Lyg2 (NM_001033427) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR223980
Lyg2 (Myc-DDK-tagged) - Mouse lysozyme G-like 2 (Lyg2)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Lyg2
Synonyms Gm776
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR223980 representing NM_001033427
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGCCCTCGGTGGTGTTCTGGGGACTTATTGCTCTCGTTGGCACTGCCAAGGGCTCATATACTCACT
CTGTGCACTCCATGAACCCTCATGTGCACCCTCGTCTGTACCATGGCTGCTATGGGGACATCATGACCAT
GGAGACTTTTGGGGCCCCCTGCGACATAAATAATCTGATGAACTGTGGGATCCACGGTTCGGAAATGTTT
GCCGAGATGGATTTGAAAGCCATAAAACCTTACCGGATCCTCATCAAAGAAGTGGGGCAGAGACACTGTA
TTGACCCAGCCCTCATCGCTGCCATCATCTCCAGGGAAAGCCACGGCGGGGCTGTCCTGCAAAATGGCTG
GGACCATAAGGGGCAGAGGTTTGGCTTGATGCAGCTTGATAAAAATATGTACCATCCTATTGGTTCCTGG
GATAGCAAGGAACACCTTCTACAGTCTGTTGGGATTCTAACAGAAAGAATTAAGGCAATGAAGAGGAAAT
TTCCCACCTGGAATACAGCTCAGCAGCTGAAAGGTGGTCTGACTGCTTTTAAGTCGGGGATGGAAACTAT
TGTCACTCCTGCAGACATAGACGGTGACTTAGTGGATGATGTCCTTGCCCGGGCCAAATTCTATAAGAGA
CATGGCTTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR223980 representing NM_001033427
Red=Cloning site Green=Tags(s)

MVPSVVFWGLIALVGTAKGSYTHSVHSMNPHVHPRLYHGCYGDIMTMETFGAPCDINNLMNCGIHGSEMF
AEMDLKAIKPYRILIKEVGQRHCIDPALIAAIISRESHGGAVLQNGWDHKGQRFGLMQLDKNMYHPIGSW
DSKEHLLQSVGILTERIKAMKRKFPTWNTAQQLKGGLTAFKSGMETIVTPADIDGDLVDDVLARAKFYKR
HGF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001033427
ORF Size 639 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001033427.3, NP_001028599.1
RefSeq Size 2564 bp
RefSeq ORF 642 bp
Locus ID 332427
UniProt ID Q3V1I0
Cytogenetics 1 B
MW 24.2 kDa
Summary May act as a potent antibacterial protein that may play a role in the innate immunity.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001033427" in other vectors (4)
SKU Description Size Price
MC214613 Lyg2 (untagged) - Mouse lysozyme G-like 2 (Lyg2), (10ug) 10 ug
$330.00
MG223980 Lyg2 (tGFP-tagged) - Mouse lysozyme G-like 2 (Lyg2), (10ug) 10 ug
$500.00
MR223980L3 Lenti ORF clone of Lyg2 (Myc-DDK-tagged) - Mouse lysozyme G-like 2 (Lyg2) 10 ug
$600.00
MR223980L4 Lenti ORF clone of Lyg2 (mGFP-tagged) - Mouse lysozyme G-like 2 (Lyg2) 10 ug
$600.00

Plasmid Map for MR223980

Download sequence for

Cloning scheme for MR223980

Circular map for MR223980

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