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Tpcn2 (NM_146206) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR225477
Tpcn2 (Myc-DDK-tagged) - Mouse two pore segment channel 2 (Tpcn2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $670.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tpcn2
Synonyms D830047E22Rik; Gm35086
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR225477 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCAGAAGAGCAGCCCCTTCTGGGCCGGGGCCGAGGCAGTGGCCAGGTCCACTCCGGTGCTGCAG
CCGATCAGGAGCTCTGCATAGACCAGGCTGTGGTCTTCATTGAAGACGCCATAAAGTACCGCTCCATCTA
CCACCGCATGGATGCTGGTTCATTGTGGCTTTACCGCTGGTATTACTCGAACGTCTGCCAACGTGTGCTG
GGCTTCATCATTTTCCTGATCCTGATCTTGGCTTTTGTGGAGGTCCCATCTTCCTTCACAAAGACTGCAG
ATGTGCGCTACCGTTCCCAGCCCTGGCAGCCGCCCTGTGGCCTGACCGAGACGATCGAGGCGTTCTGCTT
GCTTGCCTTCCTGGTTGACCTCTCTGTGAAGGGCTACTTGGTTGGGCAGGCCCAGTTGCAGCAGAACCTG
TGGCTGCTGGCCTACTTTATGGTGCTGGTTGTGTCTGTGGTGGACTGGATCGTTTCGCTGAGCCTTGCTT
GTGAGGAGCCCCTGCGGATGCGCCGGCTGCTCCGCCCCTTCTTCCTGCTGCAGAATTCCTCCATGATGAA
GAAGACCCTGAAGTGTATACGGTGGTCGCTACCAGAAATGGCCAGTGTCGGGCTGCTGCTGGCCATCCAC
CTGTGTCTCTTCACCATCATTGGGATGCTGCTGTTCACCATTGGTGAGAAGGATGAAGCACAGGACCAGG
AGAGGCTGGCCTACTTCCGGAACCTTCCAGAAGCACTGACCTCACTCCTAGTGCTGCTGACGACTTCCAA
CAACCCTGATGTGATGATCCCTGCGTATACTCAGAACCGGGCCTTTGCTCTCTTCTTCATAGTCTTCACC
TTGATAGGAAGCTTGTTTCTGATGAACCTGCTGACAGCCATCATCTATAATCAGTTCCGTGGCTACCTAA
TGAAATCTCTACAGACCTCACTGTTCCGGCGGCGACTGGGGGCCCGTGCAGCCTACGAAGTCCTCGCCTC
CAGGGCCGGGCCAGCTGGAACCACCCCTGAGTTAGTTGGGGTGAATCCTGAGACCTTCCTGCCAGTGCTT
CAGAAAACGCAGCTGAACAAAACCCACAAACAAGCCATTATGCAGAAGGTGCAGTCCTATGAAGGCCGCC
CAATGCTGGCTGATGAGTTCCAGAAACTCTTCGATGAGGTTGACAAAGGTGTGGCCAAAGAGCGCCCGCT
GAAGCCCCAATACCAGTCTCCATTTCTGCAGACTGCCCAGTTCATCTTTAGCCACCACTACTTCGACTAC
CTGGGGAACCTCGTCGCTCTGGGAAACCTCTTGTCTATTTGTGTGTTCCTGGTGCTGGACTCAGACCTGC
TGCCTGGGGAACGTGATGACTTTGTCCTGGGGATTCTTGACTACATCTTCATCTTGTACTACCTGCTGGA
GCTGCTGTTTAAGGTGTTCGCGCTGGGCCTGCCAGGCTACCTGTCCTACCATAGCAATGTGTTTGATGGC
CTCCTCACCATCATCCTGCTGGTTTCGGAGATTTGCACCCTGGCTGTATACCGATTGCCGCACTCAGGAT
GGAAGCCAGAGCAGTATGGCCCACTGTCGCTCTGGGACATGACACGACTGATGAACACACTGATTGTGTT
TCGCTTCCTGCGCATCATCCCCAACATAAAGCCAATGGCTGAGGTAGCCAACACCATCCTGGGCCTAATC
CCTAACTTGAGGGCATTTGGTGGGATCCTGGTGGTGGCATACTATGTGTTTGCCATGATCGGGATCAACC
TGTTCCGAGGTGTCATCGTGCCTCCTGGAAACAGCAGCCTGGTTCCCGACAACAACTCAGCTGTATGTGG
AAGCTTTGAGCAGCTAGGTTACTGGCCCAACAACTTTGACGACTTTGCTGCTGCTTTGATCACACTGTGG
AACGTGATGGTGGTGAACAATTGGCAGGTGATCCTGGAAGCCTATAAGCGCTACGCAGGCCCGTGGTCAA
TGGTGTATTTTGTGCTATGGTGGCTGGTGTCCTCTGTTATCTGGATCAACCTGTTTCTGGCTCTTCTTCT
GGAGAACTTTCTCCACAGATGGGACCCTCAAGGTCATAAGCAGCTCCTTGTTGGGACCAAGCAGATGTCT
GTGGAGCTCATGTTCAGGGATATCCTAGAAGAGCCCAAGGAGGAGGAACTGATGGAGAAGCTACACAAAC
ACCCACACTTGCATCTGTGCAGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR225477 protein sequence
Red=Cloning site Green=Tags(s)

MAAEEQPLLGRGRGSGQVHSGAAADQELCIDQAVVFIEDAIKYRSIYHRMDAGSLWLYRWYYSNVCQRVL
GFIIFLILILAFVEVPSSFTKTADVRYRSQPWQPPCGLTETIEAFCLLAFLVDLSVKGYLVGQAQLQQNL
WLLAYFMVLVVSVVDWIVSLSLACEEPLRMRRLLRPFFLLQNSSMMKKTLKCIRWSLPEMASVGLLLAIH
LCLFTIIGMLLFTIGEKDEAQDQERLAYFRNLPEALTSLLVLLTTSNNPDVMIPAYTQNRAFALFFIVFT
LIGSLFLMNLLTAIIYNQFRGYLMKSLQTSLFRRRLGARAAYEVLASRAGPAGTTPELVGVNPETFLPVL
QKTQLNKTHKQAIMQKVQSYEGRPMLADEFQKLFDEVDKGVAKERPLKPQYQSPFLQTAQFIFSHHYFDY
LGNLVALGNLLSICVFLVLDSDLLPGERDDFVLGILDYIFILYYLLELLFKVFALGLPGYLSYHSNVFDG
LLTIILLVSEICTLAVYRLPHSGWKPEQYGPLSLWDMTRLMNTLIVFRFLRIIPNIKPMAEVANTILGLI
PNLRAFGGILVVAYYVFAMIGINLFRGVIVPPGNSSLVPDNNSAVCGSFEQLGYWPNNFDDFAAALITLW
NVMVVNNWQVILEAYKRYAGPWSMVYFVLWWLVSSVIWINLFLALLLENFLHRWDPQGHKQLLVGTKQMS
VELMFRDILEEPKEEELMEKLHKHPHLHLCR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_146206
ORF Size 2193 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_146206.2, NM_146206.3, NM_146206.4, NM_146206.5, NP_666318.2
RefSeq Size 2909 bp
RefSeq ORF 2196 bp
Locus ID 233979
UniProt ID Q8BWC0
Cytogenetics 7 F5
MW 83.5 kDa
Summary Nicotinic acid adenine dinucleotide phosphate (NAADP) receptor that may function as one of the major voltage-gated Ca(2+) channels (VDCC) across the lysosomal membrane. Involved in smooth muscle contraction.UniProtKB/Swiss-Prot Function
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"NM_146206" in other vectors (4)
SKU Description Size Price
MC221200 Tpcn2 (untagged) - Mouse two pore segment channel 2 (Tpcn2), (10ug) 10 ug
$670.00
MG225477 Tpcn2 (tGFP-tagged) - Mouse two pore segment channel 2 (Tpcn2), (10ug) 10 ug
$870.00
MR225477L3 Lenti ORF clone of Tpcn2 (Myc-DDK-tagged) - Mouse two pore segment channel 2 (Tpcn2) 10 ug
$970.00
MR225477L4 Lenti ORF clone of Tpcn2 (mGFP-tagged) - Mouse two pore segment channel 2 (Tpcn2) 10 ug
$970.00

Plasmid Map for MR225477

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR225477
Circular map for MR225477

file-alt Citations

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