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Emid1 (NM_080595) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR224527
Emid1 (Myc-DDK-tagged) - Mouse EMI domain containing 1 (Emid1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Emid1
Synonyms AW122071; CO-5; Emu1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR224527 representing NM_080595
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCGGCCCGCGGGCCTGGACGCTGCTCTGCCTCGGGCTCCTGCTCCCGGGAGGCGGCGCCGCGTGGA
GCGTCCCGGGCGCCCGGTTCTCGGGTCGCAGGAACTGGTGCTCCTACGTGGTGACCCGGACTGTCTCCTG
CCACGTGCAAAATGGTACCTACCTCCAGCGAGTGCTACAGAACTGCCCGTGGCCCATGGGCTGCCCTGGG
AACAGCTACAGAACCGTGGTGAGACCCTTGTACAAAGTGACGTACAAGACAGTGACGGCCCGAGAATGGA
GGTGCTGCCCTGGACATTCAGGGGTGACCTGTGAGGAAGGCTCCCCTGGCCTCCTGGAGCCCACGTGGAC
GGACAGCGGCATGCGGCGGATGGCAGTGAGGCCCACAGCCCTCTCAGGTTGTCTCAACTGCAGCAAAGTG
TCTGAGCTGACAGAGAGGCTGAAGGCGCTGGAGGCCAAGGTGGCGGTGCTGAGTGTGACAGAGCAGACAG
TGCCCTCAGTCCCAGCCACCCCTGAGGACTCTGCCCTGCTCTGGGGCTCCCCGGCTGCCCGGGGCAGCCC
TGGAGATGGAAGCCTGCAGGATAGACTTGATTCTTGGGGGCTTCCTGGGCCCACAGGCCCCAAGGGAGGC
ACCGACAGCCAGAGCCCAGTGAGGATTAGAGGCCCGCCAGGCCCACAGGGACCCCCAGGACGCCCTGGCC
AAACAGGAGCTGCAGGCACCCCTGGAAAGATGGGACCTCCAGGGCCTCCAGGTCCACCTGGACCCCCAGG
GCCTCCAGCCCCTGTAGGGCCACCCTATGGCCAGGTCTCTCTGCATGGGGATCCACTGCTGTCTAATACC
TTCACTGAGATGGGCAGCCACTGGCCCCAAGGACCCACTGGGCCCCCAGGTCCTCCAGGCCCCCCAGGGC
CCATGGGTCCTCCTGGACTTCCTGGCCCCATGGGTGCCCCTGGGAGTCCTGGACACATGGGAATCCCAGG
CCCTTCTGGACCCAAAGGAACCTCTGGCCACCCAGGAGAGAAGGGAGAGAGAGGACTGCCTGGGGAACCT
GGCCCCCAGGGTTTGATGGGGGTTCCGGGAGAACCTGGCCCCAAAGGAGACCCTGGTGAGAAGAGCCACT
GGGGGGAGGGGTTGCACCAACTACGCGAGGCTTTGAAGATTTTAGCTGAGAGGGTTTTAATCTTGGAAAC
AATGATTGGGCTCTATGAACCAGACTTGGGATCCGGGGCAGGCCCTGATGGCACAGGTACCCCCAGCCTC
CTTCGAGGCAAGAGGGGAGGACACCCAACCAACTACCCGATCATCACCCCCAGGAGACGCAGCGAGAGGA
GC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR224527 representing NM_080595
Red=Cloning site Green=Tags(s)

MGGPRAWTLLCLGLLLPGGGAAWSVPGARFSGRRNWCSYVVTRTVSCHVQNGTYLQRVLQNCPWPMGCPG
NSYRTVVRPLYKVTYKTVTAREWRCCPGHSGVTCEEGSPGLLEPTWTDSGMRRMAVRPTALSGCLNCSKV
SELTERLKALEAKVAVLSVTEQTVPSVPATPEDSALLWGSPAARGSPGDGSLQDRLDSWGLPGPTGPKGG
TDSQSPVRIRGPPGPQGPPGRPGQTGAAGTPGKMGPPGPPGPPGPPGPPAPVGPPYGQVSLHGDPLLSNT
FTEMGSHWPQGPTGPPGPPGPPGPMGPPGLPGPMGAPGSPGHMGIPGPSGPKGTSGHPGEKGERGLPGEP
GPQGLMGVPGEPGPKGDPGEKSHWGEGLHQLREALKILAERVLILETMIGLYEPDLGSGAGPDGTGTPSL
LRGKRGGHPTNYPIITPRRRSERS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_080595
ORF Size 1332 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_080595.3
RefSeq Size 1911 bp
RefSeq ORF 1335 bp
Locus ID 140703
UniProt ID Q91VF5
Cytogenetics 11 A1
MW 46.1 kDa
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Documents
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"NM_080595" in other vectors (4)
SKU Description Size Price
MC215863 Emid1 (untagged) - Mouse EMI domain containing 1 (Emid1), (10ug) 10 ug
$503.00
MG224527 Emid1 (tGFP-tagged) - Mouse EMI domain containing 1 (Emid1), (10ug) 10 ug
$657.00
MR224527L3 Lenti ORF clone of Emid1 (Myc-DDK-tagged) - Mouse EMI domain containing 1 (Emid1) 10 ug
$757.00
MR224527L4 Lenti ORF clone of Emid1 (mGFP-tagged) - Mouse EMI domain containing 1 (Emid1) 10 ug
$757.00

Plasmid Map for MR224527

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR224527
Circular map for MR224527

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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