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Htra3 (NM_001042615) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR226672
Htra3 (Myc-DDK-tagged) - Mouse HtrA serine peptidase 3 (Htra3), transcript variant 2
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Htra3 or NM_001042615"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Htra3
Synonyms 2210021K23Rik; 9530081K03Rik; Prsp; Tasp
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR226672 representing NM_001042615
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGGCGCGCGCGCTGCTCCCCGCCACGCTGGCCATTCTGGCCACGCTGGCTGTGTTGGCTCTGGCCC
GGGAGCCCCCAGCGGCTCCGTGTCCTGCGCGCTGCGACGTGTCGCGCTGTCCGAGCCCTCGCTGCCCTGG
GGGCTATGTGCCTGACCTCTGCAACTGCTGCCTGGTGTGCGCTGCCAGCGAGGGCGAGCCCTGCGGCCGC
CCCCTGGACTCTCCGTGCGGGGACAGTCTGGAGTGCGTGCGCGGCGTGTGCCGCTGCCGTTGGACCCACA
CTGTGTGTGGCACAGACGGGCATACTTATGCCGACGTGTGCGCGCTGCAGGCCGCCAGCCGTCGTGCGTT
GCAGGTCTCCGGGACTCCAGTGCGCCAGCTGCAGAAGGGTGCCTGTCCCTCTGGTCTCCACCAGCTGACC
AGTCCGCGGTACAAGTTCAACTTCATCGCCGATGTGGTGGAGAAGATTGCGCCAGCTGTGGTCCACATAG
AGCTCTTTCTGAGACACCCCCTGTTTGGCCGGAATGTGCCGCTGTCCAGTGGCTCGGGCTTCATCATGTC
AGAAGCCGGTTTGATCGTCACCAACGCCCACGTGGTCTCCAGCTCCAGCACTGCCTCCGGCCGGCAGCAG
CTGAAGGTGCAGCTGCAGAATGGGGATGCCTATGAGGCCACCATCCAGGACATCGACAAGAAGTCGGACA
TTGCCACGATTGTAATCCACCCCAAGAAAAAGCTCCCTGTGTTGCTGCTGGGTCACTCAGCAGACCTGCG
GCCTGGCGAGTTCGTGGTGGCCATCGGCAGCCCCTTTGCCCTGCAGAACACCGTGACAACGGGCATTGTC
AGCACTGCCCAGCGGGATGGCAAGGAGCTGGGTCTCCGGGACTCAGACATGGACTATATCCAGACCGATG
CCATCATCAATTACGGGAACTCAGGAGGACCCCTGGTGAACCTGGATGGCGAGGTCATCGGCATCAACAC
GCTCAAGGTTGCAGCTGGCATCTCCTTTGCCATCCCCTCAGATCGCATCACACGCTTCCTCTCTGAGTTC
CAAAACAAGCATGTGAAAGCCCTCTCACCAGCACTGCAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR226672 representing NM_001042615
Red=Cloning site Green=Tags(s)

MQARALLPATLAILATLAVLALAREPPAAPCPARCDVSRCPSPRCPGGYVPDLCNCCLVCAASEGEPCGR
PLDSPCGDSLECVRGVCRCRWTHTVCGTDGHTYADVCALQAASRRALQVSGTPVRQLQKGACPSGLHQLT
SPRYKFNFIADVVEKIAPAVVHIELFLRHPLFGRNVPLSSGSGFIMSEAGLIVTNAHVVSSSSTASGRQQ
LKVQLQNGDAYEATIQDIDKKSDIATIVIHPKKKLPVLLLGHSADLRPGEFVVAIGSPFALQNTVTTGIV
STAQRDGKELGLRDSDMDYIQTDAIINYGNSGGPLVNLDGEVIGINTLKVAAGISFAIPSDRITRFLSEF
QNKHVKALSPALH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001042615
ORF Size 1089 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001042615.2, NP_001036080.1
RefSeq Size 2211 bp
RefSeq ORF 1092 bp
Locus ID 78558
UniProt ID Q9D236
Cytogenetics 5 B3
MW 39 kDa
Summary Serine protease that cleaves beta-casein/CSN2 as well as several extracellular matrix (ECM) proteoglycans such as decorin/DCN, biglycan/BGN and fibronectin/FN1. Inhibits signaling mediated by TGF-beta family proteins possibly indirectly by degradation of these ECM proteoglycans (PubMed:15206957). May act as a tumor suppressor. Negatively regulates, in vitro, trophoblast invasion during placental development and may be involved in the development of the placenta in vivo. May also have a role in ovarian development, granulosa cell differentiation and luteinization (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001042615" in other vectors (4)
SKU Description Size Price
MC211891 Htra3 (untagged) - Mouse HtrA serine peptidase 3 (Htra3), transcript variant 2, (10ug) 10 ug
$503.00
MG226672 Htra3 (tGFP-tagged) - Mouse HtrA serine peptidase 3 (Htra3) transcript variant 2, (10ug) 10 ug
$657.00
MR226672L3 Lenti ORF clone of Htra3 (Myc-DDK-tagged) - Mouse HtrA serine peptidase 3 (Htra3), transcript variant 2 10 ug
$757.00
MR226672L4 Lenti ORF clone of Htra3 (mGFP-tagged) - Mouse HtrA serine peptidase 3 (Htra3), transcript variant 2 10 ug
$757.00

Plasmid Map for MR226672

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR226672
Circular map for MR226672

file-alt Citations

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