
CGGGCCAGGACTGCAGCGCGCAATGTCAGTGCGCAGCCGAAGCAGCGCCGCACTGCCCCGCCGGCGTGAG
CCTGGTGCTGGACGGCTGCGGCTGCTGCCGCGTCTGCGCCAAGCAGCTGGGAGAACTGTGTACGGAGCGT
GACCCCTGCGACCCACACAAGGGCCTCTTCTGCGATTTCGGCTCCCCCGCCAACCGCAAGATCGGAGTGT
GCACTGCCAAAGATGGTGCACCCTGTGTCTTCGGTGGGTCGGTGTACCGCAGCGGTGAGTCCTTCCAAAG
CAGCTGCAAATACCAATGCACTTGCCTGGATGGGGCCGTGGGCTGCGTGCCCCTGTGCAGCATGGACGTG
CGCCTGCCCAGCCCTGACTGCCCCTTCCCGAGAAGGGTCAAGCTGCCTGGGAAATGCTGCGAGGAGTGGG
TGTGTGACGAGCCCAAGGACCGCACAGCAGTTGGCCCTGCCCTAGCTGCCTACCGACTGGAAGACACATT
TGGCCCAGACCCAACTATGATGCGAGCCAACTGCCTGGTCCAGACCACAGAGTGGAGCGCCTGTTCTAAG
ACCTGTGGGATGGGCATCTCCACCCGAGTTACCAATGACAATACCTTCTGCAGACTTGAGAAGCAGAGTC
GCCTCTGCATGGTCAGGCCCTGCGAAGCTGACCTGGAGGAAAACATTAAGAAGGGCAAAAAGTGCATCCG
GACACCTAAAATCGCCAAGCCTGTCAAGTTTGAGCTTTCTGGCTGCACCAGTGTGAAGACATACAGGGCT
AAGTTCTGCGGGGTGTGCACAGACGGCCGCTGCTGCACACCGCACAGAACCACCACTCTGCCAGTGGAGT
TCAAATGCCCCGATGGCGAGATCATGAAAAAGAATATGATGTTCATCAAGACCTGTGCCTGCCATTACAA
CTGTCCTGGGGACAATGACATCTTTGAGTCCCTGTACTACAGGAAGATGTACGGAGACATGGCG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR226802 representing NM_010217
Red=Cloning site Green=Tags(s)

MLASVAGPISLALVLLALCTRPATGQDCSAQCQCAAEAAPHCPAGVSLVLDGCGCCRVCAKQLGELCTER
DPCDPHKGLFCDFGSPANRKIGVCTAKDGAPCVFGGSVYRSGESFQSSCKYQCTCLDGAVGCVPLCSMDV
RLPSPDCPFPRRVKLPGKCCEEWVCDEPKDRTAVGPALAAYRLEDTFGPDPTMMRANCLVQTTEWSACSK
TCGMGISTRVTNDNTFCRLEKQSRLCMVRPCEADLEENIKKGKKCIRTPKIAKPVKFELSGCTSVKTYRA
KFCGVCTDGRCCTPHRTTTLPVEFKCPDGEIMKKNMMFIKTCACHYNCPGDNDIFESLYYRKMYGDMA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_010217
ORF Size 1044 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010217.1, NM_010217.2, NP_034347.2
RefSeq Size 2418 bp
RefSeq ORF 1047 bp
Locus ID 14219
UniProt ID P29268
Cytogenetics 10 11.84 cM
MW 38.3 kDa
Summary Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes (By similarity). Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells (By similarity). Enhances fibroblast growth factor-induced DNA synthesis (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_010217" in other vectors (3)
SKU Description Size Price
MG226802 Ctgf (tGFP-tagged) - Mouse connective tissue growth factor (Ctgf), (10ug) 10 ug
$489.00 $657.00
MR226802L3 Lenti ORF clone of Ctgf (Myc-DDK-tagged) - Mouse connective tissue growth factor (Ctgf) 10 ug
$757.00
MR226802L4 Lenti ORF clone of Ctgf (mGFP-tagged) - Mouse connective tissue growth factor (Ctgf) 10 ug
$757.00

Plasmid Map for MR226802

Download sequence for

Cloning scheme for MR226802

Circular map for MR226802

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