C3 (NM_009778) Mouse Tagged ORF Clone
$589.00
$1,422.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | C3 |
| Synonyms | AI255234; ASP; HSE-MSF; Plp |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_009778 |
| ORF Size | 4989 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_009778.3 |
| RefSeq Size | 5147 bp |
| RefSeq ORF | 4992 bp |
| Locus ID | 12266 |
| UniProt ID | P01027 |
| Cytogenetics | 17 29.72 cM |
| MW | 186.9 kDa |
| Summary | This gene encodes complement protein C3 which plays a central role in the classical, alternative and lectin activation pathways of the complement system. The encoded preproprotein undergoes a multi-step processing to generate various functional peptides. Mice deficient in the encoded protein fail to clear bacteria from the blood stream upon infection, display diminished airway hyperresponsiveness and lung eosinophilia upon allergen-induced pulmonary allergy, and develop severe lung injury after deposition of IgG immune complexes. Deficiency of the homolog of the encoded protein in humans was found to be associated with increased susceptibility to infections, age-related macular degeneration, and atypical hemolytic uremic syndrome. provided by RefSeq, Mar 2015 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_009778" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC224797 | C3 (untagged) - Mouse complement component 3 (C3), (10ug) | 10 ug |
$1,423.00
|
|
| MG226388 | C3 (tGFP-tagged) - Mouse complement component 3 (C3), (10ug) | 10 ug |
$1,622.00
|
|
| MR226388L3 | Lenti ORF clone of C3 (Myc-DDK-tagged) - Mouse complement component 3 (C3) | 10 ug |
$1,722.00
|
|
| MR226388L4 | Lenti ORF clone of C3 (mGFP-tagged) - Mouse complement component 3 (C3) | 10 ug |
$1,722.00
|
|
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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