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Mfsd8 (NM_028140) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR224019
Mfsd8 (Myc-DDK-tagged) - Mouse major facilitator superfamily domain containing 8 (Mfsd8)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $483.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Mfsd8 or NM_028140"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mfsd8
Synonyms 2810423E13Rik; AI836898; AV142426; Cln7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR224019 representing NM_028140
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGAACCTGGGAAGTGAGGCCGAGCGGGAGCCCCTCTTGGGCCCGGGATCACCCGGAAGCAGAGAAT
GGAGCGAGATAGAAACCCAAGAGCATTACAAGAGTAGATGGAAGTCTGTGAGGATTCTCTACCTTACCAT
GTTCCTCAGCAGTGTGGGTTTTTCTATTGTGATAATGTCCATCTGGCCATATCTTCAAAAGATTGATCAG
ACAGCTGATGCAAGTTTTTTGGGCTGGGTTATTGCTTCATATAGTCTTGGCCAAATGGTAGCTTCACCTC
TATTTGGCTTATGGTCTAATTATAGGCCGAGAAAAGAACCTCTTATCGTCTCCATCTCTATTTCGGTGGC
AGCCAACTGCCTATATGCGTATGTCCACGTGCCAGCTGCTCATAATAAATACTACATGTTGATTGCTCGT
GGATTGGTGGGATTTGGAGCAGGAAATGTTGCCGTTGTCCGATCATACATTGCTGGTGCTACTTCCCTTC
AGGAAAGAACAAATGCCATGGCTAATACAAGCACATGCCAAGCCCTGGGCTTTATTCTCGGTCCAGTTTT
TCAGACTTGCTTTGCACTCATTGGAGAAAAAGGTGTGACGTGGGACATCATCAAGCTGCAGGTCAACATG
TACACAGCACCAGTTTTACTTGCAGCCTTCCTGGGAATTCTAAACATTATTCTGATTCTTTTTATATTAA
GAGAGCATCGTGTGGATGACTTGGGACGTCAGTGTAAGAGTGTGAATTTTCAAGAAGAAAATACAGATGA
ACCTCAGATTCCTGAAGGAAGTATTGATCAAGTTGCTGTTGTGGCTACCAATATTGTGTTTTTTGTGGTT
TTATTTATCTTTGCTGTTTATGAAACAATCCTCACCCCACTAACACTGGATATGTACGCCTGGACCCAGG
AGCAGGCTGTGCTCTATGATGGAATCCTCCTTGTTGCTTTTGGAGTTGAGGCAGTTCTTGTCTTCATGGG
GGTTAAATTACTTTCCAAAAAGATTGGCGAGCGTGCTATTCTCCTGGGAGGATTTGTGGTTGTATGGGTC
GGCTTCTTTATCTTGTTACCCTGGGGAAATCAGTTTCCCAAAATACAGTGGGAAGATTTACACAACAGCT
CAACCCCTAATACTACATTTGGGGAAATAATTATTGGTCTTTGGAATTCTTCAAGAGAAGATCACAGTGA
ACAGCCAACTGGGTGCCCAATTGAACAGACCTGGTGCCTGTACACCCCAGTGATTCATCTGGCCCAGTTC
CTGACAGCTGCTGTGTTAATTGGAACAGGCTATCCAGCCTGCAGTGTCATGTCCTATACATTATACTCAA
AAGTTCTAGGACCCAAACCTCAGGGTATATACATGGGCTGGTTAACCACTTCTGGAAGTGCAGCACGGAT
TCTTGGGCCTGTGTTCATCAGCCATGTGTACACTTACTTGGGCCCACGATGGGCATTCAGCCTTGTGTGT
GGAATAGTAGTGCTCACCATCTTGCTAATAGGAGCTGTTTACAAAAGACTCGTTGCCTTTTCTGTCAGAT
ATATGAGGATCCAGGAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR224019 representing NM_028140
Red=Cloning site Green=Tags(s)

MANLGSEAEREPLLGPGSPGSREWSEIETQEHYKSRWKSVRILYLTMFLSSVGFSIVIMSIWPYLQKIDQ
TADASFLGWVIASYSLGQMVASPLFGLWSNYRPRKEPLIVSISISVAANCLYAYVHVPAAHNKYYMLIAR
GLVGFGAGNVAVVRSYIAGATSLQERTNAMANTSTCQALGFILGPVFQTCFALIGEKGVTWDIIKLQVNM
YTAPVLLAAFLGILNIILILFILREHRVDDLGRQCKSVNFQEENTDEPQIPEGSIDQVAVVATNIVFFVV
LFIFAVYETILTPLTLDMYAWTQEQAVLYDGILLVAFGVEAVLVFMGVKLLSKKIGERAILLGGFVVVWV
GFFILLPWGNQFPKIQWEDLHNSSTPNTTFGEIIIGLWNSSREDHSEQPTGCPIEQTWCLYTPVIHLAQF
LTAAVLIGTGYPACSVMSYTLYSKVLGPKPQGIYMGWLTTSGSAARILGPVFISHVYTYLGPRWAFSLVC
GIVVLTILLIGAVYKRLVAFSVRYMRIQE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028140
ORF Size 1557 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028140.5
RefSeq Size 3051 bp
RefSeq ORF 1560 bp
Locus ID 72175
UniProt ID Q8BH31
Cytogenetics 3 B
MW 58 kDa
Summary May be a carrier that transport small solutes by using chemiosmotic ion gradients.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_028140" in other vectors (4)
SKU Description Size Price
MC217321 Mfsd8 (untagged) - Mouse major facilitator superfamily domain containing 8 (Mfsd8), (10ug) 10 ug
$532.00
MG224019 Mfsd8 (tGFP-tagged) - Mouse major facilitator superfamily domain containing 8 (Mfsd8), (10ug) 10 ug
$683.00
MR224019L3 Lenti ORF clone of Mfsd8 (Myc-DDK-tagged) - Mouse major facilitator superfamily domain containing 8 (Mfsd8) 10 ug
$783.00
MR224019L4 Lenti ORF clone of Mfsd8 (mGFP-tagged) - Mouse major facilitator superfamily domain containing 8 (Mfsd8) 10 ug
$783.00

Plasmid Map for MR224019

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR224019
Circular map for MR224019

file-alt Citations

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