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Rasgrp4 (NM_001174155) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR223483
Rasgrp4 (Myc-DDK-tagged) - Mouse RAS guanyl releasing protein 4 (Rasgrp4), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $621.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rasgrp4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR223483 representing NM_001174155
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACCGGAAAGACATCAAAAGGAAGTCTCATCAGGAATGCTCTGGGAAGGCAGGAGGGCGGGGTCGGT
CACGTCAGGCCCGCCGCCACAAGACGTGCCCCACCCCCCGGGAAATCAGCAAGGTCATGGCGTCCATGAA
TCTGGGAGTGCTGAGTGAGAGCAGCTGCAGCGAAGATGAGCTATTGGAGGAATGTATCCGCTGCTTTGAC
TCGGCTGGCAGCCTGCGCCGAGGGGACCACATTCTCAAGATGGTGCTCACGATGCACAGCTGGGTGCTGC
CATCCTCAGAGCTCGCTGCCCGTCTGCTGACTTCGTATCAGAAGGCTGCCAAAGATGCCCAAGAGCTAAG
ACAGCTACAGATCTGTTACTTGGTCAGGTACTGGCTGACCCATCACCATGAGGCAGTGCACCAGGAACCC
CAGCTGGAAGCAGTCATAAGCCGGTTTTGGACCACTGTCGCTCAGGAGGGCAACATGGCCCAAAGGAGCC
TGGGAGATGCCTCCAGCCTGCTAAGTCCTGGAGGGCCCGGTCCCCCACCTCCCATGAGCAGCCCAGGCCT
GGGCAAGAAGCGGAAAGTGTCATTGCTATTCGATCACCTGGAGACAGAGGAGCTGGCACAACACCTCACT
TACCTGGAGTTCCGGTCTTTCCAGGCTATCACACCCCAAGACCTGCGGGGCTATGTTTTGCAGGGTTCAG
TGAGAGGTTGTCCAGCTCTGGAAGGTTCTGTGGGTCTGAGCAACAGTGTGTCCCGCTGGGTGCAGGTCAT
GGTGCTGAGTCGTCCTGGGCCTGCACAACGTGCCCAGGTCCTGGACAAATTCATTCGTGTGGCACAGAGG
CTTCACCAGCTGCAGAATTTCAACACACTGATGGCGGTCACGGGGGGTCTGTGTCATAGCGCCATCTCCA
GACTCAAGGACTCCCACGTTCATCTGAGCCCTGACAGCACAAAGGCCCTGCTGGAGCTGACAGAGCTCCT
CTCATCCCACAACAACTATGCCCACTACCGCCGCACCTGGGCCGGCTGTACTGGCTTCCGGCTGCCAGTA
CTGGGTGTGCACCTCAAGGACCTGGTATCTCTATATGAGGCTCATCCAGACAGATTGCCAGATGGCCGCC
TGCACCTACCCAAGCTGAATAGCCTCTATCTACGGCTGCAGGAGCTGATGGCGCTCCAGGGACAGCATCC
TCCCTGCAGTGCCAATGAGGACCTGCTGCACCTGCTGACGCTCTCCCTGGATCTCTTCTACACGGAAGAT
GAGATCTACGAGCTTTCTTATGCCCGGGAACCACGCTGTCCCAAGAGTCTGCCACCGTCCCCCTTCAAAG
CACCAGTGGTGGTAGAGTGGGCCCAGGGTGTGACACCAAAGCCAGACAGCGTGACTCTGGGTCAGCATGT
GGAACAGCTGGTGGAGTCCGTGTTCAAGAACTACGACCCAGAAGGCCGTGGCTCCATCTCTCTGGAGGAC
TTTGAGCGGCTGTCAGGCAACTTCCCGTTCGCCTGCCATGGGCTTCACCCACCTCCCCGCCATGGGAGTG
GCTCCTTCAGCAGAGAGGAGCTGACCAAGTACCTGCTCCATGCCAGTGCCATCTGCTCCAAGCTGGGCCT
GGCCTTCCTGCACGCCTTCCAGGAGGTCACCTTCCGAAAGCCCACGTTCTGTCACAGCTGCAGCGGCTTC
GTGAGCACTGGCCCTCTCTGGGGGGTCACCAAGCAAGGCTATCGCTGTCGGGACTGTGGGCTGTGTTGTC
ACAGACACTGCAGGGATCAAGTGAGAGTGGAGTGTAAGAAGAGGCCAGAGACTAAGGGTGACCCGGGCCC
CCCAGGTGCACCTGTGCCAGCCACATCACTTCCTCCTGCCAACTGTGGCTCAGAGGAAAGTCTCTCCTAT
ACACTCTCCCCGGATCCGGAGTCTGGTTGCCACCTTCGCCATGCTTGGACCCAGACGGAATCCTCACACT
CTTCCTGGGAGCCAGAGGTGGTGCCCTGCCCAGCACGGGTCTTACCATCCAGAGCTTCCTCGAAGCCCAG
CGTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR223483 representing NM_001174155
Red=Cloning site Green=Tags(s)

MNRKDIKRKSHQECSGKAGGRGRSRQARRHKTCPTPREISKVMASMNLGVLSESSCSEDELLEECIRCFD
SAGSLRRGDHILKMVLTMHSWVLPSSELAARLLTSYQKAAKDAQELRQLQICYLVRYWLTHHHEAVHQEP
QLEAVISRFWTTVAQEGNMAQRSLGDASSLLSPGGPGPPPPMSSPGLGKKRKVSLLFDHLETEELAQHLT
YLEFRSFQAITPQDLRGYVLQGSVRGCPALEGSVGLSNSVSRWVQVMVLSRPGPAQRAQVLDKFIRVAQR
LHQLQNFNTLMAVTGGLCHSAISRLKDSHVHLSPDSTKALLELTELLSSHNNYAHYRRTWAGCTGFRLPV
LGVHLKDLVSLYEAHPDRLPDGRLHLPKLNSLYLRLQELMALQGQHPPCSANEDLLHLLTLSLDLFYTED
EIYELSYAREPRCPKSLPPSPFKAPVVVEWAQGVTPKPDSVTLGQHVEQLVESVFKNYDPEGRGSISLED
FERLSGNFPFACHGLHPPPRHGSGSFSREELTKYLLHASAICSKLGLAFLHAFQEVTFRKPTFCHSCSGF
VSTGPLWGVTKQGYRCRDCGLCCHRHCRDQVRVECKKRPETKGDPGPPGAPVPATSLPPANCGSEESLSY
TLSPDPESGCHLRHAWTQTESSHSSWEPEVVPCPARVLPSRASSKPSV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001174155
ORF Size 2034 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001174155.1, NP_001167626.1
RefSeq Size 4636 bp
RefSeq ORF 2037 bp
Locus ID 233046
UniProt ID Q8BTM9
Cytogenetics 7 B1
MW 75.3 kDa
Summary Functions as a cation- and diacylglycerol (DAG)-regulated nucleotide exchange factor activating Ras through the exchange of bound GDP for GTP. May function in mast cells differentiation.UniProtKB/Swiss-Prot Function
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Documents
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"NM_001174155" in other vectors (4)
SKU Description Size Price
MC220374 Rasgrp4 (untagged) - Mouse RAS guanyl releasing protein 4 (Rasgrp4), transcript variant 1, (10ug) 10 ug
$684.00
MG223483 Rasgrp4 (tGFP-tagged) - Mouse RAS guanyl releasing protein 4 (Rasgrp4) transcript variant 1, (10ug) 10 ug
$821.00
MR223483L3 Lenti ORF clone of Rasgrp4 (Myc-DDK-tagged) - Mouse RAS guanyl releasing protein 4 (Rasgrp4), transcript variant 1 10 ug
$921.00
MR223483L4 Lenti ORF clone of Rasgrp4 (mGFP-tagged) - Mouse RAS guanyl releasing protein 4 (Rasgrp4), transcript variant 1 10 ug
$921.00

Plasmid Map for MR223483

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR223483
Circular map for MR223483

file-alt Citations

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