Wt1 (NM_144783) Mouse Tagged ORF Clone
$766.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Wt1 |
| Synonyms | D630046I19Rik; Wt-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_144783 |
| ORF Size | 1551 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_144783.2, NP_659032.3 |
| RefSeq Size | 3092 bp |
| RefSeq ORF | 1554 bp |
| Locus ID | 22431 |
| Cytogenetics | 2 55.06 cM |
| MW | 56.5 kDa |
| Summary | This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It plays an essential role in the normal development of the urogenital system, and the orthologous human gene is mutated in a small subset of patients with Wilm's tumors. Alternative splicing has been noted for this gene, however, the full-length nature of these variants is not known. The mRNA for this gene has been shown to initiate translation from non-AUG (CUG) and AUG translation start sites, resulting in different isoforms. provided by RefSeq, Apr 2013 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_144783" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC217294 | Wt1 (untagged) - Mouse Wilms tumor 1 homolog (Wt1), (10ug) | 10 ug |
$485.00
|
|
| MG225851 | Wt1 (tGFP-tagged) - Mouse Wilms tumor 1 homolog (Wt1), (10ug) | 10 ug |
$966.00
|
|
| MR225851L3 | Lenti ORF clone of Wt1 (Myc-DDK-tagged) - Mouse Wilms tumor 1 homolog (Wt1) | 10 ug |
$1,066.00
|
|
| MR225851L4 | Lenti ORF clone of Wt1 (mGFP-tagged) - Mouse Wilms tumor 1 homolog (Wt1) | 10 ug |
$1,066.00
|
|
Citations
Hyaluronan synthase 2-mediated hyaluronan production mediates Notch1 activation and liver fibrosis
Yang, Noureddin, Liu et al
Sci Transl Med (2019) 11 (496) DOI: 10.1126/scitranslmed.aat9284
Yang, Noureddin, Liu et al
Sci Transl Med (2019) 11 (496) DOI: 10.1126/scitranslmed.aat9284
Cited by 168
AI Summary: In this study, OriGene’s CD44-expressing plasmid (catalog no. MR225851) was used to cotransfect HEK293 cells, enabling the authors to investigate the role of CD44 in HAS2 promoter activity during liver fibrosis research.
Snippet: …HEK293 cells were cotransfected with promoter constructs and CD44- or WT1-expressing plasmid (catalog no. MR225851, Origene) for 24 to 48 hours before the luciferase assay
Showing 1-1 of 1 papers.
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