Nr1i2 (NM_010936) Mouse Tagged ORF Clone
- TrueORF®
SKU
MR226044
Nr1i2 (Myc-DDK-tagged) - Mouse nuclear receptor subfamily 1, group I, member 2 (Nr1i2), transcript variant 1
$686.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Nr1i2 |
| Synonyms | mPXR; PXR; PXR.1; PXR.2; PXR1; SXR |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_010936 |
| ORF Size | 1293 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_010936.3, NP_035066.1 |
| RefSeq Size | 2540 bp |
| RefSeq ORF | 1296 bp |
| Locus ID | 18171 |
| UniProt ID | O54915 |
| Cytogenetics | 16 B3 |
| MW | 50 kDa |
| Summary | Nuclear receptor that binds and is activated by a variety of endogenous and xenobiotic compounds. Transcription factor that activates the transcription of multiple genes involved in the metabolism and secretion of potentially harmful xenobiotics, endogenous compounds and drugs. Response to specific ligands is species-specific, due to differences in the ligand-binding domain. Binds to a response element in the promoters of the CYP3A4 and ABCB1/MDR1 genes (By similarity). Activated by naturally occurring steroids such as pregnenolone and progesterone, the cholesterol metabolite 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol, synthetic glucocorticoids and antiglucocorticoids and 16-alpha-carbonitrile (PCN).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_010936" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC209057 | Nr1i2 (untagged) - Mouse nuclear receptor subfamily 1, group I, member 2 (Nr1i2), transcript variant 1, (10ug) | 10 ug |
$686.00
|
|
| MG226044 | Nr1i2 (tGFP-tagged) - Mouse nuclear receptor subfamily 1 group I member 2 (Nr1i2) transcript variant 1, (10ug) | 10 ug |
$886.00
|
|
| MR226044L3 | Lenti ORF clone of Nr1i2 (Myc-DDK-tagged) - Mouse nuclear receptor subfamily 1, group I, member 2 (Nr1i2), transcript variant 1 | 10 ug |
$986.00
|
|
| MR226044L4 | Lenti ORF clone of Nr1i2 (mGFP-tagged) - Mouse nuclear receptor subfamily 1, group I, member 2 (Nr1i2), transcript variant 1 | 10 ug |
$986.00
|
|
Citations
Chemical manipulation of an activation/inhibition switch in the nuclear receptor PXR
Garcia-Maldonado, Huber, Chai et al
Nat Commun (2024) 15 (1), 4054 DOI: 10.1038/s41467-024-48472-1
Garcia-Maldonado, Huber, Chai et al
Nat Commun (2024) 15 (1), 4054 DOI: 10.1038/s41467-024-48472-1
Cited by 24
AI Summary: In this study, the pCMV6-mPXR plasmid from OriGene (cat. # MR226044) was used to express mouse PXR, enabling functional and mechanistic analyses of PXR activation and antagonism.
Snippet: Supplementary Data 1. Construction of pGL3-CYP3A4-luc containing firefly luciferase under the control of a PXR-responsive CYP3A4 promoter has also been described33,60. pCMV6-mPXR was purchased from OriGene Technologies, MD, USA, (cat. # MR226044). pRL-TK was purchased from Promega. Construction of the bacterial pET-3a PXR LBD and PXRL428V LBD expression plasmids was described below. Protein exp
Fasting-sensitive SUMO-switch on Prox1 controls hepatic cholesterol metabolism
Alfaro, Dittner, Becker et al
EMBO Rep (2023)
Alfaro, Dittner, Becker et al
EMBO Rep (2023)
Cited by 14
AI Summary: OriGene products—Myc-tagged HNF4α (MR227662), Myc-tagged LRH-1 (MR225371), and Myc-tagged PXR (MR226044)—were used to study the interactions of Prox1 with key hepatic transcription factors in HEK293A cells, helping to elucidate the molecular mechanisms underlying fasting-sensitive regulation of cholesterol metabolism.
Snippet: HEK293A cells (Thermo Fischer Scientific—R70507) were transfected with mouse HA-tagged wild-type Prox1 (wt) or K556R mutant together with mouse Myc-tagged HNF4α (Origene—MR227662), Myc-tagged LRH-1 (Origene—MR225371) or Myc-tagged PXR (Origene—MR226044) using Lipofectamine 3000 with Plus Reagent
CITCO Directly Binds to and Activates Human Pregnane X Receptor
Lin, Bwayi, Wu et al
Mol. Pharmacol. (2020) 97 (3), 180-190 DOI: 10.1124/mol.119.118513
Lin, Bwayi, Wu et al
Mol. Pharmacol. (2020) 97 (3), 180-190 DOI: 10.1124/mol.119.118513
Cited by 27
AI Summary: OriGene’s CMV6-mPXR Kana-R plasmid was used in transient transfection experiments to express mouse PXR in HEK293 cells, enabling the study to compare CITCO’s effects on human and mouse PXR activation.
Snippet: Transient transfections of HEK293 cells were performed using Lipofectamine 3000 Reagent. Briefly, transfection mixture containing 625 µl of Opti-MEM Reduced Serum Medium with 5.0 µg of CYP3A4-luciferase plasmid DNA (Lin et al., 2008;Wang et al., 2013) and 0.25 µg of pRL-TK plasmid DNA (encoding Renilla luciferase as a control reporter) (Promega), with or without 0.25 µg of FLAG-hPXR wild type (WT)...
Activating the pregnane X receptor by imperatorin attenuates dextran sulphate sodium-induced colitis in mice
Liu, Zhang, Zheng et al
Br. J. Pharmacol. (2018) 175 (17), 3563-3580 DOI: 10.1111/bph.14424
Liu, Zhang, Zheng et al
Br. J. Pharmacol. (2018) 175 (17), 3563-3580 DOI: 10.1111/bph.14424
Probing ligand structure-activity relationships in pregnane X receptor (PXR): efavirenz and 8-hydroxyefavirenz exhibit divergence in activation
Narayanan, Lade, Heck et al
ChemMedChem (2018)
Narayanan, Lade, Heck et al
ChemMedChem (2018)
AI Summary: OriGene’s pCMV6-entry and pCMV6-XL4 expression vectors were used for transfection experiments to assess PXR activation by efavirenz and its analogs in the study.
Snippet: OriGene Technologies, Rockville, MD) or an empty vector (6 μg pCMV6 - entry or 2 μg pCMV6 - XL4 per flask;OriGene Technologies)
SPA70 is a potent antagonist of human pregnane X receptor
Lin, Wang, Chai et al
Nat Commun (2017) 8 (1), 741 DOI: 10.1038/s41467-017-00780-5
Lin, Wang, Chai et al
Nat Commun (2017) 8 (1), 741 DOI: 10.1038/s41467-017-00780-5
AI Summary: OriGene’s pCMV6-mPXR plasmid (Cat. No. MR226044) was used to express mouse pregnane X receptor in Hepa 1-6 cells for transactivation assays, enabling functional characterization of SPA70 as an hPXR antagonist in the study.
Snippet: For the mPXR transactivation assay, Hepa 1-6 cells were co-transfected with pCMV6-mPXR (Cat. No. MR226044, OriGene Technologies, Rockville, MD), pGL3-CYP3A4-luc and pRL-TK by using Lipofectamine 3000
Showing 1-6 of 8 papers.
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