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Extl1 (NM_019578) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR223710
Extl1 (Myc-DDK-tagged) - Mouse exostoses (multiple)-like 1 (Extl1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $614.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Extl1
Synonyms D430033M16Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR223710 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGTGGAGAAGAAAGTCCTTCTGGCTGGCGTTGTCAGCCTTCTGGCTCCTCCTCGTCCTCCTAGGAG
TCTTCCCCCTTCGCCTGGCAGTGTCGCCCGGTCCTCTTCCAGGGCGCTCCCAAGGCTGGCCCCGCTGGCT
GGATGCTGCCTTCTTACAGAGTTTCTCCCAGTCTGAGACGAACCCAGAAGATGTCGCACAGCTCCCTCGG
GTCTCTCGGGGAAGCAGCTGCACCTGGGGAGCCTGCTTTGACACCTCCAAGTGTAGGGGAAAGGTCCTCA
AGATCTTTGTGCACTCCCCGGCTGGTCCGACCTCTGAGGCTCAGCGCAGGATCCTGGATTCCTTGGAAGG
CTCCCGCTACTCTGCACTCAGCCCAGCAGACGCTTGCCTTCTCCTTTTTCTCCCCAGCCAGGATCGTAGA
GGAGCCTGCGGGCCCCTGCCTCCGAACTGGAACGGAGGCCGGAACCATCTGGTGTTGAGTCTCTACCCAG
CCCCTTGCACCAGGCTGGGACAGGCGATGGTAGCTGAGGCCAGCCCCTCCTCGGACATCTTCCGACCGGG
CTTCGATATAGCGCTCCCGTATCTCCCCGAAGCTCACCCATTGCGAGGTGGGGCTCCCGGCAAACTGCAG
CAGCACAGTCCCCAACCTGGGGCCACCTTACTGGCGGTAGCAGAGGAGAAAGGCAGGTGGCGTATCACAA
GCACCCACGCCTCAGCCTGCCTCTGGGACAGGCACTGTGAGCAAGACCCTGGACCCCAGCAGACCTACCC
AGGAGAGACCCTTCCCAATGCCACCTTCTGCCTCATCCCTGGTCACCGATCTGCCACCTCCTGCTTTCTC
CAAGCCCTTCAGGCCGGCTGCATCCCCGTGCTCCTCAGCCCCCGCTGGGAGCTGCCTTTCTCTGAAGTCA
TCGACTGGACCAAGGCAGCCATCATTGCTGATGAGAGACTCCCACTGCAGGTCCTGGCTGCCCTCCGTGA
AATGCTCCCTTCGAGGGTCCTCGCCCTGCGCCAGCAGACCCAGTTTCTGTGGACCGCTTACTTCTCCTCG
GTGGAAAAAGTTATCCATACCACCCTAGAGGTTATTCAGGACCGGATCTGGGGTGCATCAGGTCACCCCT
CACTGATGTGGAATAGCCCCCCGGGAGCACTCTTGGCGCTGCCCACATTTTCCACGAGCCTTCAAGACTT
CCCCTTTTATCATCTGCAACTGGGCTCTGGCCCTGGGAGCAGTTTCAGCGCCGTGATCTGGGTGGGAGCC
TCTGGAGAGTCCCTGCTGAAGCTCATCCAGGAGGTGGCAGGTTCTCGGCACTGTGCCCAGATCCTGATTC
TCTGGAACAGTGAGAAGCCGCCCCCTGACAGGTGGCCCGAGACAGCAGTGCCCCTAACAGTCATCAAGGG
GCACAGGAAGGTCAGCAACCGGTTCTTCCCGTACAGCAACATCAGCACCAATGTTATCCTCAGCCTTGAT
GCTCAGAGCACTCTTTCTACCAGTGAGGTGGACTTTGCCTTTGTGGTGTGGCAGAGCTTTCCTGAGCGGA
TGGTGGGCTTTCTGTCCGGAAGCCACTTCTGGGATGAGGCCCAGGGTGGCTGGGGCTACCGTACTGGGAT
GACCAATGACTTCTCCATGGTCCTGACCACGGCTGCCTTCTACCACAGGTACTACCACACCCTCTTCACC
CACTCCCTGCCTAAGGCCCTGAGGACCATAGCTGATGAGACTCCCACCTGTGTTGACGTCCTGATGAACT
TCCTGGTAGCCACCGTCACCAAACTGCCCCCTATCAAGGTGCCTTATGGGAGGCAGCACCCGGAGGCTGT
GCCAATGGATTCTGGGGATCCACGACCAGTGCCGGAGCCTCAGCCCCTGGATCAGGACTGCATCAACCGT
TTAGCAGCAGGGTTCGGCCACATGCCTCTGGTTTCCTCTCAAGTGCGTCTGGATCCGGTGTTGTTCAAGG
ACCCGGTATCGGTGCAACGCAAGAAGTATCGCAGTCTAGAGAAGCCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR223710 protein sequence
Red=Cloning site Green=Tags(s)

MLWRRKSFWLALSAFWLLLVLLGVFPLRLAVSPGPLPGRSQGWPRWLDAAFLQSFSQSETNPEDVAQLPR
VSRGSSCTWGACFDTSKCRGKVLKIFVHSPAGPTSEAQRRILDSLEGSRYSALSPADACLLLFLPSQDRR
GACGPLPPNWNGGRNHLVLSLYPAPCTRLGQAMVAEASPSSDIFRPGFDIALPYLPEAHPLRGGAPGKLQ
QHSPQPGATLLAVAEEKGRWRITSTHASACLWDRHCEQDPGPQQTYPGETLPNATFCLIPGHRSATSCFL
QALQAGCIPVLLSPRWELPFSEVIDWTKAAIIADERLPLQVLAALREMLPSRVLALRQQTQFLWTAYFSS
VEKVIHTTLEVIQDRIWGASGHPSLMWNSPPGALLALPTFSTSLQDFPFYHLQLGSGPGSSFSAVIWVGA
SGESLLKLIQEVAGSRHCAQILILWNSEKPPPDRWPETAVPLTVIKGHRKVSNRFFPYSNISTNVILSLD
AQSTLSTSEVDFAFVVWQSFPERMVGFLSGSHFWDEAQGGWGYRTGMTNDFSMVLTTAAFYHRYYHTLFT
HSLPKALRTIADETPTCVDVLMNFLVATVTKLPPIKVPYGRQHPEAVPMDSGDPRPVPEPQPLDQDCINR
LAAGFGHMPLVSSQVRLDPVLFKDPVSVQRKKYRSLEKP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019578
ORF Size 2007 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019578.1
RefSeq Size 4129 bp
RefSeq ORF 2010 bp
Locus ID 56219
UniProt ID Q9JKV7
Cytogenetics 4 66.63 cM
MW 74 kDa
Summary Probable glycosyltransferase.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_019578" in other vectors (4)
SKU Description Size Price
MC220254 Extl1 (untagged) - Mouse exostoses (multiple)-like 1 (Extl1), (10ug) 10 ug
$675.00
MG223710 Extl1 (tGFP-tagged) - Mouse exostoses (multiple)-like 1 (Extl1), (10ug) 10 ug
$814.00
MR223710L3 Lenti ORF clone of Extl1 (Myc-DDK-tagged) - Mouse exostoses (multiple)-like 1 (Extl1) 10 ug
$914.00
MR223710L4 Lenti ORF clone of Extl1 (mGFP-tagged) - Mouse exostoses (multiple)-like 1 (Extl1) 10 ug
$914.00

Plasmid Map for MR223710

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR223710
Circular map for MR223710

file-alt Citations

Kruppel-Like Factor 4 represses osteoblast differentiation via ciliary Hedgehog signaling
Takeuchi, Kito, Itoh et al
Exp. Cell Res. (2018) 371 (2), 417-425 DOI: 10.1016/j.yexcr.2018.09.002
AI Summary: OriGene’s MYC and FLAG-tagged clones for mouse Mal, Mal2, Extl1, and Pxr were used as starting materials to generate retroviruses for gene expression studies in this paper.
Snippet: pMxs clones for generating retroviruses inducing mouse Mal, Mal2, Extl1, Pxr was prepared using the MYC and FLAG-tagged clones from Origene as a starting materials and pMxs vector as a destination vector (Thermo Fishers)
Showing 1-1 of 1 papers.
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