Csf3 (NM_009971) Mouse Tagged ORF Clone
- TrueORF®
SKU
MR225697
Csf3 (Myc-DDK-tagged) - Mouse colony stimulating factor 3 (granulocyte) (Csf3)
$450.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Csf3 |
| Synonyms | Csfg; G-CSF; MGI-IG |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_009971 |
| ORF Size | 624 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_009971.1, NP_034101.1 |
| RefSeq Size | 1363 bp |
| RefSeq ORF | 627 bp |
| Locus ID | 12985 |
| UniProt ID | P09920, Q0VB73 |
| Cytogenetics | 11 62.45 cM |
| MW | 22.9 kDa |
| Summary | Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_009971" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC208344 | Csf3 (untagged) - Mouse colony stimulating factor 3 (granulocyte) (Csf3), (10ug) | 10 ug |
$450.00
|
|
| MG225697 | Csf3 (tGFP-tagged) - Mouse colony stimulating factor 3 (granulocyte) (Csf3), (10ug) | 10 ug |
$650.00
|
|
| MR225697L1 | Lenti ORF clone of Csf3 (Myc-DDK-tagged) - Mouse colony stimulating factor 3 (granulocyte) (Csf3) | 10 ug |
$750.00
|
|
| MR225697L2 | Lenti ORF clone of Csf3 (mGFP-tagged) - Mouse colony stimulating factor 3 (granulocyte) (Csf3) | 10 ug |
$750.00
|
|
| MR225697L3 | Lenti ORF clone of Csf3 (Myc-DDK-tagged) - Mouse colony stimulating factor 3 (granulocyte) (Csf3) | 10 ug |
$750.00
|
|
| MR225697L4 | Lenti ORF clone of Csf3 (mGFP-tagged) - Mouse colony stimulating factor 3 (granulocyte) (Csf3) | 10 ug |
$750.00
|
|
Citations
Aerobic Glycolysis Controls Myeloid-Derived Suppressor Cells and Tumor Immunity via a Specific CEBPB Isoform in Triple-Negative Breast Cancer
Li, Tanikawa, Kryczek et al
Cell Metab. (2018) 28 (1), 87-103.e6 DOI: 10.1016/j.cmet.2018.04.022
Li, Tanikawa, Kryczek et al
Cell Metab. (2018) 28 (1), 87-103.e6 DOI: 10.1016/j.cmet.2018.04.022
AI Summary: In this study, OriGene qPCR primers—including Mouse G-CSF (MP202847), Mouse GM-CSF (MP202843), Mouse LDHA (MP207257), Mouse ACTb (MP200232), Mouse IL-1b (MP206724), Mouse TGF-b (MP217184), Mouse CEBPB (MP201856), Mouse MMP9 (MP207904), Mouse LC3B (MP208170), Human LDHA (HP208683), Human GM-CSF (HP200702), and Human ACTB (HP204660)—were used to measure gene expression levels critical for investigating the molecular mechanisms linking glycolysis, myeloid-derived suppressor cells, and tumor immunity in triple-negative breast cancer.
Snippet: …However, the link between MDSCs and glycolysis.;Oligonucleotides Primers for colony This paper See Table S2 siRNA for mouse ULK1 Qiagen Cat# GS22241 siRNA for mouse FIP200 Qiagen Cat# GS12421 siRNA for mouse ATG5 Qiagen Cat# GS11793 siRNA for mouse LC3b Qiagen Cat# GS67443 Mouse G-CSF primer Origene Cat# MP202847 Mouse GM-CSF primer Origene Cat# MP202843 Mouse LDHA primer Origene Cat# MP207257 Mouse ACTb primer Origene Cat# MP200232 Mouse IL-1b primer Origene Cat# MP206724 Mouse TGF-b primer Origene Cat# MP217184 Mouse CEBPB primer Origene Cat# MP201856 Mouse MMP9 primer Origene Cat# MP207904 Mouse LC3B primer Origene Cat# MP208170 Human LDHA primer Origene Cat# HP208683 Human GM-CSF primer Origene Cat# HP200702 Human ACTB primer Origene Cat# HP204660
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
