Rad23a (NM_009010) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR224081
Rad23a (Myc-DDK-tagged) - Mouse RAD23a homolog (S. cerevisiae) (Rad23a)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rad23a
Synonyms 2310040P19Rik; AL024030; HR23A; mHR23A
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR224081 representing NM_009010
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGTCACCATCACCCTTAAAACGTTGCAGCAGCAGACCTTCAAGATCCGCATGGAACCTGACGAGA
CGGTAAAGGTGCTGAAGGAGAAGATAGAAGCTGAGAAGGGTAGAGATGCTTTCCCCGTGGCTGGACAGAA
ACTCATCTATGCTGGCAAGATCTTGAGTGACGATGTTCCCATCAAGGAATACCATATAGATGAGAAGAAC
TTTGTGGTTGTCATGGTGACCAAGGCCAAAGCTGGCCAGGGTATCCCGGCACCCCCAGAGGCCTCACCCA
CTGCTGTCCCGGAGCCCTCCACACCTTTCCCTCCAGTCCTGGCATCAGGCATGTCTCATCCCCCACCTAC
CAGCAGAGAGGACAAGAGCCCATCAGAGGAGTCAACCACCACAACATCTCCAGAATCCATTTCTGGCTCT
GTTCCCTCTTCAGGTAGCAGCGGGCGAGAGGAAGACGCAGCTTCCACATTAGTGACTGGCTCTGAATATG
AGACGATGCTGACTGAGATCATGTCCATGGGCTACGAGCGGGAGCGGGTTGTGGCCGCATTGAGGGCCAG
CTACAACAACCCCCACCGAGCTGTGGAGTACTTGCTCACGGGAATTCCAGGAAGCCCTGAGCCTGAACAT
GGTTCTGTCCAGGAAAGCCAGGCGCCTGAGCAGCCGGCCACAGAAGCAGGGGAGAACCCCCTGGAGTTCC
TGCGGGACCAGCCTCAGTTCCAGAACATGCGGCAAGTGATTCAACAGAACCCAGCGCTCCTGCCTGCTCT
GCTCCAGCAGCTGGGTCAGGAAAACCCTCAGCTCCTGCAGCAAATTAGCCGTCACCAGGAGCAGTTCATC
CAGATGTTGAATGAGCCTCCCGGGGAGCTGGCGGACATCTCTGATGTAGAGGGGGAGGTTGGTGCCATAG
GTGAGGAGGCCCCACAGATGAACTATATCCAGGTGACACCGCAGGAGAAGGAGGCTATAGAAAGGCTGAA
GGCACTGGGCTTCCCAGAGAGCCTGGTGATCCAGGCCTACTTCGCGTGTGAAAAAAATGAGAATTTGGCT
GCCAACTTCCTCCTGAGTCAGAACTTTGATGATGAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR224081 representing NM_009010
Red=Cloning site Green=Tags(s)

MAVTITLKTLQQQTFKIRMEPDETVKVLKEKIEAEKGRDAFPVAGQKLIYAGKILSDDVPIKEYHIDEKN
FVVVMVTKAKAGQGIPAPPEASPTAVPEPSTPFPPVLASGMSHPPPTSREDKSPSEESTTTTSPESISGS
VPSSGSSGREEDAASTLVTGSEYETMLTEIMSMGYERERVVAALRASYNNPHRAVEYLLTGIPGSPEPEH
GSVQESQAPEQPATEAGENPLEFLRDQPQFQNMRQVIQQNPALLPALLQQLGQENPQLLQQISRHQEQFI
QMLNEPPGELADISDVEGEVGAIGEEAPQMNYIQVTPQEKEAIERLKALGFPESLVIQAYFACEKNENLA
ANFLLSQNFDDE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009010
ORF Size 1086 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009010.6
RefSeq Size 2074 bp
RefSeq ORF 1089 bp
Locus ID 19358
Cytogenetics 8 C2
MW 40.1 kDa
Summary Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to 'Lys-48'-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to 'Lys-63'-linked polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_009010" in other vectors (4)
SKU Description Size Price
MC209281 Rad23a (untagged) - Mouse RAD23a homolog (S. cerevisiae) (Rad23a), (10ug) 10 ug
$503.00
MG224081 Rad23a (tGFP-tagged) - Mouse RAD23a homolog (S. cerevisiae) (Rad23a), (10ug) 10 ug
$657.00
MR224081L3 Lenti ORF clone of Rad23a (Myc-DDK-tagged) - Mouse RAD23a homolog (S. cerevisiae) (Rad23a) 10 ug
$757.00
MR224081L4 Lenti ORF clone of Rad23a (mGFP-tagged) - Mouse RAD23a homolog (S. cerevisiae) (Rad23a) 10 ug
$757.00

Plasmid Map for MR224081

Download sequence for

Cloning scheme for MR224081

Circular map for MR224081

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