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S100a4 (NM_011311) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR225750
S100a4 (Myc-DDK-tagged) - Mouse S100 calcium binding protein A4 (S100a4)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $289.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "S100a4 or NM_011311"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol S100a4
Synonyms 18A2; 42a; Capl; FSp1; metastasin; Mts1; PeL98; pk9a
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR225750 representing NM_011311
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAAGACCCTTGGAGGAGGCCCTGGATGTAATTGTGTCCACCTTCCACAAATACTCAGGCAAAGAGG
GTGACAAGTTCAAGCTGAACAAGACAGAGCTCAAGGAGCTACTGACCAGGGAGCTGCCTAGCTTCCTGGG
GAAAAGGACAGATGAAGCTGCATTCCAGAAGGTGATGAGCAACTTGGACAGCAACAGGGACAATGAAGTT
GACTTCCAGGAGTACTGTGTCTTCCTGTCCTGCATTGCCATGATGTGCAATGAATTCTTTGAGGGCTGCC
CAGATAAGGAGCCCCGGAAGAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR225750 representing NM_011311
Red=Cloning site Green=Tags(s)

MARPLEEALDVIVSTFHKYSGKEGDKFKLNKTELKELLTRELPSFLGKRTDEAAFQKVMSNLDSNRDNEV
DFQEYCVFLSCIAMMCNEFFEGCPDKEPRKK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_011311
ORF Size 303 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_011311.2, NP_035441.1
RefSeq Size 513 bp
RefSeq ORF 306 bp
Locus ID 20198
UniProt ID P07091
Cytogenetics 3 39.27 cM
MW 12.2 kDa
Summary Calcium-binding protein that plays a role in various cellular processes including motility, angiogenesis, cell differentiation, apoptosis, and autophagy (PubMed:20519440). Increases cell motility and invasiveness by interacting with non-muscle myosin heavy chain (NMMHC) IIA/MYH9 (PubMed:8051043). Mechanistically, promotes filament depolymerization and increases the amount of soluble myosin-IIA, resulting in the formation of stable protrusions facilitating chemotaxis (PubMed:8051043). Modulates also the pro-apoptotic function of TP53 by binding to its C-terminal transactivation domain within the nucleus and reducing its protein levels (By similarity). Within the extracellular space, stimulates cytokine production including granulocyte colony-stimulating factor and CCL24 from T-lymphocytes (PubMed:20103644). In addition, stimulates T-lymphocyte chemotaxis by acting as a chemoattractant complex with PGLYRP1 that promotes lymphocyte migration via CCR5 and CXCR3 receptors (By similarity).UniProtKB/Swiss-Prot Function
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"NM_011311" in other vectors (4)
SKU Description Size Price
MC209346 S100a4 (untagged) - Mouse S100 calcium binding protein A4 (S100a4), (10ug) 10 ug
$225.00
MG225750 S100a4 (tGFP-tagged) - Mouse S100 calcium binding protein A4 (S100a4), (10ug) 10 ug
$489.00
MR225750L3 Lenti ORF clone of S100a4 (Myc-DDK-tagged) - Mouse S100 calcium binding protein A4 (S100a4) 10 ug
$525.00
MR225750L4 Lenti ORF clone of S100a4 (mGFP-tagged) - Mouse S100 calcium binding protein A4 (S100a4) 10 ug
$525.00

Plasmid Map for MR225750

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR225750
Circular map for MR225750

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