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Nts (NM_024435) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR226576
Nts (Myc-DDK-tagged) - Mouse neurotensin (Nts)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$289.00 $450.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Nts or NM_024435"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Nts
Synonyms 5033428E16Rik; NMN-125; NN; NT; NT/N; NTS1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR226576 representing NM_024435
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGAGGAATGAATCTCCAGCTGGTGTGCCTGACTCTCCTGGCTTTCAGCTCCTGGAGTCTGTGCTCAG
ATTCAGAAGAAGATGTGAGAGCCCTGGAGGCAGATCTATTGACAAACATGCATACATCCAAGATCAGCAA
AGCAAGTCCTCCGTCTTGGAAAATGACCTTGCTAAATGTTTGCAGCCTCATAAATAACGTGAACAGCCCG
GCCGAGGAAGCAGGAGACATGCATGATGACGACCTTGTTGGCAAAAGGAAACTTCCCCTTGTTCTGGATG
GTTTTAGCTTGGAAGCAATGCTGACCATCTTCCAGCTCCAGAAAATCTGCCGCAGCAGGGCCTTTCAACA
CTGGGAGATAATCCAGGAAGATATCCTTGATAACGTCAATGATAAAAACGAGAAGGAAGAAGTGATAAAG
AGAAAAATCCCTTATATTCTGAAAAGGCAGCTGTATGAAAATAAACCCAGAAGGCCCTACATTCTCAAGA
GGGGTTCCTACTACTAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR226576 representing NM_024435
Red=Cloning site Green=Tags(s)

MRGMNLQLVCLTLLAFSSWSLCSDSEEDVRALEADLLTNMHTSKISKASPPSWKMTLLNVCSLINNVNSP
AEEAGDMHDDDLVGKRKLPLVLDGFSLEAMLTIFQLQKICRSRAFQHWEIIQEDILDNVNDKNEKEEVIK
RKIPYILKRQLYENKPRRPYILKRGSYYY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024435
ORF Size 507 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024435.2, NP_077755.1
RefSeq Size 1237 bp
RefSeq ORF 510 bp
Locus ID 67405
UniProt ID Q9D3P9
Cytogenetics 10 D1
MW 20.1 kDa
Summary Neurotensin may play an endocrine or paracrine role in the regulation of fat metabolism. It causes contraction of smooth muscle (By similarity).UniProtKB/Swiss-Prot Function
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Documents
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"NM_024435" in other vectors (4)
SKU Description Size Price
MC210549 Nts (untagged) - Mouse neurotensin (Nts), (10ug) 10 ug
$480.00
MG226576 Nts (tGFP-tagged) - Mouse neurotensin (Nts), (10ug) 10 ug
$650.00
MR226576L3 Lenti ORF clone of Nts (Myc-DDK-tagged) - Mouse neurotensin (Nts) 10 ug
$750.00
MR226576L4 Lenti ORF clone of Nts (mGFP-tagged) - Mouse neurotensin (Nts) 10 ug
$750.00

Plasmid Map for MR226576

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR226576
Circular map for MR226576

file-alt Citations

Sleep Regulation by Neurotensinergic Neurons in a Thalamo-Amygdala Circuit
Ma, Zhong, Liu et al
Neuron (2019) 103 (2), 323-334.e7 DOI: 10.1016/j.neuron.2019.05.015
AI Summary: OriGene products pCMV6-Nts-FLAG, pCMV6-Slc32a1-FLAG, and pCMV6-Slc17a6-FLAG were used in this study to enable the identification and manipulation of specific neuronal populations involved in sleep regulation within the thalamo-amygdala circuit.
Snippet: pCMV6-Nts-FLAG OriGene Technologies Cat# MR226576 pCMV6-Slc32a1-FLAG OriGene Technologies Cat# MR208431 pCMV6-Slc17a6-FLAG OriGene Technologies Cat# MR209115 pU6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA Addgene Cat# 60229;RRID: Addgene_60229
Showing 1-1 of 1 papers.
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