Dna2 (NM_177372) Mouse Tagged ORF Clone

SKU MR226448

Dna2 (Myc-DDK-tagged) - Mouse DNA replication helicase 2 homolog (yeast) (Dna2)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Dna2
• Synonyms: Dna2l; E130315B21Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226448 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGAGCCGCTGGACGAGCTGGACCTGCTGCTGCTGGAAGAGGACGGCGGAGCGGAGGCCGTGCCGCGGG; TGGAGCTACTTCGGAAGAAGGCGGACGCTTTGTTTCCAGAGACAGTTCTGAGCCGAGGAGTGGATAACCG; GTACCTGGTGCTAGCTGTTGAGACCTCGCAGAATGAAAGAGGAGCCGAGGAGAAGCGCTTGCACGTCACG; GCGTCCCAGGACCGAGAGCACGAAGTTCTGTGCATCCTGAGGAATGGCTGGAGCTCAGTACCAGTTGAGC; CAGGGGACATCGTTCACTTAGAGGGAGACTGCACATCTGAGCCGTGGATAATAGACGATGACTTTGGATA; TTTTATTCTGTACCCGGACATGATGATCTCCGGCACAAGCGTCGCTAGCAGCATTCGGTGTCTGAGAAGA; GCTGTCCTGAGCGAGACCTTCAGGGGCTCTGATCCAGCCACTCGCCAGATGCTGATCGGTACAATTCTCC; ACGAAGTCTTTCAGAAAGCGATAAGTGAGAGCTTTGCTCCAGAAAGGCTACAAGAGCTTGCTCTTCAGAC; CCTCCGAGAAGTGAGGCATTTGAAGGAAATGTATCGCTTGAATCTGAGCCAAGATGAAATACTCTGTGAA; GTTGAGGAGTATCTCCCTTCTTTCTCTAAGTGGGCGGAGGACTTCATGCGTAAGGGCCCTTCGTCTGAAT; TCCCTCAAATGCAGCTCTCTCTGCCAAGCGATGGCAGTAACAGAAGTTCACCTTGTAACATTGAAGTAGT; CAAGTCACTGGATATCGAAGAAAGCATCTGGTCCCCTAGGTTTGGACTGAAGGGCAAAATAGACGTCACA; GTCGGGGTCAAAATACATCGAGATTGTAAAATGAAATACAAGGTAATGCCACTGGAGCTGAAGACCGGCA; AAGAGTCCAACTCCATCGAGCACCGGAGTCAGGTGGTCCTGTACACGCTGCTAAGCCAGGAGAGGCGAGA; GGACCCCGAGGCTGGCTGGCTTCTCTACCTCAAGACCGGGCAGATGTACCCTGTGCCCGCCAACCATCTG; GACAAAAGAGAACTGCTGAAGCTCAGGAACTGGCTGGCCGCCTCGCTGCTGCACCGTGTGAGCCGAGCAG; CTCCGGGGGAGGAAGCTCGGCTCTCTGCCTTGCCACAGATAATCGAGGAAGAGAAAACTTGTAAATACTG; TTCGCAGATAGGCAACTGTGCTCTTTATAGCAGAGCAGTTGAAGAGCAGGGAGATGACGCATCCATCCCA; GAAGCCATGCTGTCCAAAATCCAGGAAGAAACACGGCACCTGCAGCTGGCGCACTTGAAGTATTTCAGCC; TGTGGTGTCTCATGTTAACGCTGGAGTCGCAATCTAAAGACAACCGGAAGACTCACCAGAGTATCTGGTT; AACGCCTGCCTCTGAACTGGAGGAGAGTGGCAACTGTGTTGGAAACCTGGTCAGGACGGAGCCTGTATCA; AGAGTTTGTGATGGACAGTATTTACATAATTTTCAGCGGAAAAATGGTCCCATGCCAGCCACCAATCTGA; TGGCAGGTGACAGGATTATTTTAAGTGGAGAAGAGAGAAAACTATTTGCTTTGTCCAAAGGCTACGTGAA; GAAGATGAACAAGGCAGCCGTTACCTGCTTGCTAGACAGGAACTTGTCGACACTCCCAGCAACAACGGTG; TTTCGATTAGACCGGGAAGAAAGACATGGCGACATAAGTACTCCACTAGGAAATCTGTCTAAATTGATGG; AAAGCACAGATCCCAGCAAAAGACTTCGAGAATTAATCATCGATTTCAGGGAACCTCAGTTCATCGCCTA; CCTCAGCTCCGTCCTTCCACACGATGCGAAGGATACGGTGGCCAACATTCTAAAGGGTTTGAATAAACCC; CAGAGACAAGCAATGAAGAGAGTCCTTCTTTCGAAAGACTACACGCTCATTGTTGGGATGCCAGGGACAG; GAAAGACAACCACCATCTGTGCCCTGGTGAGGATCCTCTCTGCCTGTGGGTTCAGCGTTCTGCTGACCAG; CTACACGCACTCCGCCGTTGACAATATTCTCCTGAAGTTAGCCAAGTTTAAAGTCGGATTTTTGCGCTTG; GGTCAGTCTCATAAGGTCCATCCAGACATCCAGAAGTTTACAGAGGAGGAGATTTGCAGATCGAGGTCCA; TTGCGTCTTTAGCCCATCTAGAAGAACTCTATAACAGTCACCCTATTGTTGCAACAACCTGCATGGGAAT; AAATCACCCAATATTTTCCCGAAAAACCTTTGATTTCTGTATTGTGGATGAAGCATCTCAAATCAGCCAG; CCCGTCTGCCTGGGCCCACTGTTCTTTTCGCGGAGGTTTGTGCTGGTGGGGGACCATCAGCAGCTGCCTC; CCCTGGTTGTAAACCGAGAGGCAAGAGCTCTGGGCATGAGCGAGAGCTTGTTCAAGAGGCTGGAGCGGAA; CGAGAGCGCTGTGGTGCAGCTGACCGTGCAATACCGAATGAACAGGAAGATTATGTCCCTGAGCAACAAG; CTCACATACGCAGGGAAACTGGAGTGTGGGTCAGACCGAGTGGCCAACGCAGTGCTAGCCCTGCCCAACC; TCAAGGACGCAAGGCTGAGCCTCCAGCTTTATGCCGACTACTCTGACAGCCCCTGGCTGGCTGGAGTGTT; AGAGCCAGACAATCCCGTGTGTTTCCTTAACACAGACAAGGTCCCAGCTCCAGAGCAAGTTGAAAATGGT; GGTGTTAGCAATGTCACAGAAGCCAGACTCATTGTCTTCCTAACCTCAACTTTTATAAAGGCTGCAGCCC; CTCAGACATTGGCGTCATCGCCCCGTACCGACAGCAGCTGCGGATCATCAGCGACTTACTGGCCCGGTCT; TCTGTTGGGATGGTTGAGGTTAACACAG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226448 protein sequence; Red=Cloning site Green=Tags(s); ; MEPLDELDLLLLEEDGGAEAVPRVELLRKKADALFPETVLSRGVDNRYLVLAVETSQNERGAEEKRLHVT; ASQDREHEVLCILRNGWSSVPVEPGDIVHLEGDCTSEPWIIDDDFGYFILYPDMMISGTSVASSIRCLRR; AVLSETFRGSDPATRQMLIGTILHEVFQKAISESFAPERLQELALQTLREVRHLKEMYRLNLSQDEILCE; VEEYLPSFSKWAEDFMRKGPSSEFPQMQLSLPSDGSNRSSPCNIEVVKSLDIEESIWSPRFGLKGKIDVT; VGVKIHRDCKMKYKVMPLELKTGKESNSIEHRSQVVLYTLLSQERREDPEAGWLLYLKTGQMYPVPANHL; DKRELLKLRNWLAASLLHRVSRAAPGEEARLSALPQIIEEEKTCKYCSQIGNCALYSRAVEEQGDDASIP; EAMLSKIQEETRHLQLAHLKYFSLWCLMLTLESQSKDNRKTHQSIWLTPASELEESGNCVGNLVRTEPVS; RVCDGQYLHNFQRKNGPMPATNLMAGDRIILSGEERKLFALSKGYVKKMNKAAVTCLLDRNLSTLPATTV; FRLDREERHGDISTPLGNLSKLMESTDPSKRLRELIIDFREPQFIAYLSSVLPHDAKDTVANILKGLNKP; QRQAMKRVLLSKDYTLIVGMPGTGKTTTICALVRILSACGFSVLLTSYTHSAVDNILLKLAKFKVGFLRL; GQSHKVHPDIQKFTEEEICRSRSIASLAHLEELYNSHPIVATTCMGINHPIFSRKTFDFCIVDEASQISQ; PVCLGPLFFSRRFVLVGDHQQLPPLVVNREARALGMSESLFKRLERNESAVVQLTVQYRMNRKIMSLSNK; LTYAGKLECGSDRVANAVLALPNLKDARLSLQLYADYSDSPWLAGVLEPDNPVCFLNTDKVPAPEQVENG; GVSNVTEARLIVFLTSTFIKAAAPQTLASSPRTDSSCGSSATYWPGLLLGWLRLTQ; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177372
• ORF Size: 2898 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_177372.1, NM_177372.2, NM_177372.3, NP_796346.2
• RefSeq Size: 4122 bp
• RefSeq ORF: 3189 bp
• Locus ID: 327762
• UniProt ID: Q6ZQJ5
• Cytogenetics: 10 B4
• MW: 108.5 kDa
• Summary: Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break (DSB) repair: recruited by BLM and mediates the cleavage of 5'-ssDNA, while the 3'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA (ssDNA)-dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is subject to debate. According to various reports, the helicase activity is weak and its function remains largely unclear. Helicase activity may promote the motion of DNA2 on the flap, helping the nuclease function (By similarity).[UniProtKB/Swiss-Prot Function]