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Dna2 (NM_177372) Mouse Untagged Clone

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SKU
MC223434
Dna2 (untagged) - Mouse DNA replication helicase 2 homolog (yeast) (Dna2), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
  $1,018.00
6 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Dna2
Synonyms Dna2l; E130315B21Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC223434 representing NM_177372
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCCGCTGGACGAGCTGGACCTGCTGCTGCTGGAAGAGGACGGCGGAGCGGAGGCCGTGCCGCGGG
TGGAGCTACTTCGGAAGAAGGCGGACGCTTTGTTTCCAGAGACAGTTCTGAGCCGAGGAGTGGATAACCG
GTACCTGGTGCTAGCTGTTGAGACCTCGCAGAATGAAAGAGGAGCCGAGGAGAAGCGCTTGCACGTCACG
GCGTCCCAGGACCGAGAGCACGAAGTTCTGTGCATCCTGAGGAATGGCTGGAGCTCAGTACCAGTTGAGC
CAGGGGACATCGTTCACTTAGAGGGAGACTGCACATCTGAGCCGTGGATAATAGACGATGACTTTGGATA
TTTTATTCTGTACCCGGACATGATGATCTCCGGCACAAGCGTCGCTAGCAGCATTCGGTGTCTGAGAAGA
GCTGTCCTGAGCGAGACCTTCAGGGGCTCTGATCCAGCCACTCGCCAGATGCTGATCGGTACAATTCTCC
ACGAAGTCTTTCAGAAAGCGATAAGTGAGAGCTTTGCTCCAGAAAGGCTACAAGAGCTTGCTCTTCAGAC
CCTCCGAGAAGTGAGGCATTTGAAGGAAATGTATCGCTTGAATCTGAGCCAAGATGAAATACTCTGTGAA
GTTGAGGAGTATCTCCCTTCTTTCTCTAAGTGGGCGGAGGACTTCATGCGTAAGGGCCCTTCGTCTGAAT
TCCCTCAAATGCAGCTCTCTCTGCCAAGCGATGGCAGTAACAGAAGTTCACCTTGTAACATTGAAGTAGT
CAAGTCACTGGATATCGAAGAAAGCATCTGGTCCCCTAGGTTTGGACTGAAGGGCAAAATAGACGTCACA
GTCGGGGTCAAAATACATCGAGATTGTAAAATGAAATACAAGGTAATGCCACTGGAGCTGAAGACCGGCA
AAGAGTCCAACTCCATCGAGCACCGGAGTCAGGTGGTCCTGTACACGCTGCTAAGCCAGGAGAGGCGAGA
GGACCCCGAGGCTGGCTGGCTTCTCTACCTCAAGACCGGGCAGATGTACCCTGTGCCCGCCAACCATCTG
GACAAAAGAGAACTGCTGAAGCTCAGGAACTGGCTGGCCGCCTCGCTGCTGCACCGTGTGAGCCGAGCAG
CTCCGGGGGAGGAAGCTCGGCTCTCTGCCTTGCCACAGATAATCGAGGAAGAGAAAACTTGTAAATACTG
TTCGCAGATAGGCAACTGTGCTCTTTATAGCAGAGCAGTTGAAGAGCAGGGAGATGACGCATCCATCCCA
GAAGCCATGCTGTCCAAAATCCAGGAAGAAACACGGCACCTGCAGCTGGCGCACTTGAAGTATTTCAGCC
TGTGGTGTCTCATGTTAACGCTGGAGTCGCAATCTAAAGACAACCGGAAGACTCACCAGAGTATCTGGTT
AACGCCTGCCTCTGAACTGGAGGAGAGTGGCAACTGTGTTGGAAACCTGGTCAGGACGGAGCCTGTATCA
AGAGTTTGTGATGGACAGTATTTACATAATTTTCAGCGGAAAAATGGTCCCATGCCAGCCACCAATCTGA
TGGCAGGTGACAGGATTATTTTAAGTGGAGAAGAGAGAAAACTATTTGCTTTGTCCAAAGGCTACGTGAA
GAAGATGAACAAGGCAGCCGTTACCTGCTTGCTAGACAGGAACTTGTCGACACTCCCAGCAACAACGGTG
TTTCGATTAGACCGGGAAGAAAGACATGGCGACATAAGTACTCCACTAGGAAATCTGTCTAAATTGATGG
AAAGCACAGATCCCAGCAAAAGACTTCGAGAATTAATCATCGATTTCAGGGAACCTCAGTTCATCGCCTA
CCTCAGCTCCGTCCTTCCACACGATGCGAAGGATACGGTGGCCAACATTCTAAAGGGTTTGAATAAACCC
CAGAGACAAGCAATGAAGAGAGTCCTTCTTTCGAAAGACTACACGCTCATTGTTGGGATGCCAGGGACAG
GAAAGACAACCACCATCTGTGCCCTGGTGAGGATCCTCTCTGCCTGTGGGTTCAGCGTTCTGCTGACCAG
CTACACGCACTCCGCCGTTGACAATATTCTCCTGAAGTTAGCCAAGTTTAAAGTCGGATTTTTGCGCTTG
GGTCAGTCTCATAAGGTCCATCCAGACATCCAGAAGTTTACAGAGGAGGAGATTTGCAGATCGAGGTCCA
TTGCGTCTTTAGCCCATCTAGAAGAACTCTATAACAGTCACCCTATTGTTGCAACAACCTGCATGGGAAT
AAATCACCCAATATTTTCCCGAAAAACCTTTGATTTCTGTATTGTGGATGAAGCATCTCAAATCAGCCAG
CCCGTCTGCCTGGGCCCACTGTTCTTTTCGCGGAGGTTTGTGCTGGTGGGGGACCATCAGCAGCTGCCTC
CCCTGGTTGTAAACCGAGAGGCAAGAGCTCTGGGCATGAGCGAGAGCTTGTTCAAGAGGCTGGAGCGGAA
CGAGAGCGCTGTGGTGCAGCTGACCGTGCAATACCGAATGAACAGGAAGATTATGTCCCTGAGCAACAAG
CTCACATACGCAGGGAAACTGGAGTGTGGGTCAGACCGAGTGGCCAACGCAGTGCTAGCCCTGCCCAACC
TCAAGGACGCAAGGCTGAGCCTCCAGCTTTATGCCGACTACTCTGACAGCCCCTGGCTGGCTGGAGTGTT
AGAGCCAGACAATCCCGTGTGTTTCCTTAACACAGACAAGGTCCCAGCTCCAGAGCAAGTTGAAAATGGT
GGTGTTAGCAATGTCACAGAAGCCAGACTCATTGTCTTCCTAACCTCAACTTTTATAAAGGCAGGCTGCA
GCCCCTCAGACATTGGCGTCATCGCCCCGTACCGACAGCAGCTGCGGATCATCAGCGACTTACTGGCCCG
GTCTTCTGTTGGGATGGTTGAGGTTAACACAGTAGACAAATACCAAGGAAGGGACAAGAGTCTCATCCTG
GTGTCCTTTGTTAGGAGTAATGAGGATGGAACTCTCGGCGAGCTCCTGAAAGATTGGCGGAGACTCAACG
TGGCACTCACCAGAGCCAAGCATAAACTGATCCTGCTGGGCAGCGTGTCCTCGCTGAAGCGCTTCCCGCC
TCTGGGGACGCTCTTTGACCATCTGAATGCAGAGCAGTTAATCCTGGACCTTCCTTCCAGAGAGCATGAG
AGCCTCAGCCACATCCTGGGCGATTGTCAGCGAGACTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_177372
Insert Size 3189 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177372.3, NP_796346.2
RefSeq Size 4122 bp
RefSeq ORF 3189 bp
Locus ID 327762
UniProt ID Q6ZQJ5
Cytogenetics 10 B4
Summary Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break (DSB) repair: recruited by BLM and mediates the cleavage of 5'-ssDNA, while the 3'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA (ssDNA)-dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is subject to debate. According to various reports, the helicase activity is weak and its function remains largely unclear. Helicase activity may promote the motion of DNA2 on the flap, helping the nuclease function (By similarity).UniProtKB/Swiss-Prot Function
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"NM_177372" in other vectors (4)
SKU Description Size Price
MG226448 Dna2 (tGFP-tagged) - Mouse DNA replication helicase 2 homolog (yeast) (Dna2), (10ug) 10 ug
$789.00 $1,085.00
MR226448 Dna2 (Myc-DDK-tagged) - Mouse DNA replication helicase 2 homolog (yeast) (Dna2) 10 ug
$589.00 $885.00
MR226448L3 Lenti ORF clone of Dna2 (Myc-DDK-tagged) - Mouse DNA replication helicase 2 homolog (yeast) (Dna2) 10 ug
$1,185.00
MR226448L4 Lenti ORF clone of Dna2 (mGFP-tagged) - Mouse DNA replication helicase 2 homolog (yeast) (Dna2) 10 ug
$1,185.00

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