Rab11fip2 (NM_001033172) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR226056
Rab11fip2 (Myc-DDK-tagged) - Mouse RAB11 family interacting protein 2 (class I) (Rab11fip2), transcript variant 1
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rab11fip2
Synonyms 4930470G04Rik; A830046J09Rik; AW558126; Nrip11
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR226056 representing NM_001033172
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGCTGTCTGAGCAAGCCCAAAAGTGGTTTCCAACCCACGTGCAGGTCACAGTGCTCCAAGCTAAAG
ATCTGAAGCCAAAAGGCAAAAGTGGTACCAATGACACATACACCATAATTCAGTTGGGCAAAGAGAAGTA
TTCTACATCTGTAGCAGAGAAAACCCTGGAGCCGGTCTGGAAGGAGGAGGCCTCCTTTGAGCTGCCTGGG
TTGCTCATGCAGGGTAGTCCGGAGAAATACATTCTTTTTCTTATAGTGATGCACAGGTCCCTGGTGGGAC
TGGATAAGTTTTTAGGGCAGGTGGCAATCAATCTCAATGACATCTTTGAGGACAAACAAAGAAGGAAAAC
AGAGTGGTTTAGATTAGAATCCAAACAAGGAAAAAGAATAAAAAACAGGGGTGAGATAAAGGTCAATATT
CAATTTATGAGGAACAACATGACAGCAAGCATGTTTGACTTGTCAATGAAGGACAAGACGAGATCACCTT
TTGCAAAATTAAAAGATAAGATGAAAGGGAGGAAAAGCGACGGGGTGTTTTCTGATACGTCCTCTGCCAT
CGTTCCGAGCACTCACATGCCTGATGCCAATCCTGAGTTTTCAAGTGGTGAAATGCAGATGAAATCCAAA
CCAAAAAAGCCTTTTCTTTTGGGTCCTCAGCGGCTCTCTTCTGCCCATTCGATGTCTGATTTAACTGGGT
CCCACTTATCTTCTGAGAAGCTGAAGTCCAGCACTGTGGGTCCAACACATCTTCTCAGTCGCCAGATAGA
TTCCTTTGGAGTTGTTCCAGAAAGTGGAAGTCTCAAGTCTCCACACAGACGAACACTAAGCTTTGATACT
TCTAAATTGAACCAACCTGGCAGCATTGTGGATGAAGGTGAACACTCTTTTGGAAGACAGAGTGACCCAT
TTACAAATGTGACTGCTTCATTACCCCAAAAATTTGCAACACTGCCAAGGAAGAAGAATCCATTTGAAGA
AAGCAGTGAGCCATGGGACAGCAGCATGAATTTATTCTCAAAACCAATTGAAGTCAGGAAAGAAAGTAAG
AGAGAGAAAAGGGAGAAGGTGAGCCTCTTTGAAAGAGTGACTGGCAAGAGAGATAGCAGGAGACCTGACA
AGCTGAACAATGGTGGATCTGATAGCCCATGTGACTTGAAATCACCTAGTGCTTTTAGTGAAAACCGGCA
GGACTATTTTGAATATGAGTCAACTAACCCGTTTACAGCAAAATTCAGGGCTTCAACTATAATGCCATCT
TCAAGTTTTCATGTGAATCCAACAAGCAGTGAAGACCTCAGGAAAATCCCGGACAACAATCCTTTCGATG
CCACGGCTGGGTACCGGAGTCTGACCTACGAAGAGGTGCTGCAGGAGCTGGTGAAGCACAAAGAACTCCT
TCGGAGGAAGGACACCCATATCCGGGAGCTAGAGGACTACATTGACAACCTCCTCGTCAGGGTGATGGAA
GAGACACCCAGCATCCTGCGAGTGCCCTACGAGCCATCCAGAAAAGCTGTATACACCCAGGACCACTTGT
CACCCGGGCCACCCCACCTCTGTGGAGAAGCTACTTCAACCCACTCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR226056 representing NM_001033172
Red=Cloning site Green=Tags(s)

MMLSEQAQKWFPTHVQVTVLQAKDLKPKGKSGTNDTYTIIQLGKEKYSTSVAEKTLEPVWKEEASFELPG
LLMQGSPEKYILFLIVMHRSLVGLDKFLGQVAINLNDIFEDKQRRKTEWFRLESKQGKRIKNRGEIKVNI
QFMRNNMTASMFDLSMKDKTRSPFAKLKDKMKGRKSDGVFSDTSSAIVPSTHMPDANPEFSSGEMQMKSK
PKKPFLLGPQRLSSAHSMSDLTGSHLSSEKLKSSTVGPTHLLSRQIDSFGVVPESGSLKSPHRRTLSFDT
SKLNQPGSIVDEGEHSFGRQSDPFTNVTASLPQKFATLPRKKNPFEESSEPWDSSMNLFSKPIEVRKESK
REKREKVSLFERVTGKRDSRRPDKLNNGGSDSPCDLKSPSAFSENRQDYFEYESTNPFTAKFRASTIMPS
SSFHVNPTSSEDLRKIPDNNPFDATAGYRSLTYEEVLQELVKHKELLRRKDTHIRELEDYIDNLLVRVME
ETPSILRVPYEPSRKAVYTQDHLSPGPPHLCGEATSTHS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001033172
ORF Size 1587 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001033172.3, NP_001028344.2
RefSeq Size 5304 bp
RefSeq ORF 1590 bp
Locus ID 74998
UniProt ID G3XA57
Cytogenetics 19 D3
MW 60 kDa
Summary A Rab11 effector binding preferentially phosphatidylinositol 3,4,5-trisphosphate (PtdInsP3) and phosphatidic acid (PA) and acting in the regulation of the transport of vesicles from the endosomal recycling compartment (ERC) to the plasma membrane. Involved in insulin granule exocytosis. Also involved in receptor-mediated endocytosis and membrane trafficking of recycling endosomes, probably originating from clathrin-coated vesicles. Required in a complex with MYO5B and RAB11 for the transport of NPC1L1 to the plasma membrane. Also acts as a regulator of cell polarity. Plays an essential role in phagocytosis through a mechanism involving TICAM2, RAC1 and CDC42 Rho GTPases for controlling actin-dynamics.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001033172" in other vectors (4)
SKU Description Size Price
MC217617 Rab11fip2 (untagged) - Mouse RAB11 family interacting protein 2 (class I) (Rab11fip2), transcript variant 1, (10ug) 10 ug
$543.00
MG226056 Rab11fip2 (tGFP-tagged) - Mouse RAB11 family interacting protein 2 (class I) (Rab11fip2) transcript variant 1, (10ug) 10 ug
$692.00
MR226056L3 Lenti ORF clone of Rab11fip2 (Myc-DDK-tagged) - Mouse RAB11 family interacting protein 2 (class I) (Rab11fip2), transcript variant 1 10 ug
$792.00
MR226056L4 Lenti ORF clone of Rab11fip2 (mGFP-tagged) - Mouse RAB11 family interacting protein 2 (class I) (Rab11fip2), transcript variant 1 10 ug
$792.00

Plasmid Map for MR226056

Download sequence for

Cloning scheme for MR226056

Circular map for MR226056

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