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Ankrd61 (NM_025732) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR224252
Ankrd61 (Myc-DDK-tagged) - Mouse ankyrin repeat domain 61 (Ankrd61)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ankrd61
Synonyms 4921520G13Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR224252 representing NM_025732
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAAACATAACCAAGAGAGGAAGCAGAGACCTGGTGGCTGACGGTGCCACGTTGGTGGAGGAGAGCC
TGGCCACGACCCTGCACTCTAAACTGTATGAGGCCATCATCAAGGAAGACTGTAACACCATCAAGACACT
TCTCAGAAACCACCCTGTCAACCAGCCCCTGACCATCCTGGCCAACTCCACCCGTTACAGACTACTGAGC
CAGCAGACACAGCCTATCTTTCCCATCCATCTGGCCGCCGAGTACCGCAAGCCACAAAGTTTGCTTTGCT
TGTTACAACACGGTGCTGACCCAGAAGTAAGGGACGCCCAAGGCTTCACTACCCTTCACCTGATGCTGCT
TAACTGGCCAGCCTCTTCTACCACTTGGTCCAAACCCAGCACTCAGATCCAAAAGATCCTGATGGACATT
CAGAACAACGCTGTCTTGTGTCTGTGCATTTTGTGTGACCACGGAGCTCAAGTTAATGCCCGGGTAGACA
ACAGCAACAAGCATTCAGCCCTCCACCTGGCCATAATACACGGGACCTACCCAGTTCTCTCCTTTTTGGC
TCAAAATGGTGCCCAGGTCAATGCTACTAATGAATCCAGCATGACGCCCCTCCACATGGCTGCAGACATA
CTGAACAAAAACATGATAGAGACACTCATTGCCTTTGGGGCCAATGTGAACTGTGCCATCTCATCTACCG
GAAACACAGCTCTGAAGCTGGCCGTGTGTACTGCATCGAGCAAAGTCGGCCGACTGCTGGCAGCGGGGGT
GGGCTGCATCCGTCTGCTGCTAAATAACGGAGCCCAGGTCAATGCCCAGGACCATGAAGGCCAGACAGCT
CTGCACGAGGCATGCTTTGGAGGCAGAGAAGCGATCATCAGTCTCTTGCTGGAATTTGAGGCAAACGTTA
ACATTTTAACAAGAAATGGGGAGTCTCCCATTTACATGTACCTTCAGCGCAGTTCCAACATCAGAGACAG
ATCGCTTCTGGCCAGACTACTTTACCGCACTTATCCTTTAAGACTGAGCAATAAACAGGGGATTCTACCA
GCAGGAATCATGCTGCCAGAATACCAGCTCCTAAGGGAAACCCTAGTAAAGTTATCAAAAAAACCTTTAA
CCCTCGAGGCCATCTGTAAGAGAAGCATCAGGAATGTTTATGGGGAGAAGTACAAATTCCACCTCGAGAA
GCTCCTCCCGGCAAAGCTCTGGAACTCCATATATGGCATCTATGACCTCACCTACCTGCTCAAAGGAGAG
CCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR224252 representing NM_025732
Red=Cloning site Green=Tags(s)

MGNITKRGSRDLVADGATLVEESLATTLHSKLYEAIIKEDCNTIKTLLRNHPVNQPLTILANSTRYRLLS
QQTQPIFPIHLAAEYRKPQSLLCLLQHGADPEVRDAQGFTTLHLMLLNWPASSTTWSKPSTQIQKILMDI
QNNAVLCLCILCDHGAQVNARVDNSNKHSALHLAIIHGTYPVLSFLAQNGAQVNATNESSMTPLHMAADI
LNKNMIETLIAFGANVNCAISSTGNTALKLAVCTASSKVGRLLAAGVGCIRLLLNNGAQVNAQDHEGQTA
LHEACFGGREAIISLLLEFEANVNILTRNGESPIYMYLQRSSNIRDRSLLARLLYRTYPLRLSNKQGILP
AGIMLPEYQLLRETLVKLSKKPLTLEAICKRSIRNVYGEKYKFHLEKLLPAKLWNSIYGIYDLTYLLKGE
P

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025732
ORF Size 1263 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025732.4, NP_080008.1
RefSeq Size 1496 bp
RefSeq ORF 1266 bp
Locus ID 66729
UniProt ID Q9CQM6
Cytogenetics 5 G2
MW 46.6 kDa
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"NM_025732" in other vectors (4)
SKU Description Size Price
MC210425 Ankrd61 (untagged) - Mouse ankyrin repeat domain 61 (Ankrd61), (10ug) 10 ug
$503.00
MG224252 Ankrd61 (tGFP-tagged) - Mouse ankyrin repeat domain 61 (Ankrd61), (10ug) 10 ug
$657.00
MR224252L3 Lenti ORF clone of Ankrd61 (Myc-DDK-tagged) - Mouse ankyrin repeat domain 61 (Ankrd61) 10 ug
$757.00
MR224252L4 Lenti ORF clone of Ankrd61 (mGFP-tagged) - Mouse ankyrin repeat domain 61 (Ankrd61) 10 ug
$757.00

Plasmid Map for MR224252

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR224252
Circular map for MR224252

file-alt Citations

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