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Lgr6 (NM_001033409) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR224646
Lgr6 (Myc-DDK-tagged) - Mouse leucine-rich repeat-containing G protein-coupled receptor 6 (Lgr6)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $886.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Lgr6 or NM_001033409"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Lgr6
Synonyms A530037C04Rik; D830026M09
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR224646 representing NM_001033409
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCACAGCCCGCCTGGGCTCCTGGCGCTGTGGCTTTGCGCTGTGCTGTGCGCATCGGCGCGCGGGGGCA
GCGACCCCCAGCCTGGCCCGGGGCGTCCCGCCTGCCCGGCTCCCTGCCACTGCCAGGAGGACGGCATCAT
GCTGTCCGCTGACTGCTCCGAGCTCGGGCTCTCAGTGGTGCCTGCGGACCTGGACCCCCTGACGGCTTAC
CTAGACCTCAGTATGAACAACCTCACGGAGCTTCAGCCGGGTCTCTTCCACCACCTGCGCTTCCTGGAGG
AGCTGCGGCTCTCAGGGAACCACCTCTCACACATCCCGGGACAGGCATTCTCCGGCCTCCACAGCCTCAA
AATTCTAATGCTGCAGAGCAACCAGCTCCGTGGGATCCCAGCAGAGGCACTATGGGAGCTGCCCAGCCTG
CAGTCGCTGCGCCTAGATGCTAATCTCATCTCCCTGGTCCCTGAGAGAAGCTTTGAGGGGCTCTCCTCCC
TCCGCCACCTCTGGCTGGATGACAATGCACTCACTGAGATCCCCGTCAGAGCTCTCAACAACCTTCCTGC
CCTACAGGCCATGACCTTGGCTCTCAACCATATCCGCCACATCCCTGACTATGCCTTCCAGAACCTCACC
AGTCTTGTGGTGCTGCATCTACATAACAACCGCATCCAGCATGTGGGGACCCACAGCTTCGAGGGGCTGC
ACAATCTGGAGACACTAGACCTGAACTATAATGAGCTGCAGGAGTTCCCCTTGGCTATCCGGACCCTGGG
CAGGCTGCAGGAATTGGGTTTCCATAACAACAACATCAAGGCTATCCCAGAGAAAGCCTTCATGGGCAAC
CCTCTCCTGCAGACAATACATTTTTATGACAACCCAATCCAGTTTGTGGGAAGGTCAGCATTCCAGTACC
TGTCTAAACTGCATACGCTATCTTTGAATGGTGCCACTGATATCCAAGAGTTCCCAGACCTCAAAGGCAC
CACTAGCCTGGAGATCCTGACCCTGACCCGTGCGGGCATCAGACTGCTCCCACCGGGAGTGTGCCAACAG
CTGCCTAGGCTCCGAATCCTGGAGCTGTCTCATAATCAGATCGAGGAGTTACCCAGCCTGCACAGATGTC
AGAAGCTGGAGGAAATTGGCCTCCGACATAACAGGATCAAGGAAATTGGTGCAGATACCTTCAGCCAGCT
GGGCTCCTTGCAAGCTTTAGACCTGAGTTGGAATGCCATCCGTGCCATCCACCCTGAGGCTTTCTCAACC
CTTCGATCCTTGGTTAAGCTGGACCTGACTGACAACCAGCTGACCACACTGCCCCTGGCTGGGCTGGGAG
GCCTGATGCACCTGAAGCTCAAAGGGAACTTGGCCCTGTCTCAGGCCTTCTCCAAGGACAGTTTCCCAAA
ACTGAGGATCCTGGAGGTGCCCTACGCCTACCAGTGCTGTGCCTACGGCATCTGTGCCAGCTTCTTCAAG
ACCTCTGGGCAGTGGCAGGCCGAGGACTTTCATCCAGAAGAAGAGGAGGCACCAAAGAGGCCCCTGGGTC
TCCTTGCTGGACAAGCTGAGAACCACTATGACCTAGACCTGGATGAGCTCCAGATGGGGACAGAGGACTC
AAAGCCAAACCCCAGTGTCCAGTGCAGCCCTGTTCCAGGCCCCTTCAAGCCCTGCGAGCACCTCTTTGAG
AGCTGGGGCATCCGCCTTGCTGTGTGGGCCATCGTGTTACTCTCCGTACTCTGTAACGGGCTGGTGCTGC
TGACAGTCTTTGCCAGCGGACCCAGCCCGCTGTCCCCCGTCAAGCTTGTGGTGGGTGCGATGGCAGGCGC
CAACGCCCTGACGGGCATTTCCTGTGGTCTCCTGGCCTCGGTGGACGCCTTGACCTATGGTCAGTTCGCT
GAGTATGGAGCCCGCTGGGAGAGCGGTCTGGGCTGCCAGGCTACGGGCTTCCTGGCTGTCCTGGGTTCAG
AGGCGTCGGTGCTGCTGCTCACACTGGCGGCGGTGCAGTGCAGCATCTCTGTGACCTGCGTCCGAGCCTA
CGGGAAGGCGCCGTCGCCTGGCAGCGTCCGCGCAGGCGCACTGGGATGCCTGGCGCTGGCCGGGCTGGCC
GCAGCACTGCCGCTGGCCTCGGTGGGAGAGTATGGCGCCTCCCCACTCTGCCTGCCCTACGCCCCACCCG
AGGGCCGGCCGGCCGCCCTGGGCTTCGCTGTAGCCCTGGTGATGATGAACTCGCTCTGCTTCCTGGTGGT
GGCCGGCGCCTACATCAAGCTCTACTGTGACCTGCCACGGGGTGACTTTGAGGCCGTGTGGGACTGCGCC
ATGGTGCGCCACGTGGCCTGGCTCATCTTTGCAGATGGCCTCCTCTACTGCCCCGTGGCCTTCCTCAGCT
TTGCCTCTATGCTGGGCCTCTTCCCTGTCACCCCCGAGGCTGTCAAGTCAGTCCTTCTGGTGGTGCTGCC
TCTGCCTGCCTGCCTCAACCCACTGCTCTACCTGCTCTTCAACCCTCACTTCCGGGATGACCTTCGGCGG
CTCTGGCCAAGCCCTCGGTCCCCAGGGCCCCTAGCCTACGCTGCAGCCGGTGAGCTGGAGAAGAGCTCCT
GCGACTCCACCCAAGCGCTGGTGGCTTTCTCAGATGTGGATCTTATTCTGGAAGCTTCTGAGGCTGGGCA
GCCTCCTGGGCTAGAGACCTATGGCTTCCCTTCAGTGACCCTCATCTCCCGACATCAGCCGGGGGCCACC
AGGCTGGAGGGAAACCATTTTATAGAGTCTGATGGAACCAAGTTTGGGAACCCACAACCTCCCATGAAGG
GAGAACTGCTGCTGAAGGCAGAGGGAGCCACTTTGGCAGGCTGTGGCTCTTCCGTGGGTGGAGCCCTCTG
GCCCTCTGGCTCTCTCTTTGCCTCTCACTTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR224646 protein sequence
Red=Cloning site Green=Tags(s)

MHSPPGLLALWLCAVLCASARGGSDPQPGPGRPACPAPCHCQEDGIMLSADCSELGLSVVPADLDPLTAY
LDLSMNNLTELQPGLFHHLRFLEELRLSGNHLSHIPGQAFSGLHSLKILMLQSNQLRGIPAEALWELPSL
QSLRLDANLISLVPERSFEGLSSLRHLWLDDNALTEIPVRALNNLPALQAMTLALNHIRHIPDYAFQNLT
SLVVLHLHNNRIQHVGTHSFEGLHNLETLDLNYNELQEFPLAIRTLGRLQELGFHNNNIKAIPEKAFMGN
PLLQTIHFYDNPIQFVGRSAFQYLSKLHTLSLNGATDIQEFPDLKGTTSLEILTLTRAGIRLLPPGVCQQ
LPRLRILELSHNQIEELPSLHRCQKLEEIGLRHNRIKEIGADTFSQLGSLQALDLSWNAIRAIHPEAFST
LRSLVKLDLTDNQLTTLPLAGLGGLMHLKLKGNLALSQAFSKDSFPKLRILEVPYAYQCCAYGICASFFK
TSGQWQAEDFHPEEEEAPKRPLGLLAGQAENHYDLDLDELQMGTEDSKPNPSVQCSPVPGPFKPCEHLFE
SWGIRLAVWAIVLLSVLCNGLVLLTVFASGPSPLSPVKLVVGAMAGANALTGISCGLLASVDALTYGQFA
EYGARWESGLGCQATGFLAVLGSEASVLLLTLAAVQCSISVTCVRAYGKAPSPGSVRAGALGCLALAGLA
AALPLASVGEYGASPLCLPYAPPEGRPAALGFAVALVMMNSLCFLVVAGAYIKLYCDLPRGDFEAVWDCA
MVRHVAWLIFADGLLYCPVAFLSFASMLGLFPVTPEAVKSVLLVVLPLPACLNPLLYLLFNPHFRDDLRR
LWPSPRSPGPLAYAAAGELEKSSCDSTQALVAFSDVDLILEASEAGQPPGLETYGFPSVTLISRHQPGAT
RLEGNHFIESDGTKFGNPQPPMKGELLLKAEGATLAGCGSSVGGALWPSGSLFASHL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001033409
ORF Size 2901 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001033409.1, NM_001033409.2, NM_001033409.3, NP_001028581.1, BC141210.1
RefSeq Size 3623 bp
RefSeq ORF 2904 bp
Locus ID 329252
UniProt ID Q3UVD5
Cytogenetics 1 E4
MW 104.3 kDa
Summary Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a marker of multipotent stem cells in the epidermis. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal (By similarity).UniProtKB/Swiss-Prot Function
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Resources
"NM_001033409" in other vectors (4)
SKU Description Size Price
MC222887 Lgr6 (untagged) - Mouse leucine-rich repeat-containing G protein-coupled receptor 6 (Lgr6), (10ug) 10 ug
$886.00
MG224646 Lgr6 (tGFP-tagged) - Mouse leucine-rich repeat-containing G protein-coupled receptor 6 (Lgr6), (10ug) 10 ug
$1,086.00
MR224646L3 Lenti ORF clone of Lgr6 (Myc-DDK-tagged) - Mouse leucine-rich repeat-containing G protein-coupled receptor 6 (Lgr6) 10 ug
$1,186.00
MR224646L4 Lenti ORF clone of Lgr6 (mGFP-tagged) - Mouse leucine-rich repeat-containing G protein-coupled receptor 6 (Lgr6) 10 ug
$1,186.00

Plasmid Map for MR224646

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR224646
Circular map for MR224646

file-alt Citations

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