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Scd1 (NM_009127) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR225893
Scd1 (Myc-DDK-tagged) - Mouse stearoyl-Coenzyme A desaturase 1 (Scd1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $686.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Scd1
Synonyms AA589638; ab; AI265570; Scd; Scd-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009127
ORF Size 1065 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009127.4, NP_033153.2
RefSeq Size 4844 bp
RefSeq ORF 1068 bp
Locus ID 20249
UniProt ID P13516
Cytogenetics 19 37.98 cM
MW 41.5 kDa
Summary Stearyl-CoA desaturase that utilizes O(2) and electrons from reduced cytochrome b5 to introduce the first double bond into saturated fatty acyl-CoA substrates. Catalyzes the insertion of a cis double bond at the Delta-9 position into fatty acyl-CoA substrates including palmitoyl-CoA and stearoyl-CoA (PubMed:11500518, PubMed:11533264, PubMed:16275639, PubMed:16443825, PubMed:26098370). Gives rise to a mixture of 16:1 and 18:1 unsaturated fatty acids (PubMed:11500518, PubMed:11533264, PubMed:16443825, PubMed:26098370). Plays an important role in lipid biosynthesis (PubMed:17127673, PubMed:10899171, PubMed:11500518, PubMed:11441127, PubMed:11533264, PubMed:12177411, PubMed:26098370). Plays an important role in regulating the expression of genes that are involved in lipogenesis and in regulating mitochondrial fatty acid oxidation (PubMed:12177411, PubMed:17127673, PubMed:24356954, PubMed:24295027). Plays an important role in body energy homeostasis (PubMed:17127673, PubMed:15210843, PubMed:24295027, PubMed:24356954). Contributes to the biosynthesis of membrane phospholipids, cholesterol esters and triglycerides (PubMed:10899171, PubMed:11500518, PubMed:11441127, PubMed:11533264, PubMed:12177411, PubMed:15210843, PubMed:26098370). Required for normal development of sebaceous glands (PubMed:17738154, PubMed:11533264). Required for the biosynthesis of normal levels of Delta-9 unsaturated fatty acids and 1-alkyl-2,3-diacylglycerol in the Harderian gland (PubMed:11500518). Required for normal production of meibum, an oily material that prevents drying of the cornea (PubMed:11533264).UniProtKB/Swiss-Prot Function
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"NM_009127" in other vectors (4)
SKU Description Size Price
MC209357 Scd1 (untagged) - Mouse stearoyl-Coenzyme A desaturase 1 (Scd1), (10ug) 10 ug
$686.00
MG225893 Scd1 (tGFP-tagged) - Mouse stearoyl-Coenzyme A desaturase 1 (Scd1), (10ug) 10 ug
$886.00
MR225893L3 Lenti ORF clone of Scd1 (Myc-DDK-tagged) - Mouse stearoyl-Coenzyme A desaturase 1 (Scd1) 10 ug
$986.00
MR225893L4 Lenti ORF clone of Scd1 (mGFP-tagged) - Mouse stearoyl-Coenzyme A desaturase 1 (Scd1) 10 ug
$986.00

Plasmid Map for MR225893

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Cloning scheme for MR225893
Circular map for MR225893

file-alt Citations

DNMT3a promotes osteoblast differentiation and alleviates osteoporosis via the PPARγ/SCD1/GLUT1 axis
Lu, Wang, Wu et al
Epigenomics (2022) 14 (12), 777-792 DOI: 10.2217/epi-2021-0391
Cited by 15
AI Summary: OriGene products, specifically the lentiviral particles pCMV6-Entry, pCMV6-PPARγ (MR208132), pCMV6-DNMT3a (MC200622), and pCMV6-SCD1 (MR225893), were used to manipulate gene expression in osteoblast cells, enabling the study of DNMT3a, PPARγ, and SCD1 roles in osteoblast differentiation and osteoporosis.
Snippet: 549V), and subsequently transduced with pCMV6-Entry, PCMV6-PPARγ (MR208132), PCMV6-DNMT3a (MC200622) or PCMV6-SCD1 (MR225893). All lentivirus particles were obtained from OriGene Technologies (MD, USA). After transduction, the cells were treated with 400 μg/ml G418 for 4 days [18] or with 5 μg/ml puromycin for 7 days [19]
An N-terminal di-proline motif is essential for fatty acid-dependent degradation of Δ9-desaturase in Drosophila
Murakami, Nagao, Juni et al
J. Biol. Chem. (2017)
AI Summary: OriGene contributed to this study by providing the cDNA encoding mouse SCD1, which was used to generate expression vectors for functional analysis of fatty acid-dependent degradation mechanisms.
Snippet: … cDNA encoding mouse SCD1 was purchased from ORIGENE. Expression vectors were established by inserting the coding sequences of DESAT1 and mouse SCD1 into EcoRI and ...;... … cDNA encoding mouse SCD1 was purchased from ORIGENE. Expression vectors were established by inserting the coding sequences of DESAT1 and mouse SCD1 into EcoRI and
Showing 1-2 of 2 papers.
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