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Tslp (NM_021367) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR223795
Tslp (Myc-DDK-tagged) - Mouse thymic stromal lymphopoietin (Tslp), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $225.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tslp
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR223795 representing NM_021367
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTTCTTCTCAGGAGCCTCTTCATCCTGCAAGTACTAGTACGGATGGGGCTAACTTACAACTTTTCTA
ACTGCAACTTCACGTCAATTACGAAAATATATTGTAACATAATTTTTCATGACCTGACTGGAGATTTGAA
AGGGGCTAAGTTCGAGCAAATCGAGGACTGTGAGAGCAAGCCAGCTTGTCTCCTGAAAATCGAGTATTAT
ACTCTCAATCCTATCCCTGGCTGCCCTTCACTCCCCGACAAAACATTTGCCCGGAGAACAAGAGAAGCCC
TCAATGACCACTGCCCAGGCTACCCTGAAACTGAGAGAAATGACGGTACTCAGGAAATGGCACAAGAAGT
CCAAAACATCTGTCTGAATCAAACCTCACAAATTCTAAGATTGTGGTATTCCTTCATGCAATCTCCAGAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR223795 representing NM_021367
Red=Cloning site Green=Tags(s)

MVLLRSLFILQVLVRMGLTYNFSNCNFTSITKIYCNIIFHDLTGDLKGAKFEQIEDCESKPACLLKIEYY
TLNPIPGCPSLPDKTFARRTREALNDHCPGYPETERNDGTQEMAQEVQNICLNQTSQILRLWYSFMQSPE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_021367
ORF Size 420 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_021367.1
RefSeq Size 1143 bp
RefSeq ORF 423 bp
Locus ID 53603
UniProt ID Q9JIE6
Cytogenetics 18 18.12 cM
MW 16.6 kDa
Summary Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells (By similarity).UniProtKB/Swiss-Prot Function
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"NM_021367" in other vectors (4)
SKU Description Size Price
MC209940 Tslp (untagged) - Mouse thymic stromal lymphopoietin (Tslp), transcript variant 1, (10ug) 10 ug
$225.00
MG223795 Tslp (tGFP-tagged) - Mouse thymic stromal lymphopoietin (Tslp) transcript variant 1, (10ug) 10 ug
$425.00
MR223795L3 Lenti ORF clone of Tslp (Myc-DDK-tagged) - Mouse thymic stromal lymphopoietin (Tslp), transcript variant 1 10 ug
$525.00
MR223795L4 Lenti ORF clone of Tslp (mGFP-tagged) - Mouse thymic stromal lymphopoietin (Tslp), transcript variant 1 10 ug
$525.00

Plasmid Map for MR223795

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR223795
Circular map for MR223795

file-alt Citations

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