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Ebf1 (NM_007897) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR225312
Ebf1 (Myc-DDK-tagged) - Mouse early B-cell factor 1 (Ebf1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $826.00
5 Days*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ebf1
Synonyms Ebf; O/E-1; OE-1; Olf-1; Olf1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR225312 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTTGGGATCCAGGAAAGCATCCAACGGAGTGGAAGCAGTATGAAGGAAGAGCCGCTGGGCAGCGGCA
TGAACGCGGTGCGGACGTGGATGCAGGGCGCCGGGGTGCTGGACGCCAACACAGCGGCGCAGAGCGGGGT
GGGTCTGGCCCGGGCTCACTTTGAGAAGCAGCCGCCTTCTAACCTGCGGAAATCCAACTTCTTCCACTTC
GTCCTGGCCCTCTACGACAGACAGGGCCAGCCCGTGGAGATTGAGAGGACTGCCTTTGTGGGGTTCGTGG
AGAAGGAAAAAGAAGCCAACAGCGAAAAGACCAATAATGGGATCCACTACCGGCTCCAGCTCCTCTACAG
CAATGGGATACGGACAGAACAGGATTTCTATGTGCGCCTCATCGACTCCATGACAAAACAAGCCATAGTG
TATGAAGGCCAAGACAAGAACCCTGAAATGTGCCGAGTATTGCTCACACACGAGATCATGTGCAGCCGCT
GTTGTGACAAGAAAAGCTGTGGCAACCGAAATGAGACTCCCTCAGATCCAGTGATAATTGACAGGTTCTT
CCTGAAGTTTTTCCTTAAATGCAACCAAAATTGCCTAAAGAATGCAGGAAACCCACGTGACATGCGGAGA
TTCCAGGTCGTGGTGTCTACCACAGTCAACGTGGATGGCCATGTCCTGGCAGTCTCTGATAACATGTTTG
TCCACAATAACTCCAAGCACGGGCGGAGGGCTCGGAGGCTTGACCCCTCGGAAGGTACGCCCTCTTATCT
GGAACATGCTACTCCCTGTATCAAAGCCATCAGCCCGAGTGAAGGATGGACGACGGGAGGCGCGACTGTG
ATCATCATAGGGGACAATTTCTTTGATGGGTTACAGGTCATATTCGGTACCATGCTGGTCTGGAGTGAGT
TGATAACTCCTCATGCCATCCGAGTTCAGACACCTCCTCGGCACATCCCTGGTGTGGTGGAAGTCACACT
GTCGTACAAGTCCAAGCAGTTCTGCAAAGGGACACCAGGCAGATTCATCTACACAGCACTCAATGAACCC
ACCATCGACTACGGCTTCCAGAGGTTACAGAAGGTCATTCCTCGGCATCCTGGTGACCCAGAGCGCTTGC
CAAAGGAAGTGATCCTTAAAAGAGCTGCAGATCTGGTTGAAGCCCTGTATGGGATGCCCCACAACAACCA
GGAGATTATCCTGAAGAGAGCTGCCGACATTGCAGAGGCTCTGTACAGTGTCCCTCGGAACCACAACCAG
CTCCCAGCCCTTGCTAACACTTCGGTCCATGCAGGGATGATGGGTGTGAACTCCTTCAGTGGACAACTGG
CTGTGAATGTCTCGGAGGCATCACAAGCCACCAATCAAGGTTTCACCCGCAACTCAAGCAGCGTGTCACC
ACATGGCTATGTGCCGAGCACCACCCCACAGCAAACCAACTATAACTCAGTCACCACAAGCATGAATGGC
TACGGCTCAGCCGCCATGTCCAATTTGGGCGGCTCCCCAACCTTCCTCAATGGCTCAGCTGCCAACTCAC
CCTATGCCATTGTGCCATCCAGCCCCACCATGGCCTCATCTACAAGCCTCCCCTCCAACTGCAGTAGCTC
CTCTGGCATCTTCTCCTTCTCACCAGCCAACATGGTCTCAGCAGTGAAACAGAAGAGTGCTTTCGCACCA
GTTGTCAGACCCCAGACGTCCCCTCCTCCCACCTGCACCAGCACCAACGGGAACAGCCTGCAAGCGATAT
CTGGCATGATTGTCCCTCCCATG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR225312 protein sequence
Red=Cloning site Green=Tags(s)

MFGIQESIQRSGSSMKEEPLGSGMNAVRTWMQGAGVLDANTAAQSGVGLARAHFEKQPPSNLRKSNFFHF
VLALYDRQGQPVEIERTAFVGFVEKEKEANSEKTNNGIHYRLQLLYSNGIRTEQDFYVRLIDSMTKQAIV
YEGQDKNPEMCRVLLTHEIMCSRCCDKKSCGNRNETPSDPVIIDRFFLKFFLKCNQNCLKNAGNPRDMRR
FQVVVSTTVNVDGHVLAVSDNMFVHNNSKHGRRARRLDPSEGTPSYLEHATPCIKAISPSEGWTTGGATV
IIIGDNFFDGLQVIFGTMLVWSELITPHAIRVQTPPRHIPGVVEVTLSYKSKQFCKGTPGRFIYTALNEP
TIDYGFQRLQKVIPRHPGDPERLPKEVILKRAADLVEALYGMPHNNQEIILKRAADIAEALYSVPRNHNQ
LPALANTSVHAGMMGVNSFSGQLAVNVSEASQATNQGFTRNSSSVSPHGYVPSTTPQQTNYNSVTTSMNG
YGSAAMSNLGGSPTFLNGSAANSPYAIVPSSPTMASSTSLPSNCSSSSGIFSFSPANMVSAVKQKSAFAP
VVRPQTSPPPTCTSTNGNSLQAISGMIVPPM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007897
ORF Size 1773 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007897.1
RefSeq Size 5203 bp
RefSeq ORF 1776 bp
Locus ID 13591
UniProt ID Q07802
Cytogenetics 11 26.45 cM
MW 64.5 kDa
Summary Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'.UniProtKB/Swiss-Prot Function
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"NM_007897" in other vectors (6)
SKU Description Size Price
MC219382 Ebf1 (untagged) - Mouse early B-cell factor 1 (Ebf1), (10ug) 10 ug
$881.00
MG225312 Ebf1 (tGFP-tagged) - Mouse early B-cell factor 1 (Ebf1), (10ug) 10 ug
$1,026.00
MR225312L1 Lenti ORF clone of Ebf1 (Myc-DDK-tagged) - Mouse early B-cell factor 1 (Ebf1) 10 ug
$1,126.00
MR225312L2 Lenti ORF clone of Ebf1 (mGFP-tagged) - Mouse early B-cell factor 1 (Ebf1) 10 ug
$1,126.00
MR225312L3 Lenti ORF clone of Ebf1 (Myc-DDK-tagged) - Mouse early B-cell factor 1 (Ebf1) 10 ug
$1,126.00
MR225312L4 Lenti ORF clone of Ebf1 (mGFP-tagged) - Mouse early B-cell factor 1 (Ebf1) 10 ug
$1,126.00

Plasmid Map for MR225312

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR225312
Circular map for MR225312

file-alt Citations

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