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Trmo (NM_029086) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR223346
5830415F09Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 5830415F09 gene (5830415F09Rik)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Trmo or NM_029086"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Trmo
Synonyms 5830415F09Rik; AV014846; Nap1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR223346 representing NM_029086
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGCGGCTTGGAGAAGCAGGGCTCGTGTGCTACGGCGGCCCCATGTGGGTGCGTTCAGCCGGCCCTGG
AGGCAGGGAATCTTTTAACTGAGCCAATAGGGTACTTGGAATCATGTTTCCCAGCCAAGATTGGCACTCC
AAGGCAGCCATCCATCTGTAGCCACTCCAGAGCCTGTTTGAAGATCAGAAAGAACATCTTTAATAACCCT
GAGCATTCCTTGATGGGCCTAGAAGAGTTTTCTCATGTTTGGATTTTGTTTGTTTTTCACAAAAATGGCC
ATTTGAACTACAAGGCAAAAGTGCAGCCGCCCAGGCTGAATGGGGCAAAGACTGGAGTGTTCTCCACACG
GAGCCCTCATCGCCCTAATGCCATAGGGCTGACGCTGGCCAAGCTGGAGAAGGTGGAAGGTGGAGCTGTC
TACCTGTCTGGAGTTGACATGATAGACGGCACACCTGTGCTAGACATAAAGCCCTACATTGCTGACTACG
ACTCACCACAGAACCTCAGTGTACACAATGACCACCATAAGCTGAGGGCTGAGGCCCAGGTTGATGGCAC
GGCCAACAGTTGTGACCAGCTGCTGCTCTCAGGGCGTGGGAAAGTACAGCCTCGCCAGAGCACCAAGGAG
AGACCGAAATGCCTTGAAGACAGGACCTCAGGAGAAAACTCTCAGAAGTCCAGAGACATGTCAGAAATCC
AGCACACTTTGCCAGAAGACAGAGAGAGAGCGTTGGATCTGGCACTGGAACCAAGCCGAGGTGAGAGCAT
GGATATGCCCGAAAATCAGCTTGGCCCACCGGAACTGAAGAGCTTTCTGGAAGAAGGCACAGATAGACCA
AGGAAAGTAGAAGGTGCCTTGGTCCTGCCGGGGAGCAGTGCAGAAACACAGTGGGATGCTTCCTACCGTG
CCAGGACAGCTGACAGAGTGCCCTACAGTGTTGTCCCCAGCTGGGTGACAGAGGCTCCTGTAGCCCCTTT
ACAAGTCCGGTTTACTCCTCACGCAGAGATGGATCTGAGGAAGCTCAATTCAGGAGATGCTAGTCAGCCG
TCATTTAAGTACTTCCACTCCGCAGAGGAAGCCAAGCGTGCCATTGAGGCTGTGCTGTCAGCAGATCCTC
GGTCTGTGTACCGCCGGAAGCTCTGCGAGGACCGCCTTTTCTTCTTCACTGTGGACACAGCACATGTCAC
CTGCTGGTTTGGTGATGGCTTCGCAGAGGTTGTGAGGATTAAACTGGCTTCTGAGTCTGTTCAGACTGTA
CTCCCTCCCTCTCCCTCTCCCAACGGAAGAGAAGAACAATCATCAGTGGAAGAGGAGGTGCAGCAGAGAG
CCGGACCCAGCAGGAGAGCAGCACCTAGAGCCGCTTCAATGCTCCTGACGCCCCAGTCCTCTAGCCTGGC
CACAAGTGCCCTTACCCACTGGGCCATCTCACTGCCCCCTCTCCCCCAAGGCCTGGTCTTTTCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR223346 representing NM_029086
Red=Cloning site Green=Tags(s)

MRGLEKQGSCATAAPCGCVQPALEAGNLLTEPIGYLESCFPAKIGTPRQPSICSHSRACLKIRKNIFNNP
EHSLMGLEEFSHVWILFVFHKNGHLNYKAKVQPPRLNGAKTGVFSTRSPHRPNAIGLTLAKLEKVEGGAV
YLSGVDMIDGTPVLDIKPYIADYDSPQNLSVHNDHHKLRAEAQVDGTANSCDQLLLSGRGKVQPRQSTKE
RPKCLEDRTSGENSQKSRDMSEIQHTLPEDRERALDLALEPSRGESMDMPENQLGPPELKSFLEEGTDRP
RKVEGALVLPGSSAETQWDASYRARTADRVPYSVVPSWVTEAPVAPLQVRFTPHAEMDLRKLNSGDASQP
SFKYFHSAEEAKRAIEAVLSADPRSVYRRKLCEDRLFFFTVDTAHVTCWFGDGFAEVVRIKLASESVQTV
LPPSPSPNGREEQSSVEEEVQQRAGPSRRAAPRAASMLLTPQSSSLATSALTHWAISLPPLPQGLVFS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_029086
ORF Size 1464 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_029086.2, NP_083362.1
RefSeq Size 1558 bp
RefSeq ORF 1467 bp
Locus ID 74753
UniProt ID Q562D6
Cytogenetics 4 B1
MW 54.1 kDa
Summary S-adenosyl-L-methionine-dependent methyltransferase responsible for the addition of the methyl group in the formation of N6-methyl-N6-threonylcarbamoyladenosine at position 37 (m(6)t(6)A37) of the tRNA anticodon loop of tRNA(Ser)(GCU). The methyl group of m(6)t(6)A37 may improve the efficiency of the tRNA decoding ability. May bind to tRNA.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_029086" in other vectors (4)
SKU Description Size Price
MC216862 5830415F09Rik (untagged) - Mouse RIKEN cDNA 5830415F09 gene (5830415F09Rik), (10ug) 10 ug
$503.00
MG223346 5830415F09Rik (tGFP-tagged) - Mouse RIKEN cDNA 5830415F09 gene (5830415F09Rik), (10ug) 10 ug
$657.00
MR223346L3 Lenti ORF clone of 5830415F09Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 5830415F09 gene (5830415F09Rik) 10 ug
$757.00
MR223346L4 Lenti ORF clone of 5830415F09Rik (mGFP-tagged) - Mouse RIKEN cDNA 5830415F09 gene (5830415F09Rik) 10 ug
$757.00

Plasmid Map for MR223346

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR223346
Circular map for MR223346

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