Shc3 (NM_009167) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR225519
Shc3 (Myc-DDK-tagged) - Mouse src homology 2 domain-containing transforming protein C3 (Shc3)
  $686.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Shc3
Synonyms N-Shc; Rai; ShcC
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR225519 representing NM_009167
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTGCCACCAGGAAGAGCCGGGCCGGCGACGAGCCACTGCCCAGGCCCCCTCGGGGCGCGCCGCACA
CCAGCGATCAGGTGCTGGGGCCGGGAGTCACCTATGTGGTCAAGTACTTGGGCTGCATCGAAGTTCTGCG
CTCAATGAGGTCTCTTGACTTCAGTACAAGAACTCAGGTTACCAGGGAAGCCATCAGCCGTGTCTGCGAA
GCTGTGCCAGGTGCCAAAGGAGCCCTCAAGAAGAGAAAGCCGCCGAGTAAGATGCTGTCCAGCATCCTGG
GGAAGAGCAACCTCCAGTTCGCAGGGATGAGCATCTCCCTGACCATCTCCACCGCCAGCCTGAATCTGCG
CACTCCTGACTCCAAACAGATCATAGCGAACCATCATATGCGGTCTATCTCTTTTGCCTCAGGGGGAGAC
CCGGACACAACGGACTATGTTGCCTATGTCGCTAAGGACCCTGTCAATCGCAGAGCTTGCCACATTCTGG
AATGCTGTGACGGGCTAGCCCAAGATGTCATTGGCTCCATCGGACAAGCCTTTGAACTCCGGTTCAAACA
GTATTTGCAGTGCCCTTCCAAGGTTCCTGCCCTCCAGGACCGAATGCAGAGTCTGGATGAGCCATGGACT
GAAGAAGAGGGAGATGGCCCTGATCACCCATACTACAACAGCGTTCCCACCAAGATGCCTCCCCCAGGGG
GGTTTCTGGATGCTCGATTGAAAGGCAGACCCCACGCTCCTGAGGCAGCCCAGTTTGCAGGAAAAGAGCA
AACTTATTACCAGGGAAGACACTTAGGAGATACATTTGGTGAAGACTGGCAGCGAGCACCCACCAGGCAA
GGCTCCTTGGACATCTATAGCACAGCAGAAGGGAAAACTCACATGGTTCCTGTAGGAGAAACACCAACCT
ATGTCAACACCCAGCCAGTCCCACCACAGGTGTGGCCAGCAGCAACCAGCAGCACTGAGAGCAGCCCACG
GAAGGACCTCTTTGACATGAAGCCCTTTGAAGATGCCCTGAGAAACCAGCCCCTGGGCCCCATGTTGAGC
AAAGCCGCGTCTGTGGAGTGCATCAGCCCGGTCACACCCAGAGCCCCGGATGCCAGGATGCTGGAGGAGC
TTAACGCTGAGCCCTGGTACCAAGGAGAGATGAGCAGGAAGGAGGCAGAGGCCCTGCTACGGGAAGATGG
AGACTTCCTAGTGAGGAAGAGTACCACCAACCCCGGCTCCTTTGTCCTCACAGGCATGCACAATGGGCAG
GCCAAGCACCTGCTGCTGGTGGACCCGGAAGGCACGATCCGGACGAAGGACAGGGTCTTTGACAGCATCA
GCCACCTCATCAATTACCACCTCGAGAGCAGCCTGCCCATTGTCTCTGCCGGGAGTGAGCTTTGTCTCCA
GCAACCAGTGGAGAGGAAACCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR225519 representing NM_009167
Red=Cloning site Green=Tags(s)

MSATRKSRAGDEPLPRPPRGAPHTSDQVLGPGVTYVVKYLGCIEVLRSMRSLDFSTRTQVTREAISRVCE
AVPGAKGALKKRKPPSKMLSSILGKSNLQFAGMSISLTISTASLNLRTPDSKQIIANHHMRSISFASGGD
PDTTDYVAYVAKDPVNRRACHILECCDGLAQDVIGSIGQAFELRFKQYLQCPSKVPALQDRMQSLDEPWT
EEEGDGPDHPYYNSVPTKMPPPGGFLDARLKGRPHAPEAAQFAGKEQTYYQGRHLGDTFGEDWQRAPTRQ
GSLDIYSTAEGKTHMVPVGETPTYVNTQPVPPQVWPAATSSTESSPRKDLFDMKPFEDALRNQPLGPMLS
KAASVECISPVTPRAPDARMLEELNAEPWYQGEMSRKEAEALLREDGDFLVRKSTTNPGSFVLTGMHNGQ
AKHLLLVDPEGTIRTKDRVFDSISHLINYHLESSLPIVSAGSELCLQQPVERKP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009167
ORF Size 1422 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009167.4
RefSeq Size 1540 bp
RefSeq ORF 1425 bp
Locus ID 20418
UniProt ID Q61120
Cytogenetics 13 26.12 cM
MW 52.6 kDa
Summary Signaling adapter that couples activated growth factor receptors to signaling pathway in neurons. Involved in the signal transduction pathways of neurotrophin-activated Trk receptors in cortical neurons (By similarity).UniProtKB/Swiss-Prot Function
Reviews
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_009167" in other vectors (4)
SKU Description Size Price
MC216480 Shc3 (untagged) - Mouse src homology 2 domain-containing transforming protein C3 (Shc3), (10ug) 10 ug
$732.00
MG225519 Shc3 (tGFP-tagged) - Mouse src homology 2 domain-containing transforming protein C3 (Shc3), (10ug) 10 ug
$886.00
MR225519L3 Lenti ORF clone of Shc3 (Myc-DDK-tagged) - Mouse src homology 2 domain-containing transforming protein C3 (Shc3) 10 ug
$986.00
MR225519L4 Lenti ORF clone of Shc3 (mGFP-tagged) - Mouse src homology 2 domain-containing transforming protein C3 (Shc3) 10 ug
$986.00

Plasmid Map for MR225519

Download sequence for

Cloning scheme for MR225519

Circular map for MR225519

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products