P2rx6 (NM_011028) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR224683
P2rx6 (Myc-DDK-tagged) - Mouse purinergic receptor P2X, ligand-gated ion channel, 6 (P2rx6), transcript variant 1
$289.00 $457.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol P2rx6
Synonyms P2rxl1; P2x6; P2xm
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR224683 representing NM_011028
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGTTGCAGCCAGCAGGAACTGGGAACATGGCTTCTGCAGCCGCAGCAGCACTCGTGAGCTGGGGGT
TTCTGGATTACAAGACGGAGAAATATGTGCTAACCAGGAACTGTCGGGTGGGCGTCTCCCAGAGGCTGCT
GCAGCTGGCAGTGGTAGTCTACGTGATAGGGTGGGCCCTCTTGGCCAAAAAAGGCTACCAGGAACGGGAC
TTGGCCCCTCAGACTTCTGTCATCACCAAACTCAAGGGGGTTTCTGTAACCCAGGTTAAGGAGCTGGAGA
ACCGGCTGTGGGACGTGGCTGACTTTGTGAAGCCATCACAGGGAGAGAACGTGTTCTTCCTGGTAACCAA
CTTCCTCGTGACACCAGCTCAAGTCCAGGGCAGATGTCCAGAGCATCCTTCCGTTCCTCTGGCTAACTGC
TGGGCTGATGAAGACTGCCCCGAAGGGGAGACGGGGACATACAGCCATGGCATAAAAACTGGTCAGTGTG
TGGTGTTCAACGGAACCCACAGGACCTGTGAGATCTGGAGCTGGTGCCCAGTGGAGAGTGGTGCTGTGCC
CAGGAAGCCCCTGCTAGCTCAGGCCAAGAACTTCACACTCTTCATCAAAAATACTGTAACCTTCAGCAAG
TTCAACTTCTCCAGATCCAATGCCTTATTAACCTGGGACAACACATATTTCAAGCATTGTCTCTATGATC
CACTCTCCAGTCCGTACTGCCCAGTGTTCCGCATCGGGGACCTTGTGGCCATGGCTGGAGGGGACTTTGA
GGACCTGGCCTTGCTGGGTGGTGCTGTGGGCATCAGCATTCACTGGGATTGCAACCTGGACACGAAAGGC
TCTGACTGCTGTCCCCAGTACTCCTTCCAGCTGCAGCAGAAAGGGTACAACTTCAGGACAGCCAATCACT
GGTGGGCAGCCTCAGGCGTGGAGACCCGGAGCCTGCTCAAGCTCTATGGAATCCGTTTTGACATTCTTGT
CACTGGGCAGGCAGGGAAGTTCGCGCTCATCCCTACGGCCATCACAGTGGGCACAGGAGCGGCTTGGCTG
GGCATGGTCACCTTCCTCTGTGACCTGCTGCTACTGTATGTGGATAGAGAGGCCGGTTTCTACTGGAGAA
CCAAATATGAAGAGGCAAGAGCCCCAAAGACGACTACCAACAGTTCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR224683 representing NM_011028
Red=Cloning site Green=Tags(s)

MQLQPAGTGNMASAAAAALVSWGFLDYKTEKYVLTRNCRVGVSQRLLQLAVVVYVIGWALLAKKGYQERD
LAPQTSVITKLKGVSVTQVKELENRLWDVADFVKPSQGENVFFLVTNFLVTPAQVQGRCPEHPSVPLANC
WADEDCPEGETGTYSHGIKTGQCVVFNGTHRTCEIWSWCPVESGAVPRKPLLAQAKNFTLFIKNTVTFSK
FNFSRSNALLTWDNTYFKHCLYDPLSSPYCPVFRIGDLVAMAGGDFEDLALLGGAVGISIHWDCNLDTKG
SDCCPQYSFQLQQKGYNFRTANHWWAASGVETRSLLKLYGIRFDILVTGQAGKFALIPTAITVGTGAAWL
GMVTFLCDLLLLYVDREAGFYWRTKYEEARAPKTTTNSS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_011028
ORF Size 1167 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_011028.2, NP_035158.2
RefSeq Size 2362 bp
RefSeq ORF 1170 bp
Locus ID 18440
UniProt ID O54803
Cytogenetics 16 A3
MW 43.5 kDa
Summary Receptor for ATP that acts as a ligand-gated ion channel.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_011028" in other vectors (4)
SKU Description Size Price
MC209135 P2rx6 (untagged) - Mouse purinergic receptor P2X, ligand-gated ion channel, 6 (P2rx6), transcript variant 1, (10ug) 10 ug
$503.00
MG224683 P2rx6 (tGFP-tagged) - Mouse purinergic receptor P2X ligand-gated ion channel 6 (P2rx6) transcript variant 1, (10ug) 10 ug
$657.00
MR224683L3 Lenti ORF clone of P2rx6 (Myc-DDK-tagged) - Mouse purinergic receptor P2X, ligand-gated ion channel, 6 (P2rx6), transcript variant 1 10 ug
$757.00
MR224683L4 Lenti ORF clone of P2rx6 (mGFP-tagged) - Mouse purinergic receptor P2X, ligand-gated ion channel, 6 (P2rx6), transcript variant 1 10 ug
$757.00

Plasmid Map for MR224683

Download sequence for

Cloning scheme for MR224683

Circular map for MR224683

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