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Ebf1 (NM_007897) Mouse Untagged Clone

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SKU
MC219382
Ebf1 (untagged) - Mouse early B-cell factor 1 (Ebf1), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $881.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Ebf1
Synonyms Ebf; O/E-1; OE-1; Olf-1; Olf1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC219382 representing NM_007897
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTTGGGATCCAGGAAAGCATCCAACGGAGTGGAAGCAGTATGAAGGAAGAGCCGCTGGGCAGCGGCA
TGAACGCGGTGCGGACGTGGATGCAGGGCGCCGGGGTGCTGGACGCCAACACAGCGGCGCAGAGCGGGGT
GGGTCTGGCCCGGGCTCACTTTGAGAAGCAGCCGCCTTCTAACCTGCGGAAATCCAACTTCTTCCACTTC
GTCCTGGCCCTCTACGACAGACAGGGCCAGCCCGTGGAGATTGAGAGGACGGCCTTTGTGGGGTTCGTGG
AGAAGGAAAAAGAAGCCAACAGCGAAAAGACCAATAATGGGATCCACTACCGGCTCCAGCTCCTCTACAG
CAATGGGATACGGACAGAACAGGATTTCTATGTGCGCCTCATCGACTCCATGACAAAACAAGCCATAGTG
TATGAAGGCCAAGACAAGAACCCTGAAATGTGCCGAGTATTGCTCACACACGAGATCATGTGCAGCCGCT
GTTGTGACAAGAAAAGCTGTGGCAACCGAAATGAGACTCCCTCAGATCCAGTGATAATTGACAGGTTCTT
CCTGAAGTTTTTCCTTAAATGCAACCAAAATTGCCTAAAGAATGCAGGAAACCCACGTGACATGCGGAGA
TTCCAGGTCGTGGTGTCTACCACAGTCAACGTGGATGGCCATGTCCTGGCAGTCTCTGATAACATGTTTG
TCCACAATAACTCCAAGCACGGGCGGAGGGCTCGGAGGCTTGACCCCTCGGAAGGTACGCCCTCTTATCT
GGAACATGCTACTCCCTGTATCAAAGCCATCAGCCCGAGTGAAGGATGGACGACGGGAGGCGCGACTGTG
ATCATCATAGGGGACAATTTCTTTGATGGGTTACAGGTCATATTCGGTACCATGCTGGTCTGGAGTGAGT
TGATAACTCCTCATGCCATCCGAGTTCAGACACCTCCTCGGCACATCCCTGGTGTGGTGGAAGTCACACT
GTCGTACAAGTCCAAGCAGTTCTGCAAAGGGACACCAGGCAGATTCATCTACACAGCACTCAATGAACCC
ACCATCGACTACGGCTTCCAGAGGTTACAGAAGGTCATTCCTCGGCATCCTGGTGACCCAGAGCGCTTGC
CAAAGGAAGTGATCCTTAAAAGAGCTGCAGATCTGGTTGAAGCCCTGTATGGGATGCCCCACAACAACCA
GGAGATTATCCTGAAGAGAGCTGCCGACATTGCAGAGGCTCTGTACAGTGTCCCTCGGAACCACAACCAG
CTCCCAGCCCTTGCTAACACTTCGGTCCATGCAGGGATGATGGGTGTGAACTCCTTCAGTGGACAACTGG
CTGTGAATGTCTCGGAGGCATCACAAGCCACCAATCAAGGTTTCACCCGCAACTCAAGCAGCGTGTCACC
ACATGGCTATGTGCCGAGCACCACCCCACAGCAAACCAACTATAACTCAGTCACCACAAGCATGAATGGC
TACGGCTCAGCCGCCATGTCCAATTTGGGCGGCTCCCCAACCTTCCTCAATGGCTCAGCTGCCAACTCAC
CCTATGCCATTGTGCCATCCAGCCCCACCATGGCCTCATCTACAAGCCTCCCCTCCAACTGCAGTAGCTC
CTCTGGCATCTTCTCCTTCTCACCAGCCAACATGGTCTCAGCAGTGAAACAGAAGAGTGCTTTCGCACCA
GTTGTCAGACCCCAGACGTCCCCTCCTCCCACCTGCACCAGCACCAACGGGAACAGCCTGCAAGCGATAT
CTGGCATGATTGTCCCTCCCATGTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_007897
Insert Size 1776 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007897.3, NP_031923.1
RefSeq Size 5203 bp
RefSeq ORF 1776 bp
Locus ID 13591
UniProt ID Q07802
Cytogenetics 11 26.45 cM
Summary Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (2) uses an alternate splice site in the central coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_007897" in other vectors (6)
SKU Description Size Price
MG225312 Ebf1 (tGFP-tagged) - Mouse early B-cell factor 1 (Ebf1), (10ug) 10 ug
$1,026.00
MR225312 Ebf1 (Myc-DDK-tagged) - Mouse early B-cell factor 1 (Ebf1) 10 ug
$826.00
MR225312L1 Lenti ORF clone of Ebf1 (Myc-DDK-tagged) - Mouse early B-cell factor 1 (Ebf1) 10 ug
$1,126.00
MR225312L2 Lenti ORF clone of Ebf1 (mGFP-tagged) - Mouse early B-cell factor 1 (Ebf1) 10 ug
$1,126.00
MR225312L3 Lenti ORF clone of Ebf1 (Myc-DDK-tagged) - Mouse early B-cell factor 1 (Ebf1) 10 ug
$1,126.00
MR225312L4 Lenti ORF clone of Ebf1 (mGFP-tagged) - Mouse early B-cell factor 1 (Ebf1) 10 ug
$1,126.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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