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Hmbs (NM_013551) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR223896
Hmbs (Myc-DDK-tagged) - Mouse hydroxymethylbilane synthase (Hmbs), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $503.00
2 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Hmbs
Synonyms PBGD; T25658; Ups; Uros1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR223896 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCGGTAACGGCGGCGCGGCCACAACCGCGGAAGAAAACGGCTCAAAGATGAGGGTGATTCGAGTGG
GCACCCGTAAGAGCCAGCTGGCTCGCATACAGACCGACACTGTGGTGGCGATGCTGAAAGCCTTGTACCC
TGGCATACAGTTTGAAATCATTGCTATGTCCACCACGGGAGACAAGATTCTTGATACTGCACTCTCTAAG
ATTGGAGAGAAGAGCCTGTTTACCAAGGAGCTAGAAAACGCCCTGGAAAAAAACGAAGTGGACCTGGTCG
TTCACTCCCTGAAGGATGTGCCTACCATACTACCTCCTGGCTTTACTATTGGAGCCATCTGCAAACGGGA
AAACCCTTGTGATGCTGTTGTCTTTCACCCAAAGTTTATTGGAAAGACCCTGGAAACCTTGCCAGAGAAA
AGTGCCGTGGGAACCAGCTCTCTGAGGAGAGTGGCTCAGCTACAGAGAAAGTTCCCCCACCTGGAATTCA
AGAGTATTCGGGGAAACCTCAACACCCGCCTCCGGAAGCTGGATGAGCTGCAGGAATTCAGTGCCATCGT
CCTGGCTGTGGCTGGCCTACAGCGCATGGGCTGGCAGAACCGGGTGGGCCAGATTTTGCACCCAGAAGAA
TGCATGTATGCTGTGGGTCAGGGAGCCCTAGCCGTGGAAGTCCGAGCCAAGGACCAGGATATCTTGGACC
TAGTGAGTGTGTTGCACGATCCTGAAACTCTGCTTCGCTGCATTGCTGAAAGGGCTTTTCTGAGGCACCT
GGAAGGAGGCTGCAGCGTGCCCGTAGCAGTGCATACAGTGATGAAAGATGGGCAACTGTACCTGACTGGT
GGAGTCTGGAGTCTAGATGGCTCAGATAGCATGCAAGAGACTATGCAGGCCACCATCCAGGTCCCTGTTC
AGCAAGAAGATGGTCCAGAAGATGACCCACAACTGGTTGGAATCACTGCCCGTAACATTCCAAGAGGAGC
CCAGCTAGCTGCTGAGAACCTGGGCATCAGCCTGGCCAGCTTGCTGCTCAACAAAGGAGCCAAGAACATC
CTGGATGTTGCACGGCAGCTTAATGATGTGCGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR223896 protein sequence
Red=Cloning site Green=Tags(s)

MSGNGGAATTAEENGSKMRVIRVGTRKSQLARIQTDTVVAMLKALYPGIQFEIIAMSTTGDKILDTALSK
IGEKSLFTKELENALEKNEVDLVVHSLKDVPTILPPGFTIGAICKRENPCDAVVFHPKFIGKTLETLPEK
SAVGTSSLRRVAQLQRKFPHLEFKSIRGNLNTRLRKLDELQEFSAIVLAVAGLQRMGWQNRVGQILHPEE
CMYAVGQGALAVEVRAKDQDILDLVSVLHDPETLLRCIAERAFLRHLEGGCSVPVAVHTVMKDGQLYLTG
GVWSLDGSDSMQETMQATIQVPVQQEDGPEDDPQLVGITARNIPRGAQLAAENLGISLASLLLNKGAKNI
LDVARQLNDVR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_013551
ORF Size 1083 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013551.2, NP_038579.2
RefSeq Size 1611 bp
RefSeq ORF 1086 bp
Locus ID 15288
UniProt ID P22907
Cytogenetics 9 24.84 cM
MW 39.3 kDa
Summary Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_013551" in other vectors (4)
SKU Description Size Price
MC200084 Hmbs (untagged) - Mouse hydroxymethylbilane synthase (Hmbs), transcript variant 1, (10ug) 10 ug
$457.00
MG223896 Hmbs (tGFP-tagged) - Mouse hydroxymethylbilane synthase (Hmbs) transcript variant 1, (10ug) 10 ug
$703.00
MR223896L3 Lenti ORF clone of Hmbs (Myc-DDK-tagged) - Mouse hydroxymethylbilane synthase (Hmbs), transcript variant 1 10 ug
$803.00
MR223896L4 Lenti ORF clone of Hmbs (mGFP-tagged) - Mouse hydroxymethylbilane synthase (Hmbs), transcript variant 1 10 ug
$803.00

Plasmid Map for MR223896

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR223896
Circular map for MR223896

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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