Srrd (NM_027323) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR224393
Srrd (Myc-DDK-tagged) - Mouse SRR1 domain containing (Srrd)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Srrd
Synonyms 2810002G02Rik; Srr1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR224393 representing NM_027323
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGGCGGCTCTCGAGCCCTGGAGCGCGGTGGCTCCGCGGAGGAGGAAGCGCGCGGCGGGAAGGC
GACCGCGGCCGGGCGAGGGGCCCCGGGCCGAGCCGGAGGCGGACGGCGAAGCGGTGCTACGCCGCCTGCG
GGAGGCCGAGGAGGACCTGCGGATCTCTGATTTCTGCAGTTCGGCACTGGAAACCATCACCGAGTGTCTT
AGGAAACAGTTGGAGCAACTGCAGCCCCTAACAGAAGCCCTTGGAAGGCTGCACCTTGGCTCATCGTTGC
CTAGTGCGTCACAGGAGCCCTTGGCTTCAAGCGCCTCCCATGTGAAGTGTGTGTGCTACGGTCTTGGGAC
CTTTGCCTCCTGCCCCACTGCTCGGATCCAGCTTGCTTTTATGCTTCTCTTTCTAGAGAAGTGCCAGGTC
CCCAGAAGTCACTGCTGGGTCTATGACCCTCTGTTCAGTCAGACTGAGGTTTCCGTTCTCACCTCCCTCG
GCGTGACTGTCCTCAGTGAGAATGAGGAAGGCAAGCGCAGTGTCCAGGGCCAGCCCACTGTCTTCTACAT
GCCACACTGTGGGACAGCTCTATACAACAACCTGCTGTGGAGCAACTGGTCTGCAGACGCCCTGTCCAGG
GTGCTCATCATCGGGAACAGCTTCCGAGGCCTTGAGGAAAGGTTGTTGGCGAGGATTCTGCAGGAAAATT
ACCCCTACATTGCAAAGGTGTCTGACAGAATAGCTGGGCCGGGATTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR224393 representing NM_027323
Red=Cloning site Green=Tags(s)

MAAAALEPWSAVAPRRRKRAAGRRPRPGEGPRAEPEADGEAVLRRLREAEEDLRISDFCSSALETITECL
RKQLEQLQPLTEALGRLHLGSSLPSASQEPLASSASHVKCVCYGLGTFASCPTARIQLAFMLLFLEKCQV
PRSHCWVYDPLFSQTEVSVLTSLGVTVLSENEEGKRSVQGQPTVFYMPHCGTALYNNLLWSNWSADALSR
VLIIGNSFRGLEERLLARILQENYPYIAKVSDRIAGPGF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027323
ORF Size 747 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027323.2, NP_081599.2
RefSeq Size 1134 bp
RefSeq ORF 750 bp
Locus ID 70118
UniProt ID Q8K2M3
Cytogenetics 5 F
MW 27.5 kDa
Summary Plays a role in the regulation of heme biosynthesis and in the regulation of the expression of core clock genes.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_027323" in other vectors (4)
SKU Description Size Price
MC211042 Srrd (untagged) - Mouse SRR1 domain containing (Srrd), (10ug) 10 ug
$330.00
MG224393 Srrd (tGFP-tagged) - Mouse SRR1 domain containing (Srrd), (10ug) 10 ug
$500.00
MR224393L3 Lenti ORF clone of Srrd (Myc-DDK-tagged) - Mouse SRR1 domain containing (Srrd) 10 ug
$600.00
MR224393L4 Lenti ORF clone of Srrd (mGFP-tagged) - Mouse SRR1 domain containing (Srrd) 10 ug
$600.00

Plasmid Map for MR224393

Download sequence for

Cloning scheme for MR224393

Circular map for MR224393

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