Rorb (NM_001043354) Mouse Tagged ORF Clone
- TrueORF®
SKU
MR225771
Rorb (Myc-DDK-tagged) - Mouse RAR-related orphan receptor beta (Rorb), transcript variant 1
$289.00
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Rorb |
| Synonyms | hstp; Nr1f2; Rorbeta; RZR-beta; RZRB |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001043354 |
| ORF Size | 1377 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001043354.2, NP_001036819.1 |
| RefSeq Size | 3330 bp |
| RefSeq ORF | 1380 bp |
| Locus ID | 225998 |
| UniProt ID | Q8R1B8 |
| Cytogenetics | 19 B |
| MW | 52.5 kDa |
| Summary | The protein encoded by this gene is a member of the NR1 subfamily of nuclear hormone receptors. It is a DNA-binding protein that can bind as a monomer or as a homodimer to hormone response elements upstream of several genes to enhance the expression of those genes. The encoded protein has been shown to interact with NM23-2, a nucleoside diphosphate kinase involved in organogenesis and differentiation, and to help regulate the expression of some genes involved in circadian rhythm. Three transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Feb 2014 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001043354" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC216192 | Rorb (untagged) - Mouse RAR-related orphan receptor beta (Rorb), transcript variant 1, (10ug) | 10 ug |
$503.00
|
|
| MG225771 | Rorb (tGFP-tagged) - Mouse RAR-related orphan receptor beta (Rorb) transcript variant 1, (10ug) | 10 ug |
$657.00
|
|
| MR225771L3 | Lenti ORF clone of Rorb (Myc-DDK-tagged) - Mouse RAR-related orphan receptor beta (Rorb), transcript variant 1 | 10 ug |
$757.00
|
|
| MR225771L4 | Lenti ORF clone of Rorb (mGFP-tagged) - Mouse RAR-related orphan receptor beta (Rorb), transcript variant 1 | 10 ug |
$757.00
|
|
Citations
Extensive transcriptional and chromatin changes underlie astrocyte maturation in vivo and in culture
Lattke, Goldstone, Ellis et al
Nat Commun (2021) 12 (1), 4335 DOI: 10.1038/s41467-021-24624-5
Lattke, Goldstone, Ellis et al
Nat Commun (2021) 12 (1), 4335 DOI: 10.1038/s41467-021-24624-5
Cited by 119
AI Summary: In this study, OriGene products—including Rorb (MR226779L2), Dbx2 (MR209729L2), Lhx2 (MR207721L2), and Fezf2 (MR209729L2) expression plasmids—were used to forcibly express key transcription factors in vitro, enabling the authors to investigate their roles in driving the maturation-specific gene expression profiles of astrocytes.
Extensive transcriptional and chromatin changes underlie astrocyte maturation in vivo and in culture
Lattke, Goldstone, Guillemot
bioRxiv (2020)
Lattke, Goldstone, Guillemot
bioRxiv (2020)
Cited by 1
Snippet: Rorb, Dbx2, Lhx2, and Fezf2 open reading frame (ORF) constructs from Origene (MR225771, MR215327, MR206390, MR207270) were re-cloned into a modified version of the TetO-FUWEGFP lentiviral expression plasmid (Addgene #30130, (Vierbuchen et al., 2010)). … We then replaced Nr1d1 with Rorb, Dbx2, Lhx2 or Fezf2 to generate V5-tagged doxycycline-inducible lentiviral expression vectors for these transcription factors
Showing 1-2 of 2 papers.
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