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Ankrd42 (NM_028665) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR225102
Ankrd42 (Myc-DDK-tagged) - Mouse ankyrin repeat domain 42 (Ankrd42)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $480.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ankrd42
Synonyms 4931426M20; 4933417L02Rik; Ikbn
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR225102 representing NM_028665
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTGGTGTGGCTAATCCAGGCCCCTCCAAGTCCCGTAGGGAGACTGCAGACTCAAGTTCCAGGAAGA
AGGTACATTTCAGTAGCATACATGATGCGGTCCGGGCTGGAGATGTCAAGCAGCTGTCAGACATAGTGGA
GCGTGGTGCCAACCTTAATGAAGTGGATGCTCTCCATCAGTTTACTCCTTTACATTGGGCAGCACATTCT
GGAAGTTTAGAGTGCCTTCACTGGCTACTCTGGAGTGGTGCTGATGCCACACAGACAACTACAAGGGGAT
GGACAGCGGCTCACATAGCTGCAATCCGGGGTCAGGATGCCTGCTTACAGGCTCTTATAATCAATGGAGC
CAACCTAGCAACTCAAGATGATCGTGGATGCACCCCACTACACCTTGCTGCTACTCATGGACACTCTTTT
TCTTTACAAATAATGCTGCGAAGTGGAGTGGATCCCAGCGTGACTGACAAGAGAGAGTGGAAACCTGTGC
ATTATGCATCTTTTCATGGGCGGCTTGGTTGCTTGCAACTTCTTGTCAAATGGGGATGTGGCATCGAAGA
CGTGGACTACAATGGAAACCTCCCGGTTCATTTAGCAGCCATGGAAGGCCACCTCCATTGTTTGAAATTC
CTACTGAGTAGAATGAACAGTGCAACACAAGCCTTAAAAGCCTTCAATGATAATGGAGAAAATGTATTGG
ACCTGGCCCAGCGGTTTTTGAAACAGAATGTTGTAGAGTTTATTCAGGGGGCTCAGTATGAAGGAAGCCA
TCCAGATGATCATGATGATTTAGCCTTTCCAGGTCATGTGGCTGCCTTTAAGGGTGACTTAGAAGTGCTT
AAGAAACTGATAGGAGACGGGGTAATCAATCTGAATGAACGCGATGATAATGGATCAACTCCCATGCATA
AAGCTGCTGGACAGGGCCACATTGATTGTTTGCAGTGGTTAATTGAAATGGGAGCCGAAAGCAATATTAC
CAACAAAGCAGGAGAGACCCCCAGTGATGTGGCTAAGAGATTTGCCCATTTGGCTGCAGTGAAACTCTTG
GAGGGGCTACAGAAATATGAAATAGATGACATTGAGAGTGATAAAGATCATATAAATTTTTTTACAAGGC
ATGGTGTTGAAGGAAGCACCGATGCCAAGGATGACTTATGTCTGAGTGAATCAGACAAAGCAAATGCCAG
AATGAGAGCTCATAAGAAAATTGTAGAATTGAGACAACTTCTTGAAATCGCTGAGAGCAACTTTAAACAC
TTGGGTGGGATAACCGAAGAGGATTTAAAGCAGAAGAAAGAACAGCTGGAGTCTAAGAAGACCATCAACG
AGCTGCAGGGCCAGCTGGCCTATGAGCGACTCCGCAGGGAAAAGTTAGAGTGCCAGCTGGATGAGTATCG
AGTGGAAGTGGATCAACTCAAGGAAACGCTGCAGAAAATCCAGGTCACCAGTGTCGCGGCTATGGAAGAT
GACTCGTGTGAGTCTAGCAAAGAGAAGCGGCGAGTGAAAAAAAAAGTGTCACCTGGGGGTGTGTTTGTGA
GAAGGTAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR225102 representing NM_028665
Red=Cloning site Green=Tags(s)

MPGVANPGPSKSRRETADSSSRKKVHFSSIHDAVRAGDVKQLSDIVERGANLNEVDALHQFTPLHWAAHS
GSLECLHWLLWSGADATQTTTRGWTAAHIAAIRGQDACLQALIINGANLATQDDRGCTPLHLAATHGHSF
SLQIMLRSGVDPSVTDKREWKPVHYASFHGRLGCLQLLVKWGCGIEDVDYNGNLPVHLAAMEGHLHCLKF
LLSRMNSATQALKAFNDNGENVLDLAQRFLKQNVVEFIQGAQYEGSHPDDHDDLAFPGHVAAFKGDLEVL
KKLIGDGVINLNERDDNGSTPMHKAAGQGHIDCLQWLIEMGAESNITNKAGETPSDVAKRFAHLAAVKLL
EGLQKYEIDDIESDKDHINFFTRHGVEGSTDAKDDLCLSESDKANARMRAHKKIVELRQLLEIAESNFKH
LGGITEEDLKQKKEQLESKKTINELQGQLAYERLRREKLECQLDEYRVEVDQLKETLQKIQVTSVAAMED
DSCESSKEKRRVKKKVSPGGVFVRRY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028665
ORF Size 1548 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028665.4, NP_082941.2
RefSeq Size 2801 bp
RefSeq ORF 1551 bp
Locus ID 73845
UniProt ID Q3V096
Cytogenetics 7 E1
MW 57.3 kDa
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"NM_028665" in other vectors (4)
SKU Description Size Price
MC217324 Ankrd42 (untagged) - Mouse ankyrin repeat domain 42 (Ankrd42), (10ug) 10 ug
$529.00
MG225102 Ankrd42 (tGFP-tagged) - Mouse ankyrin repeat domain 42 (Ankrd42), (10ug) 10 ug
$680.00
MR225102L3 Lenti ORF clone of Ankrd42 (Myc-DDK-tagged) - Mouse ankyrin repeat domain 42 (Ankrd42) 10 ug
$780.00
MR225102L4 Lenti ORF clone of Ankrd42 (mGFP-tagged) - Mouse ankyrin repeat domain 42 (Ankrd42) 10 ug
$780.00

Plasmid Map for MR225102

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR225102
Circular map for MR225102

file-alt Citations

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