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Hey2 (NM_013904) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR223872
Hey2 (Myc-DDK-tagged) - Mouse hairy/enhancer-of-split related with YRPW motif 2 (Hey2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Hey2
Synonyms bHLHb32; CHF1; Herp1; hesr2; Hrt2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR223872 representing NM_013904
Red=Cloning site Blue=ORF Green=Tags(s)

CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGCGCCCTTGTGAGGAAACGACCTCCGAAAGCGACCTGGACGAGACCATCGACGTGGGGAGCGAGA
ACAATTACCCTGGGCACGCTACAAGCTCAGTGATGAGGTCCAATTCACCGACAACTACCTCTCAGATTAT
GGCAAGAAAGAAAAGGAGAGGGATCATAGAAAAAAGGCGTCGGGATCGAATAAATAACAGTTTATCTGAA
TTGAGAAGACTAGTGCCAACAGCTTTTGAAAAACAAGGATCTGCCAAGTTAGAAAAGGCTGAAATATTGC
AAATGACAGTGGATCATTTGAAGATGCTCCAGGCTACAGGGGGTAAAGGCTACTTTGATGCCCATGCTCT
TGCCACAGACTTCATGAGCATTGGATTCCGAGAGTGCTTGACAGAAGTGGCTAGGTACCTAAGCTCAGTG
GAAGGCCTTGACCCGTCGGACCCACTACGCGTGCGCCTTGTCTCTCATCTCAGCACCTGTGCCTCCCAGC
GGGAGGCAGCAGTGATGACATCCTCCATGGCCCACCACCATCACCCCTTGCACCCTCACCACTGGGCAGC
TGCTTTCCACCATCTCCCCACAGCCCTGCTCCAGCCCAATGGACTCCACACATCAGAGTCAACCCCATGT
CGCCTATCCACATCTTCAGAAGTGCCTTCTGCTCATGGCTCTGCTCTCCTCACAGCAACGTTTGCCCATG
CAGATTCTGCTCTTCGGATGCCATCAGGGGGCACCGTTGCACCCTGCGTGCCACCTCTCTCCACCTCTCT
TCTGTCTCTTTCGGCCACTGTGCATGCCGCAGCTGCAGCAGCCACTGCAGCTGCACACAGCTTCCCTCTG
TCCTTCGCAGGGGCTTTTCCCATGCTCCCGTCCAATGCAGCGGCAGCAGCCGCTGTTGCTGCTGCAACAG
CAATCAGCCCACCCTTGTCGGTATCCGCAGCCTCCAGTCCTCAGCAGACAAGCACTGGGACAAACAATAA
ACCTTACCAACCCTGGGGGACAGAAGTTGGAGCCTTT

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_013904
ORF Size 1017 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013904.1, NP_038932.1
RefSeq Size 2549 bp
RefSeq ORF 1020 bp
Locus ID 15214
UniProt ID Q9QUS4
Cytogenetics 10 A4
MW 36.3 kDa
Summary Transcriptional repressor which functions as a downstream effector of Notch signaling in cardiovascular development. Specifically required for the Notch-induced endocardial epithelial to mesenchymal transition, which is itself criticial for cardiac valve and septum development. May be required in conjunction with HEY1 to specify arterial cell fate or identity. Promotes maintenance of neuronal precursor cells and glial versus neuronal fate specification. Binds preferentially to the canonical E box sequence 5'-CACGTG-3'. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6 and by the neuronal bHLH factors ASCL1/MASH1 and NEUROD4/MATH3.UniProtKB/Swiss-Prot Function
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"NM_013904" in other vectors (4)
SKU Description Size Price
MC208661 Hey2 (untagged) - Mouse hairy/enhancer-of-split related with YRPW motif 2 (Hey2), (10ug) 10 ug
$503.00
MG223872 Hey2 (tGFP-tagged) - Mouse hairy/enhancer-of-split related with YRPW motif 2 (Hey2), (10ug) 10 ug
$657.00
MR223872L3 Lenti ORF clone of Hey2 (Myc-DDK-tagged) - Mouse hairy/enhancer-of-split related with YRPW motif 2 (Hey2) 10 ug
$757.00
MR223872L4 Lenti ORF clone of Hey2 (mGFP-tagged) - Mouse hairy/enhancer-of-split related with YRPW motif 2 (Hey2) 10 ug
$757.00

Plasmid Map for MR223872

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR223872
Circular map for MR223872

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