Ret (NM_009050) Mouse Tagged ORF Clone

SKU MR226724

Ret (Myc-DDK-tagged) - Mouse ret proto-oncogene (Ret), transcript variant 2

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Ret
• Synonyms: c-Ret; PTC; RET9; RET51
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226724 representing NM_009050; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCGAAAGCGACGTCCGGCGCCGCAGGGCTGGGGCTGAAGCTGATTTTGCTCCTGCCGCTGCTAGGAG; AAGCCCCACTGGGCCTCTATTTCTCAAGGGATGCTTACTGGGAGAGGCTGTATGTAGACCAGCCAGCTGG; CACACCTCTGCTCTATGTCCATGCCCTACGGGATGCCCCTGGAGAAGTGCCGAGCTTCCGCCTGGGCCAG; CATCTCTATGGCGTCTACCGTACACGGCTGCATGAGAATGACTGGATCCGCATCAATGAGACTACTGGCC; TTCTCTACCTCAATCAGAGCCTGGACCACAGTTCCTGGGAACAGCTCAGCATCCGCAATGGTGGTTTCCC; CCTGCTCACCATCTTCCTCCAGGTCTTTCTGGGGTCCACAGCCCAGAGAGAGGGAGAATGCCATTGGCCA; GGCTGTACCCGTGTGTACTTCTCCTTCATCAACGACACCTTCCCAAATTGTAGCTCCTTCAAAGCCCAGG; ATCTCTGCATCCCAGAGACAGCCGTGTCCTTCCGAGTCAGGGAGAACAGGCCTCCTGGCACCTTCTACCA; CTTCCACATGTTACCCGTGCAGTTCCTTTGTCCCAACATCAGTGTGAAGTACAGTCTCTTAGGAGGGGAT; AGTCTGCCCTTCCGTTGTGACCCAGACTGCCTGGAGGTGAGCACTCGCTGGGCCCTGGATCGAGAGCTCC; GGGAGAAGTATGTGCTGGAGGCTTTGTGCATAGTGGCAGGCCCTGGTGCCAACAAAGAGACGGTGACTCT; GTCCTTCCCAGTGACAGTGTATGATGAGGACGACTCGGCGCCCACCTTCTCTGGAGGTGTGGGCACTGCC; AGCGCGGTGGTGGAGTTTAAGCGGAAGGAGGGCACTGTGGTGGCCACCCTGCAGGTGTTCGATGCAGATG; TGGTGCCAGCGTCTGGGGAGCTGGTGAGACGGTACACAAACACACTCCTCTCAGGGGACTCCTGGGCCCA; GCAGACCTTCCGGGTGGAGCATTCGCCCATCGAGACCTTGGTCCAGGTCAACAACAACTCCGTTCGGGCA; ACCATGCACAATTACAAGCTGATTCTCAACAGGAGCCTGTCTATCTCAGAGAGCCGAGTCCTGCAGCTCG; CGGTCCTGGTCAACGACTCAGACTTCCAGGGGCCTGGGGCAGGTGGTATCCTCGTCCTCCATTTCAACGT; GTCTGTACTGCCCGTCACCCTGAACCTACCCAGGGCCTACTCCTTCCCAGTGAATAAGAGGGCCCGCCGC; TATGCCCAGATCGGGAAAGTCTGTGTGGAAAACTGCCAGGAGTTCAGCGGTGTCTCCATCCAGTACAAGC; TGCAGCCTTCCAGCATCAACTGCACTGCCCTAGGTGTGGTCACCTCACCCGAGGACACCTCGGGGACCCT; ATTTGTAAATGACACAGAGGCCCTGCGGCGACCTGAGTGCACCAAGCTTCAGTACACGGTGGTAGCCACT; GACCGGCAGACCCGCAGACAGACCCAGGCTTCGCTAGTGGTCACTGTGGAGGGGACATCCATTACTGAAG; AAGTAGGCTGCCCCAAGTCCTGTGCAGTAAACAAGAGGCGCCCCGAGTGTGAGGAATGTGGTGGCCTGGG; TTCTCCAACTGGCAGGTGCGAGTGGCGCCAGGGAGATGGTAAAGGGATCACCAGGAACTTCTCCACCTGC; TCCCCCAGTACCAGGACCTGCCCCGACGGCCACTGTGATGCTGTGGAGAGCCGGGATGCCAACATTTGCC; CCCAGGACTGTCTCCGTGCCGACATTGTTGGAGGACACGAGCGAGGGGAGCGCCAGGGCATTAAAGCAGG; CTACGGCATCTGCAACTGTTTCCCTGATGAGAAGAAATGCTTCTGCGAGCCAGAGGACAGCCAGGGCCCA; CTGTGTGATGCGCTGTGCCGCACGATCATCACAGCTGCCCTCTTCTCCCTTATCATCTCCATCCTGCTGT; CCATCTTCTGTGTCTGCCACCACCACAAGCATGGGCACAAGCCGCCCATTGCATCAGCGGAAATGACCTT; CTGCCGGCCGGCCCAGGGCTTCCCAATCAGTTATTCCTCCTCAGGCACCCGCCGGCCCTCACTGGATTCC; ACGGAGAACCAGGTTCCTGTGGACTCTTTCAAGATCCCGGAGGATCCAAAGTGGGAATTTCCTCGGAAGA; ACTTAGTTCTTGGGAAAACTCTGGGAGAAGGCGAGTTTGGAAAAGTTGTCAAGGCCACAGCCTTCCGTCT; GAAAGGCCGGGCAGGATACACCACAGTGGCTGTGAAAATGCTGAAAGAAAACGCCTCCCAGAGTGAGTTA; CGAGACCTGCTGTCTGAGTTCAACCTTCTGAAACAAGTCAACCATCCACATGTCATCAAGTTGTATGGGG; CCTGCAGCCAGGATGGGCCACTTCTTCTCATTGTGGAGTATGCCAAGTATGGCTCCCTGCGGGGATTCCT; CCGTGACAGCCGCAAGATTGGGCCTGCCTATGTGAGCGGTGGAGGCAGCCGCAACTCCAGCTCGCTGGAC; CACCCAGATGAAAGGGTACTGACCATGGGTGACCTCATCTCCTTCGCCTGGCAGATCTCGAGGGGTATGC; AGTACTTGGCAGAAATGAAGCTTGTACATCGGGACTTAGCTGCCAGGAACATCTTGGTGGCTGAGGGACG; GAAGATGAAGATTTCCGACTTTGGGCTGTCCCGAGATGTTTATGAGGAAGATTCCTATGTGAAGAAAAGC; AAGGGCCGGATTCCCGTCAAGTGGATGGCAATTGAGTCCCTTTTCGATCACATCTATACTACTCAAAGTG; ATGTGTGGTCCTTTGGAGTGCTGCTCTGGGAGATTGTGACCCTGGGAGGCAACCCCTACCCTGGAATTCC; TCCTGAACGACTCTTCAACCTTCTGAAGACAGGCCACAGGATGGAGAGGCCAGACAACTGCAGCGAGGAA; ATGTACCGTCTGATGCTGCAGTGCTGGAAGCAGGAGCCAGACAAGAGGCCAGTGTTTGCTGACATCAGCA; AGGATCTGGAGAAGATGATGGTCAAGAGCAGAGACTACTTGGACCTGGCTGCATCCACACCTTCGGACTC; ACTGCTGTATGACGATGGGCTCTCAGAAGAGGAGACACCCCTGGTGGACTGTAACAATGCTCCCCTCCCG; CGCTCCCTCCCTTCCACATGGATTGAAAACAAACTCTATGGCATGTCAGACCCGAACTGGCCTGGAGAGA; GTCCTGTACCACTCACGAGAGCCGATGGCACTAGCACTGGGTTCCCAAGATATGCAAATGATAGTGTATA; TGCTAACTGGATGGTTTCACCCTCAGCGGCAAAATTAATGGACACATTTGATAGC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226724 representing NM_009050; Red=Cloning site Green=Tags(s); ; MAKATSGAAGLGLKLILLLPLLGEAPLGLYFSRDAYWERLYVDQPAGTPLLYVHALRDAPGEVPSFRLGQ; HLYGVYRTRLHENDWIRINETTGLLYLNQSLDHSSWEQLSIRNGGFPLLTIFLQVFLGSTAQREGECHWP; GCTRVYFSFINDTFPNCSSFKAQDLCIPETAVSFRVRENRPPGTFYHFHMLPVQFLCPNISVKYSLLGGD; SLPFRCDPDCLEVSTRWALDRELREKYVLEALCIVAGPGANKETVTLSFPVTVYDEDDSAPTFSGGVGTA; SAVVEFKRKEGTVVATLQVFDADVVPASGELVRRYTNTLLSGDSWAQQTFRVEHSPIETLVQVNNNSVRA; TMHNYKLILNRSLSISESRVLQLAVLVNDSDFQGPGAGGILVLHFNVSVLPVTLNLPRAYSFPVNKRARR; YAQIGKVCVENCQEFSGVSIQYKLQPSSINCTALGVVTSPEDTSGTLFVNDTEALRRPECTKLQYTVVAT; DRQTRRQTQASLVVTVEGTSITEEVGCPKSCAVNKRRPECEECGGLGSPTGRCEWRQGDGKGITRNFSTC; SPSTRTCPDGHCDAVESRDANICPQDCLRADIVGGHERGERQGIKAGYGICNCFPDEKKCFCEPEDSQGP; LCDALCRTIITAALFSLIISILLSIFCVCHHHKHGHKPPIASAEMTFCRPAQGFPISYSSSGTRRPSLDS; TENQVPVDSFKIPEDPKWEFPRKNLVLGKTLGEGEFGKVVKATAFRLKGRAGYTTVAVKMLKENASQSEL; RDLLSEFNLLKQVNHPHVIKLYGACSQDGPLLLIVEYAKYGSLRGFLRDSRKIGPAYVSGGGSRNSSSLD; HPDERVLTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNILVAEGRKMKISDFGLSRDVYEEDSYVKKS; KGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPERLFNLLKTGHRMERPDNCSEE; MYRLMLQCWKQEPDKRPVFADISKDLEKMMVKSRDYLDLAASTPSDSLLYDDGLSEEETPLVDCNNAPLP; RSLPSTWIENKLYGMSDPNWPGESPVPLTRADGTSTGFPRYANDSVYANWMVSPSAAKLMDTFDS; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009050
• ORF Size: 3345 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_009050.2, NP_033076.2
• RefSeq Size: 6064 bp
• RefSeq ORF: 3348 bp
• Locus ID: 19713
• UniProt ID: P35546
• Cytogenetics: 6 55.86 cM
• MW: 124.3 kDa
• Summary: Receptor tyrosine-protein kinase involved in numerous cellular mechanisms including cell proliferation, neuronal navigation, cell migration, and cell differentiation upon binding with glial cell derived neurotrophic factor family ligands. Phosphorylates PTK2/FAK1. Regulates both cell death/survival balance and positional information. Required for the molecular mechanisms orchestration during intestine organogenesis; involved in the development of enteric nervous system and renal organogenesis during embryonic life, and promotes the formation of Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue. Modulates cell adhesion via its cleavage by caspase in sympathetic neurons and mediates cell migration in an integrin (e.g. ITGB1 and ITGB3)-dependent manner. Involved in the development of the neural crest. Active in the absence of ligand, triggering apoptosis through a mechanism that requires receptor intracellular caspase cleavage. Acts as a dependence receptor; in the presence of the ligand GDNF in somatotrophs (within pituitary), promotes survival and down regulates growth hormone (GH) production, but triggers apoptosis in absence of GDNF. Regulates nociceptor survival and size. Triggers the differentiation of rapidly adapting (RA) mechanoreceptors. Mediator of several diseases such as neuroendocrine cancers; these diseases are characterized by aberrant integrins-regulated cell migration. Mediates, through interaction with GDF15-receptor GFRAL, GDF15-induced cell-signaling in the brainstem which induces inhibition of food-intake. Activates MAPK- and AKT-signaling pathways (PubMed:28846099).[UniProtKB/Swiss-Prot Function]