Deaf1 (NM_016874) Mouse Tagged ORF Clone

SKU MR224847

Deaf1 (Myc-DDK-tagged) - Mouse deformed epidermal autoregulatory factor 1 (Drosophila) (Deaf1), transcript variant 1

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Deaf1
• Synonyms: AU042387; C230009B13Rik; NUDR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224847 representing NM_016874; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGAGGACTCGGACTCGGCGGCAAAACAGCTGGGCCTGGCCGAGGCGGCAGCTGTGGCGGCCGCTGCCG; CGGTGGCGGCGGCCGCCGCGGCCGCAGCGGAAAGCGAGGCGGAGGAGCCGGTGTTAAGCAGAGACGAGGA; CTCGGAGGAGGACGCAGACTCCGAGGCGGAGCGCGAGACGCGGCGGGTCACCGCTGTGGCGGTGATGGCG; GCCGAATCTGGGCACATGGATATGGGTACCGAGGCTCTGCCCAGCCCCGATGAGGCCGCCGCCGCCGCCG; CCGCCTTCGCAGAAGTGACCACAGTGACTGTGGCCAACGTGGGGTCCTCTGCAGATAACGTCTTCACAAC; ATCAGTGGCAAACGCAGCATCGATATCAGGACATGTCCTGTCTGGTAGGACAGCCCTGCAGATCGGTGAC; AGCCTGAACACTGAAAAAGCCACACTAATAGTTGTACACACAGATGGGAGCATTGTGGAGACCACTGGGC; TGAAGGGCCCAGCAGCACCTCTCACTCCAGGTCCCCAGTCTCCTCCTACCCCACTGGCACCTGGCCAGGA; GAAAGGTGGTACCAAGTACAACTGGGACCCTTCGGTGTATGACAGCGAGTTGCCTGTGCGCTGTCGGAAC; ATCAGTGGCACGCTCTACAAGAGTAGGCTCGGCTCAGGTGGCCGGGGCCGGTGTATCAAGCAGGGAGAAA; ACTGGTACAGCCCAACTGAGTTTGAAGCTATGGCAGGAAGAGCCAGCAGCAAGGACTGGAAGAGAAGCAT; CCGCTATGCTGGCAGACCCCTGCAGTGCCTCATTCAGGATGGTATTTTGAACCCTCACGCTGCCTCCTGT; ACCTGTGCCGCCTGTTGTGATGACATGACTCTGAGTGGCCCTGTCAGGCTCTTCGTCCCTTATAAAAGGC; GCAAGAAAGAGAATGAGCTGCCCACAACTCCAGTGAAGAAGGATTCCCCCAAGAACATCACCCTGCTTCC; TGCCACGGCGGCCACCACCTTCACTGTGACACCCTCAGGACAGATCACTACCTCTGGAGCACTGACCTTT; GACAGAGCATCCACTGTAGAGGCCACTGCTGTCATCTCTGAGAGCCCAGCCCAAGGTGATGTCTTTGCAG; GAGCCACAGTGCAAGAGGCAGGTGTGCAGCCTCCCTGCAGGGTTGGCCACCCTGAACCCCACTACCCTGG; CTATCAGGACAGCTGCCAGATTGCCCCGTTTCCAGAAGCTGCATTGCCAACATCACACCCCAAAATTGTC; CTGACATCGCTGCCCGCATTGGCCGTGCCACCGTCCACCCCCACCAAAGCTGTCTCTCCCACCGTGGTCA; GTGGGCTGGAGATGTCAGAACATCGGAGCTGGCTGTACCTGGAAGAGATGGTCAACTCCCTACTCAACAC; AGCTCAGCAGCTGAAGACGCTGTTTGAACAAGCCAAGCAGGCGAGCTCTTGCAGGGAAGCTGCTGTGACC; CAGGCGAGAATGCAGGTTGATACAGAGAGGAAAGAGCAGTCATGTGTCAACTGCGGCCGGGAGGCCATGA; GTGAGTGTACCGGCTGCCACAAGGTTAACTACTGCTCTACGTTCTGCCAGCGCAAGGACTGGAAAGACCA; TCAGCATGTGTGTGGCCAGTCAGCGTCTGTCACTGTCCAGGCTGATGACGTCCATGTTGAAGAAAGTGTG; ATAGAAAAAGTTGCTGTT; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224847 representing NM_016874; Red=Cloning site Green=Tags(s); ; MEDSDSAAKQLGLAEAAAVAAAAAVAAAAAAAAESEAEEPVLSRDEDSEEDADSEAERETRRVTAVAVMA; AESGHMDMGTEALPSPDEAAAAAAAFAEVTTVTVANVGSSADNVFTTSVANAASISGHVLSGRTALQIGD; SLNTEKATLIVVHTDGSIVETTGLKGPAAPLTPGPQSPPTPLAPGQEKGGTKYNWDPSVYDSELPVRCRN; ISGTLYKSRLGSGGRGRCIKQGENWYSPTEFEAMAGRASSKDWKRSIRYAGRPLQCLIQDGILNPHAASC; TCAACCDDMTLSGPVRLFVPYKRRKKENELPTTPVKKDSPKNITLLPATAATTFTVTPSGQITTSGALTF; DRASTVEATAVISESPAQGDVFAGATVQEAGVQPPCRVGHPEPHYPGYQDSCQIAPFPEAALPTSHPKIV; LTSLPALAVPPSTPTKAVSPTVVSGLEMSEHRSWLYLEEMVNSLLNTAQQLKTLFEQAKQASSCREAAVT; QARMQVDTERKEQSCVNCGREAMSECTGCHKVNYCSTFCQRKDWKDHQHVCGQSASVTVQADDVHVEESV; IEKVAV; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016874
• ORF Size: 1698 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_016874.3, NP_058570.1
• RefSeq Size: 2192 bp
• RefSeq ORF: 1701 bp
• Locus ID: 54006
• UniProt ID: Q9Z1T5
• Cytogenetics: 7 F5
• MW: 60.1 kDa
• Summary: Transcription factor that binds to sequence with multiple copies of 5'-TTC[CG]G-3' present in its own promoter and that of the HNRPA2B1 gene. Down-regulates transcription of these genes. Binds to the retinoic acid response element (RARE) 5'-AGGGTTCACCGAAAGTTCA-3'. Activates the proenkephalin gene independently of promoter binding, probably through protein-protein interaction (By similarity). Regulates epithelial cell proliferation and side-branching in the mammary gland. Required for neural tube closure and skeletal patterning. Controls the expression of peripheral tissue antigens in pancreatic lymph nodes. Isoform 1 displays greater transcriptional activity than isoform 2. Isoform 2 may inhibit transcriptional activity of isoform 1 by interacting with it and retaining it in the cytoplasm. Transcriptional activator of EIF4G3 (By similarity). May also involved in behavior (PubMed:24726472).[UniProtKB/Swiss-Prot Function]